Name: Anti-LSP1 antibody [EPR5997]
SKU: ab133506
Rating: 5 (1 reviews)

Rabbit Recombinant Monoclonal LSP1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (AB133506), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Not recommended	Tested	Tested	Tested
Mouse	Tested	Not recommended	Tested	Expected	Expected
Rat	Tested	Not recommended	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/250 - 1/500	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Rat	Dilution info 1/250 - 1/500	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Human	Dilution info 1/250 - 1/500	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/10000 - 1/50000	Notes -
Species Rat	Dilution info 1/10000 - 1/50000	Notes -
Species Human	Dilution info 1/10000 - 1/50000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes For unpurified use at 1/500 - 1/1000.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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1 product for Alternative Product

Target data

See full target information LSP1

Function

May play a role in mediating neutrophil activation and chemotaxis.

Alternative names

WP34, LSP1, Lymphocyte-specific protein 1, 47 kDa actin-binding protein, 52 kDa phosphoprotein, Lymphocyte-specific antigen WP34, pp52

Recommended products

Rabbit Recombinant Monoclonal LSP1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR5997
Purification technique: Affinity purification Protein A
Dissociation constant: 1.02 x 10^-10 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Storage information: Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Lymphocyte-specific protein 1 also known as LSP1 is a 52 kDa intracellular F-actin binding protein. It plays a role in cytoskeletal organization and cell motility. This protein has significant expression in lymphocytes neutrophils macrophages and endothelial cells. LSP1 modulates several cellular functions including adhesion and migration by interacting with the actin cytoskeleton. Known alternatively as pp52 and WD-40 repeat-containing protein 1 LSP1 acts as a link connecting signal transduction from cell surface receptors to cytoskeletal regions.

Biological function summary

LSP1 influences immune cell regulation and inflammatory response. By acting as an important player in the modulation of actin polymerization it contributes to leukocyte chemotaxis and transmigration. LSP1 forms part of a larger protein complex involved in reorganizing the cytoskeleton facilitating cellular dynamics necessary for immune response. Additionally interactions with adapter proteins like 14-3-3 add another level of functional regulation.

Pathways

LSP1 integrates into the chemokine signaling pathway and the P38 MAPK signaling pathway. In these pathways it exerts influence on cellular processes such as migration and response to external stimuli. It shows connections with proteins such as CXCR4 and MAPK highlighting its role in conveying chemotactic signals that direct immune cells toward inflammatory sites. These interactions are integral in establishing directionality and speed of cell movement in response to chemokine gradients.

Associated diseases and disorders

Aberrations in LSP1 function or expression link to autoimmune conditions like systemic lupus erythematosus and vascular disorders like atherosclerosis. Its role in mediating vascular permeability and leukocyte migration suggests that dysregulation can contribute to pathogenic inflammation. Related proteins like MCP-1 and ICAM-1 participate in these disease pathways highlighting the involvement of LSP1 in modulating endothelial responses and immune cell recruitment at sites of inflammation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-LSP1 antibody [EPR5997] (ab133506)
All lanes: Western blot - Anti-LSP1 antibody [EPR5997] (ab133506) at 1/10000 dilution
All lanes: Rat brain lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Western blot - Anti-LSP1 antibody [EPR5997] (ab133506)
All lanes: Western blot - Anti-LSP1 antibody [EPR5997] (ab133506) at 1/10000 dilution
Lane 1: Mouse spleen lysates at 20 µg
Lane 2: Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg
Lane 3: HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling LSP1 with purified ab133506 at 1:500 dilution (1.954 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-LSP1 antibody [EPR5997] (ab133506)
Immunocytochemistry/ Immunofluorescence analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling LSP1 with purified ab133506 at 1:100 dilution (9.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-LSP1 antibody [EPR5997] (ab133506)
All lanes: Western blot - Anti-LSP1 antibody [EPR5997] (ab133506) at 1/10000 dilution
Lane 1: Daudi cell lysate at 10 µg
Lane 2: Ramos cell lysate at 10 µg
Lane 3: HuT78 cell lysate at 10 µg
Secondary
All lanes: Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 37 kDa
Observed band size: 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling LSP1 with ab133506 at 1/250 dilution.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
OI-RD Scanning - Anti-LSP1 antibody [EPR5997] (ab133506)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSP1 antibody [EPR5997] (ab133506)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling LSP1 with ab133506 at 1/250 dilution.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-LSP1 antibody [EPR5997] (ab133506)
Intracellular Flow Cytometry analysis of Daudi (Human Burkitt's lymphoma lymphoblast) cells labeling LSP1 with purified ab133506 at 1/100 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).