AB191180

Anti-LSR antibody

Name: Anti-LSR antibody (ab191180) | Abcam
Brand: Abcam Limited
SKU: AB191180
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal LSR antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human LSR aa 450-500.

View Alternative Names

ILDR3, LISCH, LSR, Lipolysis-stimulated lipoprotein receptor, Angulin-1

2 Images

Supplier Data

Immunoprecipitation - Anti-LSR antibody (AB191180)

Immunoprecipitation of 293T whole cell lysate using ab191180 (lane 1) at 6μg per reaction (0.5 or 1 mg/IP; 20% of IP loaded/lane). Immunoblot analysis of LSR utilised ab191180 at 0.4 μg/ml. The lysate in lane 2 is precipitated with an IgG control antibody. Detection utilised chemiluminescence with an exposure time of 30seconds.

All lanes:

Immunoprecipitation - Anti-LSR antibody (ab191180)

Predicted band size: 71 kDa

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Supplier Data

Western blot - Anti-LSR antibody (AB191180)

All lanes:

Western blot - Anti-LSR antibody (ab191180) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate prepared using RIPA lysis buffer. at 50 µg

Lane 2:

293T whole cell lysate prepared using RIPA lysis buffer. at 50 µg

Lane 3:

Jurkat whole cell lysate prepared using RIPA lysis buffer. at 50 µg

Predicted band size: 71 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human LSR aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

Q86X29

Specificity

ab191180 was affinity purified using an epitope specific to LSR immobilized on a solid support.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LSR protein also known as Lipolysis Stimulated Lipoprotein Receptor functions mechanically as a component of the plasma membrane where it assists in lipid metabolism. It has a mass of approximately 68 kDa and plays an important role in endocytosis and binding of apolipoproteins. LSR is expressed significantly in liver and intestinal tissues. Researchers often study it for its involvement in lipid management in the human body.

Biological function summary

LSR interacts with other proteins to form a multimeric complex that regulates lipid processing. Its presence is essential for maintaining lipid homeostasis by binding chylomicrons and cholesterol-rich lipoproteins. The LSR complex plays a significant role in managing body lipid levels and maintaining cellular health. These processes contribute to the efficient transport and clearance of dietary fats which are essential functions in metabolic regulation.

Pathways

The activity of the LSR protein is integral to the lipid metabolism and cholesterol homeostasis pathways. Within these pathways LSR collaborates with proteins like APOB and APOE to facilitate lipid uptake and processing. It functions by enabling the clearance of chylomicron remnants and very low-density lipoprotein particles from the bloodstream maintaining lipid balance and preventing disorders related to lipid accumulation.

The dysfunction or altered expression of LSR can associate with hyperlipidemia and atherosclerosis. These conditions result from impaired lipid uptake and clearance leading to elevated blood lipid levels. LSR's interaction with apolipoproteins such as APOB links its activities to the metabolic disturbances observed in these diseases. Understanding LSR's role in lipid mechanisms provides potential avenues for therapeutic interventions targeting lipid-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Probable role in the clearance of triglyceride-rich lipoprotein from blood. Binds chylomicrons, LDL and VLDL in presence of free fatty acids and allows their subsequent uptake in the cells (By similarity). Maintains epithelial barrier function by recruiting MARVELD2/tricellulin to tricellular tight junctions (By similarity).

See full target information LSR

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Haematologica 108:2513-2519 PubMed36727398

2023

Inhibition of menin, BCL-2, and FLT3 combined with a hypomethylating agent cures -ITD/-TKD mutant acute myeloid leukemia in a patient-derived xenograft model.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Z Carter,Po Yee Mak,Wenjing Tao,Lauren B Ostermann,Duncan H Mak,Baozhen Ke,Peter Ordentlich,Gerard M McGeehan,Michael Andreeff

Journal of cellular biochemistry 121:4642-4653 PubMed32048750

2020

Potentiation of cancerous progression by LISCH7 via direct stimulation of TGFB1 transcription in triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojiang Tang,Yuhui Zhou,Yang Liu,Wei Zhang,Chao Liu,Changyou Yan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LSR antibody

Immunoprecipitation - Anti-LSR antibody (AB191180)

Western blot - Anti-LSR antibody (AB191180)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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