AB140124

Anti-LSS antibody [EPR6703]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal LSS antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

OSC, LSS, Lanosterol synthase, Oxidosqualene--lanosterol cyclase, hOSC

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSS antibody [EPR6703] (AB140124)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSS antibody [EPR6703] (AB140124)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling LSS with ab140124 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSS antibody [EPR6703] (AB140124)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling LSS with ab140124 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-LSS antibody [EPR6703] (AB140124)

All lanes:

Western blot - Anti-LSS antibody [EPR6703] (ab140124) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 83 kDa

false

Carrier free

Anti-LSS antibody [EPR6703] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6703

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LSS protein also known as lanosterol synthase plays a mechanical role in the biosynthesis of sterols. Its main function involves the cyclization of 23-oxidosqualene to lanosterol. LSS has a molecular weight of approximately 83 kDa. It is expressed widely across various tissues with notable presence in the liver adrenal glands and reproductive organs. This enzyme resides within the endoplasmic reticulum membrane reflecting its involvement in cholesterol synthesis.

Biological function summary

LSS catalyzes an important step in the conversion of squalene a precursor to the steroid skeleton into lanosterol. This function places LSS as an important contributor in the cholesterol biosynthesis pathway. LSS forms part of the sterol biosynthesis complex interacting with other enzymes to facilitate the production of essential sterols. By producing lanosterol LSS acts as a precursor gateway for other steroid hormones and molecules.

Pathways

Cholesterol homeostasis and steroidogenesis heavily depend on LSS activity. The enzyme operates within the mevalonate pathway an important cholesterol-producing route and is essential for the synthesis of isoprenoids. It interacts closely with HMG-CoA reductase an important regulator of cholesterol levels highlighting its central role in maintaining cellular lipid balance. Through its enzymatic action LSS links the synthesis of cholesterol with downstream steroid hormones.

LSS has associations with conditions such as lathosterolosis a rare autosomal recessive disorder affecting cholesterol biosynthesis. Mutations in the LSS gene disrupt normal cholesterol production contributing to developmental abnormalities. Additionally LSS has connections to hypercholesterolemia having indirect relations through the cholesterol metabolism pathway which also involves the LDL receptor. These dysfunctions highlight the importance of LSS in maintaining systemic lipid homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key enzyme in the cholesterol biosynthesis pathway. Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus (PubMed : 14766201, PubMed : 26200341, PubMed : 7639730). Through the production of lanosterol may regulate lens protein aggregation and increase transparency (PubMed : 26200341).

See full target information LSS

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:704 PubMed39349433

2024

Cholesterol inhibition enhances antitumor response of gilteritinib in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chao-Yue Sun,Di Cao,Yue-Ning Wang,Nuo-Qing Weng,Qian-Nan Ren,Shuo-Cheng Wang,Mei-Yin Zhang,Shi-Juan Mai,Hui-Yun Wang

American journal of human genetics 103:777-785 PubMed30401459

2018

Bi-allelic Mutations in LSS, Encoding Lanosterol Synthase, Cause Autosomal-Recessive Hypotrichosis Simplex.

Applications

Unspecified application

Species

Unspecified reactive species

Maria-Teresa Romano,Aylar Tafazzoli,Maximilian Mattern,Sugirthan Sivalingam,Sabrina Wolf,Alexander Rupp,Holger Thiele,Janine Altmüller,Peter Nürnberg,Jürgen Ellwanger,Reto Gambon,Alessandra Baumer,Nicolai Kohlschmidt,Dieter Metze,Stefan Holdenrieder,Ralf Paus,Dieter Lütjohann,Jorge Frank,Matthias Geyer,Marta Bertolini,Pavlos Kokordelis,Regina C Betz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LSS antibody [EPR6703]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSS antibody [EPR6703] (AB140124)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSS antibody [EPR6703] (AB140124)

Western blot - Anti-LSS antibody [EPR6703] (AB140124)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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