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Rabbit Recombinant Monoclonal LSS antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-LSS antibody [EPR6704(B)] - BSA and Azide free (AB248003), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSS antibody [EPR6704(B)] - BSA and Azide free (AB248003), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-LSS antibody [EPR6704(B)] - BSA and Azide free (AB248003), expandable thumbnail
  • OI-RD Scanning - Anti-LSS antibody [EPR6704(B)] - BSA and Azide free (AB248003), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Key enzyme in the cholesterol biosynthesis pathway. Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus (PubMed:14766201, PubMed:26200341, PubMed:7639730). Through the production of lanosterol may regulate lens protein aggregation and increase transparency (PubMed:26200341).

Alternative names

OSC, LSS, Lanosterol synthase, Oxidosqualene--lanosterol cyclase, hOSC

Recommended products

Rabbit Recombinant Monoclonal LSS antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR6704(B)
Purification technique
Affinity purification Protein A
Dissociation constant
6.2 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248003 is the carrier-free version of Anti-LSS antibody [EPR6704(B)] ab124785.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The LSS protein also known as lanosterol synthase plays a mechanical role in the biosynthesis of sterols. Its main function involves the cyclization of 23-oxidosqualene to lanosterol. LSS has a molecular weight of approximately 83 kDa. It is expressed widely across various tissues with notable presence in the liver adrenal glands and reproductive organs. This enzyme resides within the endoplasmic reticulum membrane reflecting its involvement in cholesterol synthesis.

Biological function summary

LSS catalyzes an important step in the conversion of squalene a precursor to the steroid skeleton into lanosterol. This function places LSS as an important contributor in the cholesterol biosynthesis pathway. LSS forms part of the sterol biosynthesis complex interacting with other enzymes to facilitate the production of essential sterols. By producing lanosterol LSS acts as a precursor gateway for other steroid hormones and molecules.

Pathways

Cholesterol homeostasis and steroidogenesis heavily depend on LSS activity. The enzyme operates within the mevalonate pathway an important cholesterol-producing route and is essential for the synthesis of isoprenoids. It interacts closely with HMG-CoA reductase an important regulator of cholesterol levels highlighting its central role in maintaining cellular lipid balance. Through its enzymatic action LSS links the synthesis of cholesterol with downstream steroid hormones.

Associated diseases and disorders

LSS has associations with conditions such as lathosterolosis a rare autosomal recessive disorder affecting cholesterol biosynthesis. Mutations in the LSS gene disrupt normal cholesterol production contributing to developmental abnormalities. Additionally LSS has connections to hypercholesterolemia having indirect relations through the cholesterol metabolism pathway which also involves the LDL receptor. These dysfunctions highlight the importance of LSS in maintaining systemic lipid homeostasis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-LSS antibody [EPR6704(B)] - BSA and Azide free (ab248003), expandable thumbnail

    Western blot - Anti-LSS antibody [EPR6704(B)] - BSA and Azide free (ab248003)

    This data was developed using Anti-LSS antibody [EPR6704(B)] ab124785, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-LSS antibody [EPR6704(B)] (Anti-LSS antibody [EPR6704(B)] ab124785) at 1/50000 dilution

    Lane 1: SH-SY5Y cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: HT-1376 cell lysate at 10 µg

    Lane 4: Human fetal liver tissue lysate at 10 µg

    Secondary

    All lanes: Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 83 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSS antibody [EPR6704(B)] - BSA and Azide free (ab248003), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LSS antibody [EPR6704(B)] - BSA and Azide free (ab248003)

    This data was developed using Anti-LSS antibody [EPR6704(B)] ab124785, the same antibody clone in a different buffer formulation.

    Anti-LSS antibody [EPR6704(B)] ab124785 at 1/50 dilution staining LSS in paraffin-embedded Human kidney tissues by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-LSS antibody [EPR6704(B)] - BSA and Azide free (ab248003), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-LSS antibody [EPR6704(B)] - BSA and Azide free (ab248003)

    This data was developed using Anti-LSS antibody [EPR6704(B)] ab124785, the same antibody clone in a different buffer formulation.

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling LSS with purified Anti-LSS antibody [EPR6704(B)] ab124785 at 1/100 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • OI-RD Scanning - Anti-LSS antibody [EPR6704(B)] - BSA and Azide free (ab248003), expandable thumbnail

    OI-RD Scanning - Anti-LSS antibody [EPR6704(B)] - BSA and Azide free (ab248003)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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