Anti-LST1 antibody [7E2] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LST1 antibody [7E2] - BSA and Azide free (AB255833)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling LST1 with ab252839 at 4.32μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human tonsil tissue is observed. The section was incubated with ab252839 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252839).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LST1 antibody [7E2] - BSA and Azide free (AB255833)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling LST1 with ab252839 at 4.32μg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse spleen tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252839).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LST1 antibody [7E2] - BSA and Azide free (AB255833)

Immunohistochemical analysis of paraffin-embedded rat thymus tissue labeling LST1 with ab252839 at 4.32μg/ml followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on rat thymus tissue is observed. The section was incubated with ab252839 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252839).

• WB

Supplier Data

Western blot - Anti-LST1 antibody [7E2] - BSA and Azide free (AB255833)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 mins.

The expression molecular weight observed is consistent with what has been described in the literature (PMID : 19663701).

This blot was developed using a higher sensitivity ECL substrate.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252839).

All lanes:

Western blot - Anti-LST1 antibody [7E2] (<a href='/en-us/products/primary-antibodies/lst1-antibody-7e2-ab252839'>ab252839</a>) at 0.432 µg/mL

All lanes:

HEK-293 (human embryonic kidney epithelial cell) lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/100000 dilution

Predicted band size: 11 kDa

true

Exposure time: 3min