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AB288417

Anti-LTA antibody [3F12.2D3]

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Mouse Recombinant Monoclonal TNF beta antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LTA.

View Alternative Names

TNFB, TNFSF1, LTA, Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1

1 Images
Western blot - Anti-LTA antibody [3F12.2D3] (AB288417)
  • WB

Supplier Data

Western blot - Anti-LTA antibody [3F12.2D3] (AB288417)

The image data was generated using a rabbit IgG-chimeric version of the same antibody clone

The expected unmodified running size for Lymphotoxin alpha is 22.3kDa, though the form shown here is likely glycosylated.

All lanes:

Western blot - Anti-LTA antibody [3F12.2D3] (ab288417) at 2 µg/mL

All lanes:

LPS-treated human peripheral blood monocyte (PBM) lysate (in RIPA buffer) at 35 µg

Predicted band size: 22 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3F12.2D3

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human LTA.

P01374

Specificity

3F12 binds to human LTα3 and LTαβ (with a KD of ~0.3 nM, determined by BIACORE; ~ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Proclin 300 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM (PubMed : 9462508). In its heterotrimeric form with LTB binds to TNFRSF3/LTBR (PubMed : 24248355). Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.
See full target information LTA
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