Mouse Recombinant Monoclonal TNF beta antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LTA.
Images
![Western blot - Anti-LTA antibody [3F12.2D3] (AB288417), expandable thumbnail](https://content.abcam.com/products/images/lta-antibody-3f122d3-ab288417--western-blot-img176811.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LTA antibody [3F12.2D3] (AB288417)")
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Proclin 300
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human LTA. Database link P01374
Abcam Recommends
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mL | Notes - |
Target data
Function
Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM (PubMed:9462508). In its heterotrimeric form with LTB binds to TNFRSF3/LTBR (PubMed:24248355). Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.
Alternative names
TNFB, TNFSF1, LTA, Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1
Recommended products
Mouse Recombinant Monoclonal TNF beta antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LTA.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Proclin 300
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human LTA. Database link P01374
- Clone number
- 3F12.2D3
- Purification technique
- Affinity purification Protein A
- Specificity
3F12 binds to human LTα3 and LTαβ (with a KD of ~0.3 nM, determined by BIACORE; ~ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms.
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-LTA antibody [3F12.2D3] (ab288417), expandable thumbnail](https://content.abcam.com/products/images/lta-antibody-3f122d3-ab288417--western-blot-img176811.jpg "Western blot - Anti-LTA antibody [3F12.2D3] (ab288417)")
Western blot - Anti-LTA antibody [3F12.2D3] (ab288417)
The image data was generated using a rabbit IgG-chimeric version of the same antibody clone
The expected unmodified running size for Lymphotoxin alpha is 22.3kDa, though the form shown here is likely glycosylated.
All lanes: Western blot - Anti-LTA antibody [3F12.2D3] (ab288417) at 2 µg/mL
All lanes: LPS-treated human peripheral blood monocyte (PBM) lysate (in RIPA buffer) at 35 µg
Predicted band size: 22 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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