Rabbit Recombinant Monoclonal TNF beta antibody. Carrier free. Suitable for sELISA and reacts with Human, Rhesus monkey samples.
Images
![Sandwich ELISA - Anti-LTA antibody [EPR20507-260] - BSA and Azide free (Detector) (AB244964), expandable thumbnail](https://content.abcam.com/products/images/lta-antibody-epr20507-260-bsa-and-azide-free-detector-ab244964--sandwich-elisa-img51494.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-LTA antibody [EPR20507-260] - BSA and Azide free (Detector) (AB244964)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
| Rhesus monkey | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rhesus monkey | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM (PubMed:9462508). In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo.
Alternative names
Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, LTA, TNFSF1, TNFB
Recommended products
Rabbit Recombinant Monoclonal TNF beta antibody. Carrier free. Suitable for sELISA and reacts with Human, Rhesus monkey samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR20507-260
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab244964 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human TNF beta. The recommended pair for sandwich ELISA is:
Capture: Anti-LTA antibody [EPR20507-215] - BSA and Azide free (Capture) ab244840, Human TNF beta Capture Antibody (unconjugated)
Detector: ab244964, Human TNF beta Detector Antibody (unconjugated)
The reference range value is 16 - 1000 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-LTA antibody [EPR20507-260] - BSA and Azide free (Detector) (ab244964), expandable thumbnail](https://content.abcam.com/products/images/lta-antibody-epr20507-260-bsa-and-azide-free-detector-ab244964--sandwich-elisa-img51494.jpg "Sandwich ELISA - Anti-LTA antibody [EPR20507-260] - BSA and Azide free (Detector) (ab244964)")
Sandwich ELISA - Anti-LTA antibody [EPR20507-260] - BSA and Azide free (Detector) (ab244964)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Human TNF beta ELISA Kit ab229202).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com