Anti-LTB antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LTB antibody (AB64835)

ab64835 at 1/500 - 1/1000 dilution staining Lymphotoxin Beta in HeLa cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.

• WB

Unknown

Western blot - Anti-LTB antibody (AB64835)

The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.

All lanes:

Western blot - Anti-LTB antibody (ab64835) at 1/500 dilution

Lane 1:

HepG2 cell extract

Lane 2:

HepG2 cell extract with immunising peptide

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

• WB

CiteAb

Western blot - Anti-LTB antibody (AB64835)

LTB western blot using anti-LTB antibody ab64835. Publication image and figure legend from Simonin, Y., Vegna, S., et al., 2013, PLoS Pathog, PubMed 23555249.

ab64835 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab64835 please see the product overview.

Upregulation of lymphotoxin signaling in HCV cellular models.Protein and RNA extracts from exponentially growing full-length (Nneo/C-5B) replicon lines, the infectious HCV model and the corresponding control cell lines, Huh7 and Huh7.5.1, respectively, were analyzed by RT-qPCR and by immunoblot. (A) Analysis of LTα, LTβ, LTβR and CXCL10 mRNA expression and representative protein expression of LTβ in the Nneo/C-5B replicon propagated in Huh7 cells and in JFH1-infected Huh7.5.1 cells (B) Representative immunoblots of 3 independent experiments are shown. Where appropriate, results are presented as mean+/- SEM of 3 separate experiments (*p<0.01, **p<0.001).

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