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AB78294

Anti-LTBP1 antibody

5

(1 Review)

|

(12 Publications)

Rabbit Polyclonal LTBP1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 12 publications.

View Alternative Names

Latent-transforming growth factor beta-binding protein 1, LTBP-1, Transforming growth factor beta-1-binding protein 1, TGF-beta1-BP-1, LTBP1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LTBP1 antibody (AB78294)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LTBP1 antibody (AB78294)

IHC image of LTBP1 staining in Mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78294, 5μg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Western blot - Anti-LTBP1 antibody (AB78294)
  • WB

Unknown

Western blot - Anti-LTBP1 antibody (AB78294)

Based on the immunogen sequence, this antibody is predicted to react with all three isoforms of LTBP1. The band detected here might correspond to the long isoform, which is 187 kDa and is found in fibroblasts.

All lanes:

Western blot - Anti-LTBP1 antibody (ab78294) at 1 µg/mL

Lane 1:

Liver (Rat) Tissue Lysate at 10 µg

Lane 2:

Pancreas (Mouse) Tissue Lysate at 10 µg

Lane 3:

Spleen (Mouse) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 187 kDa

Observed band size: 187 kDa

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Western blot - Anti-LTBP1 antibody (AB78294)
  • WB

Lab

Western blot - Anti-LTBP1 antibody (AB78294)

All lanes:

Western blot - Anti-LTBP1 antibody (ab78294) at 1 µg/mL

Lane 1:

Liver (Rat) Tissue Lysate at 20 µg

Lane 2:

Spleen (Mouse) Tissue Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 187 kDa

Observed band size: 171 kDa,187 kDa,55 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LTBP1 or Latent Transforming Growth Factor Beta Binding Protein 1 mechanically acts as an important regulator in the activation and release of Transforming Growth Factor Beta (TGF-β). It is part of the latent TGF-β complex binding to TGF-β to help control its availability in the extracellular matrix. LTBP1 has a molecular weight of approximately 190 kDa. The protein is expressed in many tissues including the lung skin and heart highlighting its widespread role in various physiological processes.
Biological function summary

Its main role is modulating the bioavailability and activity of TGF-β a protein important to cellular differentiation proliferation and apoptosis. As part of a larger latent TGF-β complex it determines the precise activation and function of TGF-β by anchoring it to the extracellular matrix. This regulation is important for maintaining tissue homeostasis and has implications in developmental and repair processes across different organ systems.

Pathways

LTBP1 plays a significant role in the TGF-β signaling pathway which is important for controlling cellular growth and differentiation. It is involved in linking TGF-β to large latent complexes impacting its sequestration and activation within the matrix. Through this pathway LTBP1 interacts with proteins such as integrins which help release the active form of TGF-β furthering downstream signaling activities.

LTBP1 is implicated in fibrotic diseases and certain cancers. Abnormal expression or function of LTBP1 can lead to dysregulation of TGF-β activity causing excessive tissue fibrosis as seen in systemic sclerosis or contributing to the progression of cancers like lung carcinoma. The interaction of LTBP1 with other proteins particularly integrins can exacerbate these conditions by derailing typical matrix remodeling and cellular signaling processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space (PubMed : 2022183, PubMed : 8617200, PubMed : 8939931). Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta (PubMed : 15184403, PubMed : 8617200, PubMed : 8939931). Outcompeted by LRRC32/GARP for binding to LAP regulatory chain of TGF-beta (PubMed : 22278742).
See full target information LTBP1
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 80:288 PubMed37689587

2023

TGFβ signaling links early life endocrine-disrupting chemicals exposure to suppression of nucleotide excision repair in rat myometrial stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Victoria Bariani,Yan-Hong Cui,Mohamed Ali,Tao Bai,Sandra L Grimm,Cristian Coarfa,Cheryl L Walker,Yu-Ying He,Qiwei Yang,Ayman Al-Hendy

BMC pulmonary medicine 23:300 PubMed37582718

2023

Bioinformatics analysis of the immune cell infiltration characteristics and correlation with crucial diagnostic markers in pulmonary arterial hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Guili Lian,Jingxian You,Weijun Lin,Gufeng Gao,Changsheng Xu,Huajun Wang,Li Luo

The European respiratory journal 61: PubMed36758986

2023

Regulating the cell shift of endothelial cell-like myofibroblasts in pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuju Wu,Daoqin Zhang,Xiaojing Qiao,Li Zhang,Xinjiang Cai,Jaden Ji,Jocelyn A Ma,Yan Zhao,John A Belperio,Kristina I Boström,Yucheng Yao

Scientific reports 13:6865 PubMed37100863

2023

Intravitreal injection of fibrillin 2 (Fbn2) recombinant protein for therapy of retinopathy in a retina-specific Fbn2 knock-down mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Xue Zhang,Ying Wen,Da Dong Guo,Fu Ru Xu,Gui Min Wang,Xing Rong Wang,Yong Wei Shi,Jie Ding,Qian Jiang,Wen Jun Jiang,Jost B Jonas,Hong Sheng Bi

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 55:e12206 PubMed36629522

2023

Immune suppressive signaling regulated by latent transforming growth factor beta binding protein 1 promotes metastasis in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Gu,Wei Wang,Changdong Sun,Li Ding,Li Li,Peng Shu,Jun Xu

The Journal of clinical investigation 131: PubMed34779414

2021

Candesartan prevents arteriopathy progression in cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy model.

Applications

Unspecified application

Species

Unspecified reactive species

Taisuke Kato,Ri-Ichiroh Manabe,Hironaka Igarashi,Fuyuki Kametani,Sachiko Hirokawa,Yumi Sekine,Natsumi Fujita,Satoshi Saito,Yusuke Kawashima,Yuya Hatano,Shoichiro Ando,Hiroaki Nozaki,Akihiro Sugai,Masahiro Uemura,Masaki Fukunaga,Toshiya Sato,Akihide Koyama,Rie Saito,Atsushi Sugie,Yasuko Toyoshima,Hirotoshi Kawata,Shigeo Murayama,Masaki Matsumoto,Akiyoshi Kakita,Masato Hasegawa,Masafumi Ihara,Masato Kanazawa,Masatoyo Nishizawa,Shoji Tsuji,Osamu Onodera

JCI insight 5: PubMed31343988

2019

Fibulin-1c regulates transforming growth factor-β activation in pulmonary tissue fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Liu,Marion A Cooley,Andrew G Jarnicki,Theo Borghuis,Prema M Nair,Gavin Tjin,Alan C Hsu,Tatt Jhong Haw,Michael Fricker,Celeste L Harrison,Bernadette Jones,Nicole G Hansbro,Peter A Wark,Jay C Horvat,W Scott Argraves,Brian G Oliver,Darryl A Knight,Janette K Burgess,Philip M Hansbro

PloS one 11:e0167474 PubMed27907131

2016

iTRAQ-Based Quantitative Proteomic Comparison of Early- and Late-Passage Human Dermal Papilla Cell Secretome in Relation to Inducing Hair Follicle Regeneration.

Applications

IHC-P

Species

Unspecified reactive species

Huan Zhang,Ning-Xia Zhu,Keng Huang,Bo-Zhi Cai,Yang Zeng,Yan-Ming Xu,Yang Liu,Yan-Ping Yuan,Chang-Min Lin

FASEB journal : official publication of the Federa 29:4956-67 PubMed26405299

2015

Extracellular matrix-induced Hic-5 expression in glomerular mesangial cells leads to a prosclerotic phenotype independent of TGF-β.

Applications

Unspecified application

Species

Unspecified reactive species

Nick Hornigold,Andrew Mooney

Matrix biology : journal of the International Society for Matrix Biology 43:61-70 PubMed25805620

2015

Abrogation of both short and long forms of latent transforming growth factor-β binding protein-1 causes defective cardiovascular development and is perinatally lethal.

Applications

Unspecified application

Species

Unspecified reactive species

Masahito Horiguchi,Vesna Todorovic,Krassimira Hadjiolova,Ralf Weiskirchen,Daniel B Rifkin
View all publications
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