Rabbit Polyclonal LTBP4 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LTBP4 aa 950-1200.
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- Cell reports 33:1084312020Noise Exposures Causing Hearing Loss Generate Proteotoxic Stress and Activate the Proteostasis Network.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNopporn Jongkamonwiwat,Miguel A Ramirez,Seby Edassery,Ann C Y Wong,Jintao Yu,Tirzah Abbott,Kwang Pak,Allen F Ryan,Jeffrey N SavasPubMed 33238128
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human LTBP4 aa 950-1200. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N2S1
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Target data
Function
Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space. Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta.
Alternative names
Latent-transforming growth factor beta-binding protein 4, LTBP-4, LTBP4
Recommended products
Rabbit Polyclonal LTBP4 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LTBP4 aa 950-1200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human LTBP4 aa 950-1200. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N2S1
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein LTBP4 also known as Latent Transforming Growth Factor Beta Binding Protein 4 plays an important mechanical role in the extracellular matrix. It is involved in the storage and activation of TGF-beta. Weighing approximately 210 kDa this protein is mainly expressed in connective tissues including skin lung and kidney tissues. It helps ensure proper assembly of elastic fibers and is important in maintaining the structural integrity of the extracellular matrix.
- Biological function summary
LTBP4 associates with proteins to form larger complexes and regulates the availability of TGF-beta within the cell environment. Its function relates closely to modulating growth factors that control cell proliferation differentiation and migration. LTBP4's role in elastic fiber structure links it to essential biological processes such as tissue repair and fibrosis. Its presence within the extracellular matrix implies strong interactions with other structural proteins that lead to the proper function of connective tissue.
- Pathways
LTBP4 directly influences the TGF-beta signaling pathway important for developmental processes and homeostasis in tissues. The protein plays a part in regulating this pathway affecting how TGF-beta interacts with its receptors and consequently impacts Smad-dependent and independent signaling cascades. It is associated with other proteins like fibrillin-1 which contributes to the formation and maintenance of the extracellular microfibril structure supporting TGF-beta activation.
- Associated diseases and disorders
LTBP4 shows a strong connection to connective tissue disorders such as cutis laxa where mutations in LTBP4 result in skin elasticity issues. Additionally its function ties to pulmonary emphysema a condition characterized by tissue degeneration in the lungs partly due to compromised extracellular matrix integrity. LTBP4's interactions with proteins like elastin become critical in understanding these disease mechanisms given its role in elastic fiber assembly and maintenance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-LTBP4 antibody (ab222844)
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for LTBP4 (green) using ab222844 at 1/100 dilution in ICC/IF. Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) was used as secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LTBP4 antibody (ab222844)
Paraffin-embedded human kidney tissue stained for LTBP4 using ab222844 at 1/100 dilution in immunohistochemical analysis.
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