Anti-LTBR antibody [EPR26096-171] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-LTBR antibody [EPR26096-171] - BSA and Azide free (AB313562)

This data was developed using ab313561, the same antibody clone in a different buffer formulation. LTBR was immunoprecipitated from 0.35 mg Hela (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab313561 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313561 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Hela (human cervical adenocarcinoma epithelial cell) whole cell lysate 10 ug Lane 2 : Hela (human cervical adenocarcinoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab313561 in Hela whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 41 seconds Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Immunoprecipitation - Anti-LTBR antibody [EPR26096-171] (<a href='/en-us/products/primary-antibodies/ltbr-antibody-epr26096-171-ab313561'>ab313561</a>) at 1/30 dilution

All lanes:

Hela (human cervical adenocarcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 41s

• WB

Supplier Data

Western blot - Anti-LTBR antibody [EPR26096-171] - BSA and Azide free (AB313562)

This data was developed using ab313561, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysate was freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : 37 seconds

All lanes:

Western blot - Anti-LTBR antibody [EPR26096-171] (<a href='/en-us/products/primary-antibodies/ltbr-antibody-epr26096-171-ab313561'>ab313561</a>) at 1/1000 dilution

All lanes:

A549 (human lu carcinoma epithelial cell) while cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60 kDa

false

Exposure time: 37s

• WB

Supplier Data

Western blot - Anti-LTBR antibody [EPR26096-171] - BSA and Azide free (AB313562)

This data was developed using ab313561, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 48 seconds

All lanes:

Western blot - Anti-LTBR antibody [EPR26096-171] (<a href='/en-us/products/primary-antibodies/ltbr-antibody-epr26096-171-ab313561'>ab313561</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeti LTBR whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 60 kDa

false

Exposure time: 48s