Rabbit Recombinant Monoclonal LTBR antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt and reacts with Transfected cell lysate - Human, Human samples.
Images
![Western blot - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (AB309134), expandable thumbnail](https://content.abcam.com/products/images/ltbr-antibody-epr26097-53-bsa-and-azide-free-ab309134--western-blot-img409563.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (AB309134)")
![Immunocytochemistry/ Immunofluorescence - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (AB309134), expandable thumbnail](https://content.abcam.com/products/images/ltbr-antibody-epr26097-53-bsa-and-azide-free-ab309134--immunocytochemistry-immunofluorescence-img409562.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (AB309134)")
![Flow Cytometry - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (AB309134), expandable thumbnail](https://content.abcam.com/products/images/ltbr-antibody-epr26097-53-bsa-and-azide-free-ab309134--flow-cytometry-img409561.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (AB309134)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt | IHC-P | IP | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell lysate - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell lysate - Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT (PubMed:24248355). Activates NF-kappa-B signaling pathway upon stimulation with lymphotoxin (LTA(1)-LTB(2)) (PubMed:24248355). Promotes apoptosis via TRAF3 and TRAF5. May play a role in the development of lymphoid organs.
Alternative names
D12S370, TNFCR, TNFR3, TNFRSF3, LTBR, Tumor necrosis factor receptor superfamily member 3, Lymphotoxin-beta receptor, Tumor necrosis factor C receptor, Tumor necrosis factor receptor 2-related protein, Tumor necrosis factor receptor type III, TNF-RIII, TNFR-III
Recommended products
Rabbit Recombinant Monoclonal LTBR antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt and reacts with Transfected cell lysate - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26097-53
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LTBR (lymphotoxin beta receptor) also known as the TNFRSF3 is a significant protein that functions as a receptor for lymphotoxin and other related cytokines. The protein has a mass of around 62 kDa and is expressed in various tissues including lymphoid tissues and the epithelial cells of several organs. LTBR is present as a membrane-bound protein and helps mediate signaling that is important for cellular communication and function. Key interactions with ligands such as LTA and LIGHT activate signaling pathways which play roles in immune response.
- Biological function summary
LTBR coordinates multiple cellular processes especially in immune regulation and homeostasis. It forms a complex with cytokines that binds to its extracellular domain triggering downstream signaling cascades. These cascades lead to activation of nuclear factor kappa B (NF-kB) which controls gene transcription and promotes survival and differentiation of immune cells. The capability of LTBR to interact with other TNF receptors expands its influence in lymphoid organogenesis and maintenance especially in maintaining the integrity of the lymphoid system.
- Pathways
LTBR significantly participates in both the NF-kB and the non-canonical NF-kB pathways. These pathways are essential for regulating immune response and inflammation. Within these pathways LTBR interacts with other proteins such as TRAF3 and TRAF2 to mediate signaling processes. This arrangement allows for modulation of immune responses and is critical for recognizing and eliminating pathogens. Additionally the involvement of LTBR in immune system pathways aligns it with the functions of closely related proteins like TNF receptor superfamily members.
- Associated diseases and disorders
LTBR has been implicated in autoimmune diseases and cancer. In autoimmunity aberrant signaling through LTBR may lead to inappropriate immune responses and inflammation as seen in disorders like systemic lupus erythematosus. Moreover in oncology abnormalities in LTBR signaling can influence tumor microenvironments and support tumor cell survival. Throughout these disease contexts LTBR often interacts with other risk-associated proteins such as NFKB1 highlighting its integration in complex pathological processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (ab309134), expandable thumbnail](https://content.abcam.com/products/images/ltbr-antibody-epr26097-53-bsa-and-azide-free-ab309134--western-blot-img409563.jpg "Western blot - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (ab309134)")
Western blot - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (ab309134)
This data was developed using Anti-LTBR antibody [EPR26097-53] ab309133, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody detects transfected levels of LTBR in western blot.
In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.All lanes: Western blot - Anti-LTBR antibody [EPR26097-53] (Anti-LTBR antibody [EPR26097-53] ab309133) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containi a His-tag whole cell lysate
Lane 2: 293T cells transfected with a human LTBR expression vector containi a His-tag whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 75 kDa
Exposure time: 10s
![Immunocytochemistry/ Immunofluorescence - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (ab309134), expandable thumbnail](https://content.abcam.com/products/images/ltbr-antibody-epr26097-53-bsa-and-azide-free-ab309134--immunocytochemistry-immunofluorescence-img409562.jpg "Immunocytochemistry/ Immunofluorescence - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (ab309134)")
Immunocytochemistry/ Immunofluorescence - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (ab309134)
This data was developed using Anti-LTBR antibody [EPR26097-53] ab309133, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labeling LTBR with Anti-LTBR antibody [EPR26097-53] ab309133 at 1/50 (10.16 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous and cytoplasmic staining in HeLa cell line.Low expression: Jurkat (PMID: 10200501).The image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.![Flow Cytometry - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (ab309134), expandable thumbnail](https://content.abcam.com/products/images/ltbr-antibody-epr26097-53-bsa-and-azide-free-ab309134--flow-cytometry-img409561.jpg "Flow Cytometry - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (ab309134)")
Flow Cytometry - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (ab309134)
This data was developed using Anti-LTBR antibody [EPR26097-53] ab309133, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Left) / HeLa (human cervical adenocarcinoma epithelial cell, Right) cells labeling LTBR with Anti-LTBR antibody [EPR26097-53] ab309133 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Low expression: Jurkat (PMID: 10200501). Gated on viable cells.
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