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AB309134

Anti-LTBR antibody [EPR26097-53] - BSA and Azide free

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Rabbit Recombinant Monoclonal LTBR antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt and reacts with Transfected cell lysate - Human, Human samples.

View Alternative Names

D12S370, TNFCR, TNFR3, TNFRSF3, LTBR, Tumor necrosis factor receptor superfamily member 3, Lymphotoxin-beta receptor, Tumor necrosis factor C receptor, Tumor necrosis factor receptor 2-related protein, Tumor necrosis factor receptor type III, TNF-RIII, TNFR-III

3 Images
Flow Cytometry - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (AB309134)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (AB309134)

This data was developed using ab309133, the same antibody clone in a different buffer formulation. Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Left) / HeLa (human cervical adenocarcinoma epithelial cell, Right) cells labeling LTBR with ab309133 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Low expression : Jurkat (PMID : 10200501). Gated on viable cells.

Immunocytochemistry/ Immunofluorescence - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (AB309134)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (AB309134)

This data was developed using ab309133, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labeling LTBR with ab309133 at 1/50 (10.16 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous and cytoplasmic staining in HeLa cell line.Low expression : Jurkat (PMID : 10200501).The image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Western blot - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (AB309134)
  • WB

Supplier Data

Western blot - Anti-LTBR antibody [EPR26097-53] - BSA and Azide free (AB309134)

This data was developed using ab309133, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody detects transfected levels of LTBR in western blot. In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-LTBR antibody [EPR26097-53] (<a href='/en-us/products/primary-antibodies/ltbr-antibody-epr26097-53-ab309133'>ab309133</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containi a His-tag whole cell lysate

Lane 2:

293T cells transfected with a human LTBR expression vector containi a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa

false

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26097-53

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

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Proteins & Peptides

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LTBR (lymphotoxin beta receptor) also known as the TNFRSF3 is a significant protein that functions as a receptor for lymphotoxin and other related cytokines. The protein has a mass of around 62 kDa and is expressed in various tissues including lymphoid tissues and the epithelial cells of several organs. LTBR is present as a membrane-bound protein and helps mediate signaling that is important for cellular communication and function. Key interactions with ligands such as LTA and LIGHT activate signaling pathways which play roles in immune response.
Biological function summary

LTBR coordinates multiple cellular processes especially in immune regulation and homeostasis. It forms a complex with cytokines that binds to its extracellular domain triggering downstream signaling cascades. These cascades lead to activation of nuclear factor kappa B (NF-kB) which controls gene transcription and promotes survival and differentiation of immune cells. The capability of LTBR to interact with other TNF receptors expands its influence in lymphoid organogenesis and maintenance especially in maintaining the integrity of the lymphoid system.

Pathways

LTBR significantly participates in both the NF-kB and the non-canonical NF-kB pathways. These pathways are essential for regulating immune response and inflammation. Within these pathways LTBR interacts with other proteins such as TRAF3 and TRAF2 to mediate signaling processes. This arrangement allows for modulation of immune responses and is critical for recognizing and eliminating pathogens. Additionally the involvement of LTBR in immune system pathways aligns it with the functions of closely related proteins like TNF receptor superfamily members.

LTBR has been implicated in autoimmune diseases and cancer. In autoimmunity aberrant signaling through LTBR may lead to inappropriate immune responses and inflammation as seen in disorders like systemic lupus erythematosus. Moreover in oncology abnormalities in LTBR signaling can influence tumor microenvironments and support tumor cell survival. Throughout these disease contexts LTBR often interacts with other risk-associated proteins such as NFKB1 highlighting its integration in complex pathological processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the heterotrimeric lymphotoxin containing LTA and LTB, and for TNFS14/LIGHT (PubMed : 24248355). Activates NF-kappa-B signaling pathway upon stimulation with lymphotoxin (LTA(1)-LTB(2)) (PubMed : 24248355). Promotes apoptosis via TRAF3 and TRAF5. May play a role in the development of lymphoid organs.
See full target information LTBR
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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