Rabbit Recombinant Monoclonal LTK antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Western blot - Anti-LTK antibody [EPR5175(2)] - BSA and Azide free (AB248316), expandable thumbnail](https://content.abcam.com/products/images/ltk-antibody-epr51752-bsa-and-azide-free-ab248316--western-blot-img71001.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LTK antibody [EPR5175(2)] - BSA and Azide free (AB248316)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LTK antibody [EPR5175(2)] - BSA and Azide free (AB248316), expandable thumbnail](https://content.abcam.com/products/images/ltk-antibody-epr51752-bsa-and-azide-free-ab248316--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84334.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LTK antibody [EPR5175(2)] - BSA and Azide free (AB248316)")
![OI-RD Scanning - Anti-LTK antibody [EPR5175(2)] - BSA and Azide free (AB248316), expandable thumbnail](https://content.abcam.com/products/images/ltk-antibody-epr51752-bsa-and-azide-free-ab248316--oi-rd-scanning-img83893.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-LTK antibody [EPR5175(2)] - BSA and Azide free (AB248316)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Receptor with a tyrosine-protein kinase activity (PubMed:10445845, PubMed:20548102, PubMed:30061385). Following activation by ALKAL1 or ALKAL2 ligands at the cell surface, transduces an extracellular signal into an intracellular response (PubMed:30061385, PubMed:34646012). Ligand-binding to the extracellular domain induces tyrosine kinase activation, leading to activation of the mitogen-activated protein kinase (MAPK) pathway (PubMed:20548102). Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif (By similarity). The exact function of this protein is not known; studies with chimeric proteins demonstrate its ability to promote growth and specifically neurite outgrowth, and cell survival (PubMed:18849880, PubMed:9223670). Involved in regulation of the secretory pathway involving endoplasmic reticulum (ER) export sites (ERESs) and ER to Golgi transport (PubMed:20548102).
Alternative names
TYK1, LTK, Leukocyte tyrosine kinase receptor, Protein tyrosine kinase 1
Recommended products
Rabbit Recombinant Monoclonal LTK antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR5175(2)
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 7.6 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248316 is the carrier-free version of Anti-LTK antibody [EPR5175(2)] ab129155.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Leukocyte Tyrosine Kinase (LTK) also known as ALK-like receptor tyrosine kinase is a receptor tyrosine kinase with a molecular weight of approximately 94 kDa. It primarily gets expressed in immune-related tissues such as the thymus and spleen as well as in the nervous system. LTK consists of an extracellular ligand-binding domain a single transmembrane domain and an intracellular kinase domain. It plays role in signal transduction processes by transferring a phosphate group to tyrosine residues on target proteins which initiates downstream signaling pathways.
- Biological function summary
The LTK protein functions in the differentiation and development of cells particularly in the nervous and immune systems. It oligomerizes forming homodimers or heterodimers which activates its kinase activity. This activation promotes phosphorylation events that influence cellular processes like growth and survival. The receptor is not part of a larger complex but interacts directly with several adaptor proteins to exert its effects.
- Pathways
LTK is involved in the MAPK/ERK and PI3K/AKT pathways important for cell growth and survival signaling. The MAPK/ERK pathway includes related proteins such as RAS and RAF while the PI3K/AKT pathway interacts with proteins like PIK3CA and AKT1. These pathways converge on transcription factors and regulators that ultimately coordinate cellular responses to external signals.
- Associated diseases and disorders
LTK shows involvement in the pathogenesis of certain types of cancer and neurodevelopmental disorders like neuroblastoma and developmental delay. In neuroblastoma aberrant expression or mutations in LTK link to tumorigenic processes. It shares a connection with the ALK protein another receptor tyrosine kinase which also exhibits alterations in various cancers. Understanding these associations helps in identifying potential therapeutic targets for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-LTK antibody [EPR5175(2)] - BSA and Azide free (ab248316), expandable thumbnail](https://content.abcam.com/products/images/ltk-antibody-epr51752-bsa-and-azide-free-ab248316--western-blot-img71001.jpg "Western blot - Anti-LTK antibody [EPR5175(2)] - BSA and Azide free (ab248316)")
Western blot - Anti-LTK antibody [EPR5175(2)] - BSA and Azide free (ab248316)
This data was developed using Anti-LTK antibody [EPR5175(2)] ab129155, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-LTK antibody [EPR5175(2)] (Anti-LTK antibody [EPR5175(2)] ab129155) at 1/1000 dilution
Lane 1: Raji cell lysate at 10 µg
Lane 2: Human tonsil lysate at 10 µg
Lane 3: Human spleen lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 92 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LTK antibody [EPR5175(2)] - BSA and Azide free (ab248316), expandable thumbnail](https://content.abcam.com/products/images/ltk-antibody-epr51752-bsa-and-azide-free-ab248316--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84334.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LTK antibody [EPR5175(2)] - BSA and Azide free (ab248316)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LTK antibody [EPR5175(2)] - BSA and Azide free (ab248316)
This data was developed using Anti-LTK antibody [EPR5175(2)] ab129155, the same antibody clone in a different buffer formulation.Anti-LTK antibody [EPR5175(2)] ab129155, at a 1/250 dilution, staining LTK in paraffin embedded Human colon tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![OI-RD Scanning - Anti-LTK antibody [EPR5175(2)] - BSA and Azide free (ab248316), expandable thumbnail](https://content.abcam.com/products/images/ltk-antibody-epr51752-bsa-and-azide-free-ab248316--oi-rd-scanning-img83893.jpg "OI-RD Scanning - Anti-LTK antibody [EPR5175(2)] - BSA and Azide free (ab248316)")
OI-RD Scanning - Anti-LTK antibody [EPR5175(2)] - BSA and Azide free (ab248316)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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