AB28484

Anti-Lubricin/MSF antibody

Name: Anti-Lubricin/MSF antibody (ab28484) | Abcam
Brand: Abcam Limited
SKU: AB28484
Price: 515 USD
Availability: InStock
Rating: 5 (7 reviews)

(7 Reviews)

(74 Publications)

Anti-Lubricin/MSF antibody (ab28484) is a rabbit polyclonal antibody detecting Lubricin/MSF in Western Blot, IHC-P, ICC/IF. Suitable for African green monkey, Human, Mouse.

- Over 50 publications
- Trusted since 2006

View Alternative Names

MSF, SZP, PRG4, Proteoglycan 4, Lubricin, Megakaryocyte-stimulating factor, Superficial zone proteoglycan

1 Images

Supplier Data

Western blot - Anti-Lubricin/MSF antibody (AB28484)

Primary antibody was diluted in antibody diluent using a Fast Western Blot kit and followed by the optimized HRP reagent. Chemiluminescent detection was performed using SuperSignal West Dura.

All lanes:

Western blot - Anti-Lubricin/MSF antibody (ab28484) at 2 µg/mL

All lanes:

non-reduced human synovial fluid at 3 µg

Predicted band size: 151 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, African green monkey, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human PRG4 aa 1350-1400. The exact immunogen used to generate this antibody is proprietary information.

Q92954

Specificity

ab28484 recognises Lubricin/MSF.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Lubricin/MSF antibody (ab28484) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in African green monkey, Human, Mouse samples.

What is the molecular weight of Lubricin/MSF?
Anti-Lubricin/MSF (ab28484) specifically detects a band for Lubricin/MSF (UniProt: Q92954) at a molecular weight of 156kDa.

Trusted by the scientific community
Anti-Lubricin/MSF (ab28484) was first used in a scientific publication in 2006 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-Lubricin/MSF (ab28484) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.. Isoform F plays a role as a growth factor acting on the primitive cells of both hematopoietic and endothelial cell lineages.

See full target information PRG4

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Publications (74)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical medicine 14: PubMed40869671

2025

Insights into Vascular Changes in Hip Degenerative Disorders: An Observational Study.

Applications

Unspecified application

Species

Unspecified reactive species

Riana Maria Huzum,Bogdan Huzum,Marius Valeriu Hinganu,Ludmila Lozneanu,Fabian Cezar Lupu,Delia Hinganu

Diagnostics (Basel, Switzerland) 15: PubMed40804896

2025

Immunohistochemical and Ultrastructural Study of the Degenerative Processes of the Hip Joint Capsule and Acetabular Labrum.

Applications

Unspecified application

Species

Unspecified reactive species

Riana Maria Huzum,Bogdan Huzum,Marius Valeriu Hînganu,Ludmila Lozneanu,Fabian Cezar Lupu,Delia Hînganu

iScience 27:110537 PubMed39193188

2024

Time- and cell-specific activation of BMP signaling restrains chondrocyte hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen J Gadomski,Byron W H Mui,Raphael Gorodetsky,Sriram S Paravastu,Joseph Featherall,Li Li,Abigail Haffey,Jae-Chun Kim,Sergei A Kuznetsov,Kathryn Futrega,Astar Lazmi-Hailu,Randall K Merling,Daniel Martin,Andrew W McCaskie,Pamela G Robey

Molecular biology reports 51:862 PubMed39073659

2024

Effects of different combinations of mechanical loading intensity, duration, and frequency on the articular cartilage in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshio Wakimoto,Yasushi Miura,Shota Inoue,Masato Nomura,Hideki Moriyama

Stem cell research & therapy 15:209 PubMed39020442

2024

Single-cell sequencing of facial adipose tissue unveils FKBP5 as a therapeutic target for facial infiltrating lipomatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hongrui Chen,Bin Sun,Shih-Jen Chang,Zhang Yu,Yajing Qiu,Chen Hua,Xiaoxi Lin

Molecular therapy : the journal of the American Society of Gene Therapy 32:2549-2562 PubMed38879753

2024

CRISPR-mediated Sox9 activation and RelA inhibition enhance cell therapy for osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Zhao,Yumei Lai,Hongli Jiao,Jun Li,Ke Lu,Jian Huang

Bone research 12:24 PubMed38594260

2024

Sorafenib inhibits ossification of the posterior longitudinal ligament by blocking LOXL2-mediated vascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Longqing Wang,Wenhao Jiang,Siyuan Zhao,Dong Xie,Qing Chen,Qi Zhao,Hao Wu,Jian Luo,Lili Yang

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 39:161-176 PubMed38477740

2024

Synovium and infrapatellar fat pad share common mesenchymal progenitors and undergo coordinated changes in osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Li,Tao Gui,Lutian Yao,Hanli Guo,Yu-Lieh Lin,Jiawei Lu,Michael Duffy,Miltiadis Zgonis,Robert Mauck,Nathaniel Dyment,Yejia Zhang,Carla Scanzello,Patrick Seale,Ling Qin

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 42:1841-1851 PubMed38433390

2024

Regenerative capacity of human pluripotent stem cell-derived articular chondrocytes in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Rosanne M Raftery,Steven K Pregizer,Sophia Kocher,April M Craft

Nature communications 14:6909 PubMed37907525

2023

Loss of Grem1-lineage chondrogenic progenitor cells causes osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Q Ng,Toghrul H Jafarov,Christopher B Little,Tongtong Wang,Abdullah M Ali,Yan Ma,Georgette A Radford,Laura Vrbanac,Mari Ichinose,Samuel Whittle,David J Hunter,Tamsin R M Lannagan,Nobumi Suzuki,Jarrad M Goyne,Hiroki Kobayashi,Timothy C Wang,David R Haynes,Danijela Menicanin,Stan Gronthos,Daniel L Worthley,Susan L Woods,Siddhartha Mukherjee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lubricin/MSF antibody

Western blot - Anti-Lubricin/MSF antibody (AB28484)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (74)

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