Rabbit Recombinant Monoclonal Lubricin/MSF antibody. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-Lubricin/MSF antibody [EPR27164-57] (AB322552), expandable thumbnail](https://content.abcam.com/products/images/lubricin-msf-antibody-epr27164-57-ab322552--western-blot-img440985.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lubricin/MSF antibody [EPR27164-57] (AB322552)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Not recommended |
| Mouse | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Target data
Function
Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface. Isoform F plays a role as a growth factor acting on the primitive cells of both hematopoietic and endothelial cell lineages.
Alternative names
MSF, SZP, PRG4, Proteoglycan 4, Lubricin, Megakaryocyte-stimulating factor, Superficial zone proteoglycan
Rabbit Recombinant Monoclonal Lubricin/MSF antibody. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR27164-57
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-Lubricin/MSF antibody [EPR27164-57] (ab322552), expandable thumbnail](https://content.abcam.com/products/images/lubricin-msf-antibody-epr27164-57-ab322552--western-blot-img440985.jpg "Western blot - Anti-Lubricin/MSF antibody [EPR27164-57] (ab322552)")
Western blot - Anti-Lubricin/MSF antibody [EPR27164-57] (ab322552)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: cerebellum, placenta (PMID:10545950).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:10545950, PMID: 25187573, PMID: 25642942).
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
Exposure time: Lane 1: 26 seconds, lanes 2-5: 180 seconds
All lanes: Western blot - Anti-Lubricin/MSF antibody [EPR27164-57] (ab322552) at 1/1000 dilution
Lane 1: Human synovial fluid lysate at 10 µL
Lane 2: Human cerebellum tissues lysate at 20 µg
Lane 3: Human liver cancer tissues lysate at 20 µg
Lane 4: Human liver tissues lysate at 20 µg
Lane 5: Human placenta tissues lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 300-460 kDa, 124 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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