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AB322552

Anti-Lubricin/MSF antibody [EPR27164-57]

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Rabbit Recombinant Monoclonal Lubricin/MSF antibody. Suitable for WB and reacts with Human samples.

View Alternative Names

MSF, SZP, PRG4, Proteoglycan 4, Lubricin, Megakaryocyte-stimulating factor, Superficial zone proteoglycan

1 Images
Western blot - Anti-Lubricin/MSF antibody [EPR27164-57] (AB322552)
  • WB

Supplier Data

Western blot - Anti-Lubricin/MSF antibody [EPR27164-57] (AB322552)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : cerebellum, placenta (PMID : 10545950).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 10545950, PMID : 25187573, PMID : 25642942).

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

Exposure time : Lane 1 : 26 seconds, lanes 2-5 : 180 seconds

All lanes:

Western blot - Anti-Lubricin/MSF antibody [EPR27164-57] (ab322552) at 1/1000 dilution

Lane 1:

Human synovial fluid lysate  at 10 µL

Lane 2:

Human cerebellum tissues lysate at 20 µg

Lane 3:

Human liver cancer tissues lysate at 20 µg

Lane 4:

Human liver tissues lysate at 20 µg

Lane 5:

Human placenta tissues lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 300-460 kDa,124 kDa

false

  • Carrier free

    Anti-Lubricin/MSF antibody [EPR27164-57] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27164-57

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.. Isoform F plays a role as a growth factor acting on the primitive cells of both hematopoietic and endothelial cell lineages.
See full target information PRG4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com