Rabbit Recombinant Monoclonal Lumican antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Western blot - Anti-Lumican antibody [EPR22510-47] (AB252924), expandable thumbnail](https://content.abcam.com/products/images/lumican-antibody-epr22510-47-ab252924--western-blot-img56214.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lumican antibody [EPR22510-47] (AB252924)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (AB252924), expandable thumbnail](https://content.abcam.com/products/images/lumican-antibody-epr22510-47-ab252924--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img30068.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (AB252924)")
![Western blot - Anti-Lumican antibody [EPR22510-47] (AB252924), expandable thumbnail](https://content.abcam.com/products/images/lumican-antibody-epr22510-47-ab252924--western-blot-img85253.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lumican antibody [EPR22510-47] (AB252924)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (AB252924), expandable thumbnail](https://content.abcam.com/products/images/lumican-antibody-epr22510-47-ab252924--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8943.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (AB252924)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (AB252924), expandable thumbnail](https://content.abcam.com/products/images/lumican-antibody-epr22510-47-ab252924--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84718.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (AB252924)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Tested | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Tested | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Alternative names
LDC, SLRR2D, LUM, Lumican, Keratan sulfate proteoglycan lumican, KSPG lumican
Recommended products
Rabbit Recombinant Monoclonal Lumican antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR22510-47
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Lumican antibody [EPR22510-47] (ab252924), expandable thumbnail](https://content.abcam.com/products/images/lumican-antibody-epr22510-47-ab252924--western-blot-img56214.jpg "Western blot - Anti-Lumican antibody [EPR22510-47] (ab252924)")
Western blot - Anti-Lumican antibody [EPR22510-47] (ab252924)
Exposure timeLane 1: 81 seconds
Lane 2: 15 seconds
Lane 3: 3 minutes
Lanes 4-5: 26 secondsBlocking buffer and concentration 5% NFDM/TBST
Diluting buffer and concentration 5% NFDM/TBSTThe molecular weight observed is consistent with what has been described in the literature (PMID:28459201,22266188,18949819)
All lanes: Western blot - Anti-Lumican antibody [EPR22510-47] (ab252924) at 1/1000 dilution
Lane 1: Human colon tissue lysate at 10 µg
Lane 2: Human skin tissue lysate at 10 µg
Lane 3: Human placenta tissue lysate at 10 µg
Lane 4: Mouse skin tissue lysate at 10 µg
Lane 5: Rat skin tissue lysate at 10 µg
SecondaryLanes 1 - 3: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Lanes 4 - 5: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 50 kDa, 70 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (ab252924), expandable thumbnail](https://content.abcam.com/products/images/lumican-antibody-epr22510-47-ab252924--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img30068.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (ab252924)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (ab252924)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Lumican with ab252924 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stroma cells of human colon (PMID:17089042, 23399832) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Western blot - Anti-Lumican antibody [EPR22510-47] (ab252924), expandable thumbnail](https://content.abcam.com/products/images/lumican-antibody-epr22510-47-ab252924--western-blot-img85253.jpg "Western blot - Anti-Lumican antibody [EPR22510-47] (ab252924)")
Western blot - Anti-Lumican antibody [EPR22510-47] (ab252924)
The molecular weight observed is consistent with what has been described in the literature (PMID:28459201,22266188,18949819).
This blot was developed using a higher sensitivity ECL substrate.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Lumican antibody [EPR22510-47] (ab252924) at 1/1000 dilution
Lane 1: Human kidney tissue lysate at 20 µg
Lane 2: Saos-2 (human osteosarcoma epithelial), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (ab252924), expandable thumbnail](https://content.abcam.com/products/images/lumican-antibody-epr22510-47-ab252924--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8943.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (ab252924)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (ab252924)
Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling Lumican with ab252924 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stroma cells of mouse skin (PMID:17089042) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (ab252924), expandable thumbnail](https://content.abcam.com/products/images/lumican-antibody-epr22510-47-ab252924--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84718.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (ab252924)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (ab252924)
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Lumican with ab252924 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stroma cells of rat colon (PMID:17089042, 23399832) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
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