Anti-Lumican antibody [EPR22510-47]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (AB252924)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Lumican with ab252924 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stroma cells of human colon (PMID : 17089042, 23399832) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (AB252924)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling Lumican with ab252924 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stroma cells of mouse skin (PMID : 17089042) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR22510-47] (AB252924)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Lumican with ab252924 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stroma cells of rat colon (PMID : 17089042, 23399832) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• WB

Unknown

Western blot - Anti-Lumican antibody [EPR22510-47] (AB252924)

The molecular weight observed is consistent with what has been described in the literature (PMID : 28459201,22266188,18949819).

This blot was developed using a higher sensitivity ECL substrate.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Lumican antibody [EPR22510-47] (ab252924) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

Saos-2 (human osteosarcoma epithelial), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 38 kDa

false

Exposure time: 3min

• WB

Lab

Western blot - Anti-Lumican antibody [EPR22510-47] (AB252924)

Exposure time

Lane 1 : 81 seconds
Lane 2 : 15 seconds
Lane 3 : 3 minutes
Lanes 4-5 : 26 seconds

Blocking buffer and concentration 5% NFDM/TBST
Diluting buffer and concentration 5% NFDM/TBST

The molecular weight observed is consistent with what has been described in the literature (PMID : 28459201,22266188,18949819)

All lanes:

Western blot - Anti-Lumican antibody [EPR22510-47] (ab252924) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 10 µg

Lane 2:

Human skin tissue lysate at 10 µg

Lane 3:

Human placenta tissue lysate at 10 µg

Lane 4:

Mouse skin tissue lysate at 10 µg

Lane 5:

Rat skin tissue lysate at 10 µg

Secondary

Lanes 1 - 3:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Lanes 4 - 5:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 38 kDa

Observed band size: 50 kDa,70 kDa

false