Anti-Lumican antibody [EPR22511-63] - BSA and Azide free

• IP

Unknown

Immunoprecipitation - Anti-Lumican antibody [EPR22511-63] - BSA and Azide free (AB254995)

Lumican was immunoprecipitated from 0.35 mg of mouse skin tissue lysate with ab252925 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab252925 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1 : Mouse skin tissue lysate 10 μg (Input).
Lane 2 : ab252925 IP in mouse skin tissue lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab252925 in mouse skin tissue lysate lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252925).

All lanes:

Immunoprecipitation - Anti-Lumican antibody [EPR22511-63] (<a href='/en-us/products/primary-antibodies/lumican-antibody-epr22511-63-ab252925'>ab252925</a>)

Predicted band size: 38 kDa

Observed band size: 50 kDa,70 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-Lumican antibody [EPR22511-63] - BSA and Azide free (AB254995)

Lumican was immunoprecipitated from 0.35 mg of human skin tissue lysate with ab252925 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab252925 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1 : Human skin tissue lysate 10 μg (Input).
Lane 2 : ab252925 IP in human skin tissue lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab252925 in human skin tissue lysate lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 15 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252925).

All lanes:

Immunoprecipitation - Anti-Lumican antibody [EPR22511-63] (<a href='/en-us/products/primary-antibodies/lumican-antibody-epr22511-63-ab252925'>ab252925</a>)

Predicted band size: 38 kDa

Observed band size: 50 kDa,70 kDa

false

• WB

Supplier Data

Western blot - Anti-Lumican antibody [EPR22511-63] - BSA and Azide free (AB254995)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times.

Lane 1 : 15 seconds; Lane 2 : 7.75 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 22266188).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252925).

All lanes:

Western blot - Anti-Lumican antibody [EPR22511-63] (<a href='/en-us/products/primary-antibodies/lumican-antibody-epr22511-63-ab252925'>ab252925</a>) at 1/1000 dilution

Lane 1:

Mouse skin tissue lysate at 20 µg

Lane 2:

Rat skin tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 38 kDa

false

• WB

Supplier Data

Western blot - Anti-Lumican antibody [EPR22511-63] - BSA and Azide free (AB254995)

Blocking and dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 28459201,22266188).

This blot was developed using a higher sensitivity ECL substrate.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252925).

All lanes:

Western blot - Anti-Lumican antibody [EPR22511-63] (<a href='/en-us/products/primary-antibodies/lumican-antibody-epr22511-63-ab252925'>ab252925</a>) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

Saos-2 (human osteosarcoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 38 kDa

Observed band size: 50 kDa,70 kDa

true

Exposure time: 3min

• WB

Unknown

Western blot - Anti-Lumican antibody [EPR22511-63] - BSA and Azide free (AB254995)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times.

Lane 1 : 48 seconds; Lane 2 : 7.75 seconds; Lane 3 : 37 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 28459201,22266188).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252925).

All lanes:

Western blot - Anti-Lumican antibody [EPR22511-63] (<a href='/en-us/products/primary-antibodies/lumican-antibody-epr22511-63-ab252925'>ab252925</a>) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 20 µg

Lane 2:

Human skin tissue lysate at 20 µg

Lane 3:

Human placenta tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 38 kDa

Observed band size: 50 kDa,70 kDa

false