Anti-Lumican antibody [EPR8898(2)]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Lumican antibody [EPR8898(2)] (AB168348)

Intracellular flow cytometrical analysis of permeabilized 293T cells with ab168348 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR8898(2)] (AB168348)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Lumican with ab168348 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Lumican antibody [EPR8898(2)] (AB168348)

Immunofluorescent analysis of Saos-2 cells labeling Lumican with ab168348 at 1/250.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lumican antibody [EPR8898(2)] (AB168348)

Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Lumican with ab168348 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Lumican antibody [EPR8898(2)] (AB168348)

Flow cytometry overlay histogram showing HeLa cells stained with ab168348 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 mins. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab168348) (1x 10⁶ in 100μl at 0.2μg/ml (1/10,000 dilution)) for 30 mins at 22°C.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed was incubated at 1/2000 dilution for 30 mins at 22°C

Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

This antibody gave a positive signal in HeLa Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 mins under the same conditions.

• WB

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Western blot - Anti-Lumican antibody [EPR8898(2)] (AB168348)

All lanes:

Western blot - Anti-Lumican antibody [EPR8898(2)] (ab168348) at 1/1000 dilution

Lane 1:

Saos-2 lysate at 10 µg

Lane 2:

Fetal skin lysate at 10 µg

Lane 3:

Fetal heart lysate at 10 µg

Lane 4:

Jurkat lysate at 10 µg

Lane 5:

Y79 lysate at 10 µg

Lane 6:

293T lysate at 10 µg

Predicted band size: 38 kDa

Observed band size: 50 kDa

false

• WB

CiteAb

Western blot - Anti-Lumican antibody [EPR8898(2)] (AB168348)

Lumican western blot using anti-Lumican antibody [EPR8898(2)] ab168348. Publication image and figure legend from Simões, R. S., Soares, J. M., et al., 2017, J Ovarian Res, PubMed 28789706.

ab168348 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab168348 please see the product overview.

Western blot results for decorin, lumican, biglycan, and fibromodulin expression in the endometrium of CONTROL and PCOS women

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