Rabbit Recombinant Monoclonal Lunatic Fringe antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Human samples. Cited in 2 publications.
Images
![Western blot - Anti-Lunatic Fringe antibody [EPR10391(B)] (AB151699), expandable thumbnail](https://content.abcam.com/products/images/lunatic-fringe-antibody-epr10391b-ab151699--western-blot-img68669.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Lunatic Fringe antibody [EPR10391(B)] (AB151699)")
![Flow Cytometry (Intracellular) - Anti-Lunatic Fringe antibody [EPR10391(B)] (AB151699), expandable thumbnail](https://content.abcam.com/products/images/lunatic-fringe-antibody-epr10391b-ab151699--flow-cytometry-intracellular-img2420.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Lunatic Fringe antibody [EPR10391(B)] (AB151699)")
Publications
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- Turkish journal of biology = Turk biyoloji dergisi 47:186-1982023tsRNA-15797-modified BMSC-derived exosomes mediate LFNG to induce angiogenesis in osteonecrosis of the femoral head.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShanhong Fang,Mengqiang You,Jie Wei,Peng ChenPubMed 37529417
- Journal of neurosurgery 126:249-2592016Bioinformatic analyses reveal a distinct Notch activation induced by STAT3 phosphorylation in the mesenchymal subtype of glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen Cheng,Chuanbao Zhang,Xiufang Ren,Yang Jiang,Sheng Han,Yang Liu,Jinquan Cai,Mingyang Li,Kuanyu Wang,Yanwei Liu,Huimin Hu,Qingbin Li,Pei Yang,Zhaoshi Bao,Anhua WuPubMed 26967788
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Expected | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Predicted | Not recommended | Predicted |
| Rat | Not recommended | Not recommended | Tested | Not recommended | Expected |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Modulates NOTCH1 activity by modifying O-fucose residues at specific EGF-like domains resulting in inhibition of NOTCH1 activation by JAG1 and enhancement of NOTCH1 activation by DLL1 via an increase in its binding to DLL1 (By similarity). Decreases the binding of JAG1 to NOTCH2 but not that of DLL1 (PubMed:11346656). Essential mediator of somite segmentation and patterning (By similarity).
Alternative names
O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase, LFNG
Recommended products
Rabbit Recombinant Monoclonal Lunatic Fringe antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR10391(B)
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699), expandable thumbnail](https://content.abcam.com/products/images/lunatic-fringe-antibody-epr10391b-ab151699--western-blot-img68669.jpg "Western blot - Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699)")
Western blot - Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699)
All lanes: Western blot - Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699) at 1/1000 dilution
Lane 1: PC12 cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: K562 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 42 kDa
![Flow Cytometry (Intracellular) - Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699), expandable thumbnail](https://content.abcam.com/products/images/lunatic-fringe-antibody-epr10391b-ab151699--flow-cytometry-intracellular-img2420.jpg "Flow Cytometry (Intracellular) - Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699)")
Flow Cytometry (Intracellular) - Anti-Lunatic Fringe antibody [EPR10391(B)] (ab151699)
Intracellular flow cytometric analysis of permeabilized K562 cells labeling Lunatic Fringe with ab151699 at 1/10 dilution (red) compared to a nonspecific control antibody (green).
Downloads
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