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AB3585

Anti-LXR alpha antibody

4

(3 Reviews)

|

(25 Publications)

Rabbit Polyclonal LXR alpha antibody. Suitable for ICC, EMSA, IHC-FoFr, ICC/IF, Gel Shift Assay and reacts with Human, Chinese hamster, Mouse, Rat samples. Cited in 25 publications. Immunogen corresponding to Synthetic Peptide within Human NR1H3 aa 300-350.

View Alternative Names

LXRA, NR1H3, Oxysterols receptor LXR-alpha, Liver X receptor alpha, Nuclear receptor subfamily 1 group H member 3

2 Images
Immunocytochemistry - Anti-LXR alpha antibody (AB3585)
  • ICC

Supplier Data

Immunocytochemistry - Anti-LXR alpha antibody (AB3585)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.

Immunocytochemistry - Anti-LXR alpha antibody (AB3585)
  • ICC

Supplier Data

Immunocytochemistry - Anti-LXR alpha antibody (AB3585)

Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labeling LXR alpha (green) with ab3585 at 1/300. F-Actin staining with Phalloidin (red) and nuclei with DAPI or Hoechst (blue). Cells were fixed with formalin, permeablized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody overnight at 4°C. A DyLight-conjugated secondary antibody was used. 60X magnification. Left - negative control.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat, Chinese hamster

Applications

EMSA, ICC, IHC-FoFr, Gel Shift Assay, ICC/IF

applications

Immunogen

Synthetic Peptide within Human NR1H3 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

Q13133

Specificity

Detects recombinant Human LXR alpha. This antibody does not detect recombinant Human LXR beta.

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LXR alpha also known as NR1H3 is a nuclear receptor protein involved in the regulation of lipid metabolism and inflammation. It has a molecular mass of approximately 55 kDa. Expressed mainly in the liver adipose tissue macrophages and intestines LXR alpha acts as a transcription factor controlling the expression of genes important for cholesterol homeostasis. It binds to DNA at specific LXR response elements usually forming heterodimers with the retinoid X receptor (RXR).
Biological function summary

LXR alpha plays a critical role in lipid metabolism and cholesterol efflux. It is part of a larger LXR and CO complex which contributes to the regulation of genes that manage cholesterol absorption transport and excretion. For instance LXR activation increases the expression of ATP-binding cassette transporters such as ABCA1 and ABCG1 which are essential for transporting cholesterol out of cells and facilitating reverse cholesterol transport.

Pathways

The function of LXR alpha is significant in both the cholesterol catabolism pathway and the inflammatory response pathway. Within these pathways it upregulates genes involved in cholesterol catabolism providing a mechanism to prevent excess accumulation in cells and tissues. LXR alpha interacts closely with other nuclear receptors and proteins like PPARs (peroxisome proliferator-activated receptors) highlighting its role in lipid signaling networks.

LXR alpha is linked to atherosclerosis and Alzheimer’s disease. Its ability to regulate cholesterol transport and immune function demonstrates involvement in the pathogenesis of atherosclerosis by influencing macrophage-derived foam cell formation. Additionally improper cholesterol metabolism is associated with Alzheimer’s disease where LXR alpha's interaction with amyloid precursor protein (APP) may influence disease progression connecting cholesterol dysfunction with neurodegenerative processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed : 19481530, PubMed : 25661920, PubMed : 37478846). Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES (PubMed : 37478846). LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides (By similarity). Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8 (PubMed : 19481530). Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity). Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis (By similarity). Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators (By similarity).
See full target information NR1H3
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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in veterinary science 12:1654799 PubMed40874205

2025

Dietary fiber enhances milk yield in plateau dairy cows via activation of the rumen microbiota-mammary gland axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Li,Dongxu Wen,Ziwei Zhou,Quji Suolang,Wangmu Siwang,Lamu Kangji,Tuoxian Tang,Zhenjiang Liu,Yachun Wang

Scientific reports 15:11462 PubMed40181151

2025

Cholesterol metabolism and neuroinflammatory changes in a non-human primate spinal nerve ligation model.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroshi Yamane,Suguru Koyama,Takayuki Komatsu,Tomoya Tanaka,Riyu Koguchi,Haruhisa Watanabe,Mai Nishiura,Satoru Yoshikawa,Tadahiro Iimura

The Journal of experimental medicine 222: PubMed39853307

2025

RNase T2 deficiency promotes TLR13-dependent replenishment of tissue-protective Kupffer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ryota Sato,Kaiwen Liu,Takuma Shibata,Katsuaki Hoshino,Kiyoshi Yamaguchi,Toru Miyazaki,Ryosuke Hiranuma,Ryutaro Fukui,Yuji Motoi,Yuri Fukuda-Ohta,Yun Zhang,Tatjana Reuter,Yuko Ishida,Toshikazu Kondo,Tomoki Chiba,Hiroshi Asahara,Masato Taoka,Yoshio Yamauchi,Toshiaki Isobe,Tsuneyasu Kaisho,Yoichi Furukawa,Eicke Latz,Kohta Nakatani,Yoshihiro Izumi,Yunzhong Nie,Hideki Taniguchi,Kensuke Miyake

Biochimica et biophysica acta. Molecular basis of disease 1871:167573 PubMed39547517

2024

Physiological activation of liver X receptor provides protection against ocular inflammation in uveitic glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun Hee Ju,Jiyoung Lee,Seon-Kyu Kim,Seon-Young Kim,Jin-Hyun Ahn,Nikolai P Skiba,Ponugoti V Rao,Jin A Choi

BMC complementary medicine and therapies 23:445 PubMed38066464

2023

Danlou Recipe promotes cholesterol efflux in macrophages RAW264.7 and reverses cholesterol transport in mice with hyperlipidemia induced by P407.

Applications

Unspecified application

Species

Unspecified reactive species

Wenrun Han,Dandan Zhang,Peng Zhang,Qianqian Tao,Xiaoli Du,Chunquan Yu,Pengzhi Dong,Yan Zhu

Diabetologia 64:1674-1689 PubMed33770194

2021

Fasting and fasting-mimicking treatment activate SIRT1/LXRα and alleviate diabetes-induced systemic and microvascular dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra S Hammer,Cristiano P Vieira,Delaney McFarland,Maximilian Sandler,Yan Levitsky,Tim F Dorweiler,Todd A Lydic,Bright Asare-Bediako,Yvonne Adu-Agyeiwaah,Micheli S Sielski,Mariana Dupont,Ana Leda Longhini,Sergio Li Calzi,Dibyendu Chakraborty,Gail M Seigel,Denis A Proshlyakov,Maria B Grant,Julia V Busik

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 17:1253-1270 PubMed32297184

2020

Activation of LXRβ Signaling in the Amygdala Confers Anxiolytic Effects Through Rebalancing Excitatory and Inhibitory Neurotransmission upon Acute Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Yu,Lu Wang,Le Yang,Yan-Jiao Li,Min Wang,Chen Qiu,Qi Yang,Xu-Bo Li,Yun-Long Huang,Rui Liu,Yu-Mei Wu

Journal of cellular and molecular medicine 24:3384-3398 PubMed31981312

2020

The marine-derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRα and PPARα.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Li,Shu-Mei Hu,Xiao-Yan Pang,Jun-Feng Wang,Jia-Yu Yin,Fa-Hui Li,Jin Wang,Xiao-Qian Yang,Bin Xia,Yong-Hong Liu,Wei-Guo Song,Shou-Dong Guo

Food & function 10:5124-5139 PubMed31364648

2019

The fucoidan from the brown seaweed Ascophyllum nodosum ameliorates atherosclerosis in apolipoprotein E-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayu Yin,Jin Wang,Fahui Li,Zixun Yang,Xiaoqian Yang,Wanli Sun,Bin Xia,Ting Li,Weiguo Song,Shoudong Guo

Viruses 11: PubMed31242674

2019

Establishment and Comparison of Pathogenicity and Related Neurotropism in Two Age Groups of Immune Competent Mice, C57BL/6J Using an Indian Isolate of Chikungunya Virus (CHIKV).

Applications

Unspecified application

Species

Unspecified reactive species

Jaspreet Jain,Vimal Narayanan,Ankit Kumar,Jatin Shrinet,Priyanshu Srivastava,Shivam Chaturvedi,Sujatha Sunil
View all publications
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