AB176323

Anti-LXR alpha antibody [EPR6508(N)]

Name: Anti-LXR alpha antibody [EPR6508(N)] (ab176323) | Abcam
Brand: Abcam Limited
SKU: AB176323
Price: 270 USD
Availability: InStock
Rating: 4 (3 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(3 Reviews)

(73 Publications)

Rabbit Recombinant Monoclonal LXR alpha antibody. Suitable for WB and reacts with Human, Transfected cell line - Human samples. Cited in 73 publications.

View Alternative Names

LXRA, NR1H3, Oxysterols receptor LXR-alpha, Liver X receptor alpha, Nuclear receptor subfamily 1 group H member 3

3 Images

Lab

Western blot - Anti-LXR alpha antibody [EPR6508(N)] (AB176323)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM/TBST ab176323 does not cross activate with human LXR beta. ab213204 was used as loading control.

All lanes:

Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323) at 1/1000 dilution

Lane 1:

HEK 293 transfected with blank vectors, whole cell lysate at 20 µg

Lane 2:

HEK 293 transfected with His tagged human LXR alpha full length expression vectors, whole cell lysate at 20 µg

Lane 3:

HEK 293 transfected with His tagged human LXR beta full length expression vectors, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Exposure time: 3s

Lab

Western blot - Anti-LXR alpha antibody [EPR6508(N)] (AB176323)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM/TBST ab176323 detects a higher band in nuclear fraction than in non-nuclear fraction, which might be the correct band of interest. ab176842 and ab181602 were used as loading controls.

All lanes:

Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) cells non-nuclear fraction at 20 µg

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) cells nuclear fraction at 20 µg

Lane 3:

SH-SY5Y (Human neuroblastoma epithelial cell) cells non-nuclear fraction at 20 µg

Lane 4:

SH-SY5Y (Human neuroblastoma epithelial cell) cells nuclear fraction at 20 µg

Lane 5:

IMR-32 (Human neuroblastoma neuroblast) cells non-nuclear fraction at 20 µg

Lane 6:

IMR-32 (Human neuroblastoma neuroblast) cells nuclear fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Exposure time: 7s

Lab

Western blot - Anti-LXR alpha antibody [EPR6508(N)] (AB176323)

All lanes:

Western blot - Anti-LXR alpha antibody [EPR6508(N)] (ab176323) at 1/1000 dilution

Lane 1:

HEK 293 transfected with blank vectors, whole cell lysate at 20 µg

Lane 2:

HEK 293 transfected with blank vectors, non-nuclear fraction at 20 µg

Lane 3:

HEK 293 transfected with blank vectors, nuclear fraction at 20 µg

Lane 4:

HEK 293 transfected with His tagged human LXR alpha full length expression vectors, non-nuclear fraction at 20 µg

Lane 5:

HEK 293 transfected with His tagged human LXR alpha full length expression vectors, nuclear fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Exposure time: 10s

Carrier free

Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free
HRP

HRP Anti-LXR alpha antibody [EPR6508(N)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6508(N)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Sheep": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell line - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LXR alpha also known as NR1H3 is a nuclear receptor protein involved in the regulation of lipid metabolism and inflammation. It has a molecular mass of approximately 55 kDa. Expressed mainly in the liver adipose tissue macrophages and intestines LXR alpha acts as a transcription factor controlling the expression of genes important for cholesterol homeostasis. It binds to DNA at specific LXR response elements usually forming heterodimers with the retinoid X receptor (RXR).

Biological function summary

LXR alpha plays a critical role in lipid metabolism and cholesterol efflux. It is part of a larger LXR and CO complex which contributes to the regulation of genes that manage cholesterol absorption transport and excretion. For instance LXR activation increases the expression of ATP-binding cassette transporters such as ABCA1 and ABCG1 which are essential for transporting cholesterol out of cells and facilitating reverse cholesterol transport.

Pathways

The function of LXR alpha is significant in both the cholesterol catabolism pathway and the inflammatory response pathway. Within these pathways it upregulates genes involved in cholesterol catabolism providing a mechanism to prevent excess accumulation in cells and tissues. LXR alpha interacts closely with other nuclear receptors and proteins like PPARs (peroxisome proliferator-activated receptors) highlighting its role in lipid signaling networks.

LXR alpha is linked to atherosclerosis and Alzheimer’s disease. Its ability to regulate cholesterol transport and immune function demonstrates involvement in the pathogenesis of atherosclerosis by influencing macrophage-derived foam cell formation. Additionally improper cholesterol metabolism is associated with Alzheimer’s disease where LXR alpha's interaction with amyloid precursor protein (APP) may influence disease progression connecting cholesterol dysfunction with neurodegenerative processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed : 19481530, PubMed : 25661920, PubMed : 37478846). Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES (PubMed : 37478846). LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides (By similarity). Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8 (PubMed : 19481530). Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity). Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis (By similarity). Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators (By similarity).

See full target information NR1H3

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Publications (73)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:193 PubMed40268915

2025

High-fat diet promotes lipotoxicity in the podocytes of uninephrectomized mice: a targeted lipidomics and kidney podocyte-specific analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Se-Hyun Oh,You-Jin Kim,Subin Bae,Hee-Yeon Jung,So-Young Park,Jeong-Hoon Lim,Jang-Hee Cho,Chan-Duck Kim,Sun-Hee Park,Tae-Hwan Kwon,Yong-Jin Kim,Kwang-Hyeon Liu,Yong-Lim Kim

Molecular medicine (Cambridge, Mass.) 31:99 PubMed40087552

2025

LXRα agonists ameliorates acute rejection after liver transplantation via ABCA1/MAPK and PI3K/AKT/mTOR signaling axis in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Qin,Dingheng Hu,Qi Li,Shiyi Zhang,Zheng Qin,Liangxu Wang,Rui Liao,Zhongjun Wu,Yanyao Liu

International journal of chronic obstructive pulmonary disease 20:411-423 PubMed40008109

2025

Cholesterol Accumulation Enhances Cigarette Smoke-Induced Airway Epithelial Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Du Jing,Jin-Kang Yu,Hai-Pin Chen,Ling-Ling Dong,Wen Li,Zhou-Yang Li,Jie-Sen Zhou

Frontiers in pharmacology 15:1442279 PubMed39564113

2024

Chaihu Shugan powder restores fatty acid synthesis to alleviate insulin resistance in metabolic syndrome by regulating the LXRα/SREBP-1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sisi Lei,Weihang Peng,Lulu Wu,Liyuan Yu,Meida Wang,Qingmin Li,Yi Deng,Shuai Zhao,Peiying Huang,Bojun Chen

Histology and histopathology 40:585-596 PubMed39315497

2024

The Lian-Dou-Qing-Mai Formula activates the PPARγ-LXRα-ABCA1/ABCG1 pathway by regulating IL-10, leading to the promotion of cholesterol efflux and a reduction in atherosclerotic plaques.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqi Liao,You Li,Haoyan Zhao,Shu Lu

Frontiers in microbiology 15:1387401 PubMed38860223

2024

Sheng-Jiang powder ameliorates NAFLD via regulating intestinal microbiota in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Pengcheng Zhang,Juan Li,Yifan Miao,Xianlin Zhao,Lv Zhu,Jiaqi Yao,Meihua Wan,Wenfu Tang

Journal of translational medicine 22:352 PubMed38622667

2024

Quinic acid regulated TMA/TMAO-related lipid metabolism and vascular endothelial function through gut microbiota to inhibit atherosclerotic.

Applications

Unspecified application

Species

Unspecified reactive species

Qiao Jin,Chiyuan Zhang,Ran Chen,Luping Jiang,Hongli Li,Pengcui Wu,Liang Li

Brain, behavior, & immunity - health 38:100757 PubMed38590761

2024

Cholesterol-dependent LXR transcription factor activity represses pronociceptive effects of estrogen in sensory neurons and pain induced by myelin basic protein fragments.

Applications

Unspecified application

Species

Unspecified reactive species

Swathi K Hullugundi,Jennifer Dolkas,Andrei V Chernov,Tony L Yaksh,Kelly A Eddinger,Mila Angert,Glaucilene Ferreira Catroli,Alex Y Strongin,Patrick M Dougherty,Yan Li,Oswal Quehenberger,Aaron Armando,Veronica I Shubayev

Journal of pharmaceutical analysis 13:1281-1295 PubMed38174118

2024

Oridonin restores hepatic lipid homeostasis in an LXRα-ATGL/EPT1 axis-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Yulian Chen,Huanguo Jiang,Zhikun Zhan,Jindi Lu,Tanwei Gu,Ping Yu,Weimin Liang,Xi Zhang,Shilong Zhong,Lan Tang

Heliyon 9:e22785 PubMed38089978

2023

Protective effects of methyl protodioscin against lipid disorders and liver injury in hyperlipidemic gerbils.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojia Chen,Pengfei Zhang,Weilie Ma,Haiqiang Pan,Weitao Hong,Gengji Chen,Hang Ding,Wanze Tang,Guorong Lin,Zhizhen Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LXR alpha antibody [EPR6508(N)]

Western blot - Anti-LXR alpha antibody [EPR6508(N)] (AB176323)

Western blot - Anti-LXR alpha antibody [EPR6508(N)] (AB176323)

Western blot - Anti-LXR alpha antibody [EPR6508(N)] (AB176323)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (73)

Product promise