AB190727

Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal LXR alpha antibody. Carrier free. Suitable for WB and reacts with Human, Transfected cell line - Human samples. Cited in 1 publication.

View Alternative Names

LXRA, NR1H3, Oxysterols receptor LXR-alpha, Liver X receptor alpha, Nuclear receptor subfamily 1 group H member 3

3 Images

Lab

Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (AB190727)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM/TBST ab176323 does not cross activate with human LXR beta. ab213204 was used as loading control. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176323).

All lanes:

Western blot - Anti-LXR alpha antibody [EPR6508(N)] (<a href='/en-us/products/primary-antibodies/lxr-alpha-antibody-epr6508n-ab176323'>ab176323</a>) at 1/1000 dilution

Lane 1:

HEK 293 transfected with blank vectors, whole cell lysate at 20 µg

Lane 2:

HEK 293 transfected with His tagged human LXR alpha full length expression vectors, whole cell lysate at 20 µg

Lane 3:

HEK 293 transfected with His tagged human LXR beta full length expression vectors, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Exposure time: 3s

Lab

Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (AB190727)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM/TBST ab176323 detects a higher band in nuclear fraction than in non-nuclear fraction, which might be the correct band of interest. ab176842 and ab181602 were used as loading controls. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176323).

All lanes:

Western blot - Anti-LXR alpha antibody [EPR6508(N)] (<a href='/en-us/products/primary-antibodies/lxr-alpha-antibody-epr6508n-ab176323'>ab176323</a>) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) cells non-nuclear fraction at 20 µg

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) cells nuclear fraction at 20 µg

Lane 3:

SH-SY5Y (Human neuroblastoma epithelial cell) cells non-nuclear fraction at 20 µg

Lane 4:

SH-SY5Y (Human neuroblastoma epithelial cell) cells nuclear fraction at 20 µg

Lane 5:

IMR-32 (Human neuroblastoma neuroblast) cells non-nuclear fraction at 20 µg

Lane 6:

IMR-32 (Human neuroblastoma neuroblast) cells nuclear fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Exposure time: 7s

Lab

Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (AB190727)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM/TBST ab176323 detects a higher band in nuclear fraction than in non-nuclear fraction, which might be the correct band of interest. ab213204, ab176842 and ab181602 were used as loading controls. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176323).

All lanes:

Western blot - Anti-LXR alpha antibody [EPR6508(N)] (<a href='/en-us/products/primary-antibodies/lxr-alpha-antibody-epr6508n-ab176323'>ab176323</a>) at 1/1000 dilution

Lane 1:

HEK 293 transfected with blank vectors, whole cell lysate at 20 µg

Lane 2:

HEK 293 transfected with blank vectors, non-nuclear fraction at 20 µg

Lane 3:

HEK 293 transfected with blank vectors, nuclear fraction at 20 µg

Lane 4:

HEK 293 transfected with His tagged human LXR alpha full length expression vectors, non-nuclear fraction at 20 µg

Lane 5:

HEK 293 transfected with His tagged human LXR alpha full length expression vectors, nuclear fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa

false

Exposure time: 10s

Unconjugated

Anti-LXR alpha antibody [EPR6508(N)]
HRP

HRP Anti-LXR alpha antibody [EPR6508(N)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6508(N)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Can be blocked with LXR alpha peptide (<a href='/en-us/products/proteins-peptides/lxr-alpha-peptide-ab191835'>ab191835</a>).", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Sheep": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell line - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Can be blocked with LXR alpha peptide (<a href='/en-us/products/proteins-peptides/lxr-alpha-peptide-ab191835'>ab191835</a>).", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab190727 is the carrier-free version of ab176323.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LXR alpha also known as NR1H3 is a nuclear receptor protein involved in the regulation of lipid metabolism and inflammation. It has a molecular mass of approximately 55 kDa. Expressed mainly in the liver adipose tissue macrophages and intestines LXR alpha acts as a transcription factor controlling the expression of genes important for cholesterol homeostasis. It binds to DNA at specific LXR response elements usually forming heterodimers with the retinoid X receptor (RXR).

Biological function summary

LXR alpha plays a critical role in lipid metabolism and cholesterol efflux. It is part of a larger LXR and CO complex which contributes to the regulation of genes that manage cholesterol absorption transport and excretion. For instance LXR activation increases the expression of ATP-binding cassette transporters such as ABCA1 and ABCG1 which are essential for transporting cholesterol out of cells and facilitating reverse cholesterol transport.

Pathways

The function of LXR alpha is significant in both the cholesterol catabolism pathway and the inflammatory response pathway. Within these pathways it upregulates genes involved in cholesterol catabolism providing a mechanism to prevent excess accumulation in cells and tissues. LXR alpha interacts closely with other nuclear receptors and proteins like PPARs (peroxisome proliferator-activated receptors) highlighting its role in lipid signaling networks.

LXR alpha is linked to atherosclerosis and Alzheimer’s disease. Its ability to regulate cholesterol transport and immune function demonstrates involvement in the pathogenesis of atherosclerosis by influencing macrophage-derived foam cell formation. Additionally improper cholesterol metabolism is associated with Alzheimer’s disease where LXR alpha's interaction with amyloid precursor protein (APP) may influence disease progression connecting cholesterol dysfunction with neurodegenerative processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed : 19481530, PubMed : 25661920, PubMed : 37478846). Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES (PubMed : 37478846). LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides (By similarity). Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8 (PubMed : 19481530). Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity). Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis (By similarity). Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators (By similarity).

See full target information NR1H3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncogenesis 10:13 PubMed33568624

2021

TGF-β1-mediated transition of resident fibroblasts to cancer-associated fibroblasts promotes cancer metastasis in gastrointestinal stromal tumor.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunho Yoon,Chih-Min Tang,Sudeep Banerjee,Antonio L Delgado,Mayra Yebra,Jacob Davis,Jason K Sicklick

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free

Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (AB190727)

Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (AB190727)

Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (AB190727)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise