Rabbit Recombinant Monoclonal LXR alpha antibody. Carrier free. Suitable for WB and reacts with Human, Transfected cell line - Human samples.
Images
![Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (AB190727), expandable thumbnail](https://content.abcam.com/products/images/lxr-alpha-antibody-epr6508n-bsa-and-azide-free-ab190727--western-blot-img407533.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (AB190727)")
![Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (AB190727), expandable thumbnail](https://content.abcam.com/products/images/lxr-alpha-antibody-epr6508n-bsa-and-azide-free-ab190727--western-blot-img407532.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (AB190727)")
![Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (AB190727), expandable thumbnail](https://content.abcam.com/products/images/lxr-alpha-antibody-epr6508n-bsa-and-azide-free-ab190727--western-blot-img407531.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (AB190727)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Predicted | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Predicted | Not recommended | Not recommended | Not recommended |
| Sheep | Not recommended | Predicted | Not recommended | Not recommended | Not recommended |
| Transfected cell line - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Sheep, Transfected cell line - Human | Dilution info - | Notes - |
WB
TestedTested
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Sheep, Transfected cell line - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Sheep, Transfected cell line - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species Sheep | Dilution info - | Notes - |
Species Transfected cell line - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
1 product for Alternative Version
Target data
Function
Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed:19481530, PubMed:25661920, PubMed:37478846). Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES (PubMed:37478846). LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides (By similarity). Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8 (PubMed:19481530). Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity). Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis (By similarity). Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators (By similarity).
Alternative names
LXRA, NR1H3, Oxysterols receptor LXR-alpha, Liver X receptor alpha, Nuclear receptor subfamily 1 group H member 3
Recommended products
Rabbit Recombinant Monoclonal LXR alpha antibody. Carrier free. Suitable for WB and reacts with Human, Transfected cell line - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6508(N)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab190727 is the carrier-free version of Anti-LXR alpha antibody [EPR6508(N)] ab176323.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LXR alpha also known as NR1H3 is a nuclear receptor protein involved in the regulation of lipid metabolism and inflammation. It has a molecular mass of approximately 55 kDa. Expressed mainly in the liver adipose tissue macrophages and intestines LXR alpha acts as a transcription factor controlling the expression of genes important for cholesterol homeostasis. It binds to DNA at specific LXR response elements usually forming heterodimers with the retinoid X receptor (RXR).
- Biological function summary
LXR alpha plays a critical role in lipid metabolism and cholesterol efflux. It is part of a larger LXR and CO complex which contributes to the regulation of genes that manage cholesterol absorption transport and excretion. For instance LXR activation increases the expression of ATP-binding cassette transporters such as ABCA1 and ABCG1 which are essential for transporting cholesterol out of cells and facilitating reverse cholesterol transport.
- Pathways
The function of LXR alpha is significant in both the cholesterol catabolism pathway and the inflammatory response pathway. Within these pathways it upregulates genes involved in cholesterol catabolism providing a mechanism to prevent excess accumulation in cells and tissues. LXR alpha interacts closely with other nuclear receptors and proteins like PPARs (peroxisome proliferator-activated receptors) highlighting its role in lipid signaling networks.
- Associated diseases and disorders
LXR alpha is linked to atherosclerosis and Alzheimer’s disease. Its ability to regulate cholesterol transport and immune function demonstrates involvement in the pathogenesis of atherosclerosis by influencing macrophage-derived foam cell formation. Additionally improper cholesterol metabolism is associated with Alzheimer’s disease where LXR alpha's interaction with amyloid precursor protein (APP) may influence disease progression connecting cholesterol dysfunction with neurodegenerative processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (ab190727), expandable thumbnail](https://content.abcam.com/products/images/lxr-alpha-antibody-epr6508n-bsa-and-azide-free-ab190727--western-blot-img407533.jpg "Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (ab190727)")
Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (ab190727)
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
Anti-LXR alpha antibody [EPR6508(N)] ab176323 detects a higher band in nuclear fraction than in non-nuclear fraction, which might be the correct band of interest.
Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 were used as loading controls.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-LXR alpha antibody [EPR6508(N)] ab176323).All lanes: Western blot - Anti-LXR alpha antibody [EPR6508(N)] (Anti-LXR alpha antibody [EPR6508(N)] ab176323) at 1/1000 dilution
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) cells non-nuclear fraction at 20 µg
Lane 2: Jurkat (Human T cell leukemia T lymphocyte) cells nuclear fraction at 20 µg
Lane 3: SH-SY5Y (Human neuroblastoma epithelial cell) cells non-nuclear fraction at 20 µg
Lane 4: SH-SY5Y (Human neuroblastoma epithelial cell) cells nuclear fraction at 20 µg
Lane 5: IMR-32 (Human neuroblastoma neuroblast) cells non-nuclear fraction at 20 µg
Lane 6: IMR-32 (Human neuroblastoma neuroblast) cells nuclear fraction at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 7s
![Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (ab190727), expandable thumbnail](https://content.abcam.com/products/images/lxr-alpha-antibody-epr6508n-bsa-and-azide-free-ab190727--western-blot-img407532.jpg "Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (ab190727)")
Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (ab190727)
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
Anti-LXR alpha antibody [EPR6508(N)] ab176323 detects a higher band in nuclear fraction than in non-nuclear fraction, which might be the correct band of interest.
Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 were used as loading controls.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-LXR alpha antibody [EPR6508(N)] ab176323).All lanes: Western blot - Anti-LXR alpha antibody [EPR6508(N)] (Anti-LXR alpha antibody [EPR6508(N)] ab176323) at 1/1000 dilution
Lane 1: HEK 293 transfected with blank vectors, whole cell lysate at 20 µg
Lane 2: HEK 293 transfected with blank vectors, non-nuclear fraction at 20 µg
Lane 3: HEK 293 transfected with blank vectors, nuclear fraction at 20 µg
Lane 4: HEK 293 transfected with His tagged human LXR alpha full length expression vectors, non-nuclear fraction at 20 µg
Lane 5: HEK 293 transfected with His tagged human LXR alpha full length expression vectors, nuclear fraction at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 10s
![Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (ab190727), expandable thumbnail](https://content.abcam.com/products/images/lxr-alpha-antibody-epr6508n-bsa-and-azide-free-ab190727--western-blot-img407531.jpg "Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (ab190727)")
Western blot - Anti-LXR alpha antibody [EPR6508(N)] - BSA and Azide free (ab190727)
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
Anti-LXR alpha antibody [EPR6508(N)] ab176323 does not cross activate with human LXR beta.
Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204 was used as loading control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-LXR alpha antibody [EPR6508(N)] ab176323).All lanes: Western blot - Anti-LXR alpha antibody [EPR6508(N)] (Anti-LXR alpha antibody [EPR6508(N)] ab176323) at 1/1000 dilution
Lane 1: HEK 293 transfected with blank vectors, whole cell lysate at 20 µg
Lane 2: HEK 293 transfected with His tagged human LXR alpha full length expression vectors, whole cell lysate at 20 µg
Lane 3: HEK 293 transfected with His tagged human LXR beta full length expression vectors, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 3s
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