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Mouse Monoclonal LXR alpha antibody. Suitable for ELISA, IP, EMSA, ChIP, Flow Cyt, IHC-P, GSA, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 81 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NR1H3 aa 150 to C-terminus.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (AB41902), expandable thumbnail
  • Flow Cytometry - Anti-LXR alpha antibody [PPZ0412] (AB41902), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (AB41902), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (AB41902), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7
Preservative: 0.1% Sodium azide

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human NR1H3 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13133

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAIPEMSAChIPFlow CytWBIHC-PGSAICC/IF
Human
Expected
Expected
Expected
Expected
Tested
Not recommended
Tested
Expected
Expected
Mouse
Expected
Expected
Expected
Expected
Predicted
Not recommended
Predicted
Expected
Expected
Rat
Expected
Expected
Expected
Expected
Expected
Not recommended
Tested
Expected
Expected

Expected
Expected

Species
Human, Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Expected
Expected

Species
Mouse, Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Expected
Expected

Species
Mouse, Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Expected
Expected

Species
Mouse, Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat, Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Expected
Expected

Species
Mouse, Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed:19481530, PubMed:25661920, PubMed:37478846). Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES (PubMed:37478846). LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides (By similarity). Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8 (PubMed:19481530). Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity). Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis (By similarity). Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators (By similarity).

Alternative names

LXRA, NR1H3, Oxysterols receptor LXR-alpha, Liver X receptor alpha, Nuclear receptor subfamily 1 group H member 3

Recommended products

Mouse Monoclonal LXR alpha antibody. Suitable for ELISA, IP, EMSA, ChIP, Flow Cyt, IHC-P, GSA, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 81 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NR1H3 aa 150 to C-terminus.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human NR1H3 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13133
Clone number
PPZ0412
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 3rd April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LXR alpha also known as NR1H3 is a nuclear receptor protein involved in the regulation of lipid metabolism and inflammation. It has a molecular mass of approximately 55 kDa. Expressed mainly in the liver adipose tissue macrophages and intestines LXR alpha acts as a transcription factor controlling the expression of genes important for cholesterol homeostasis. It binds to DNA at specific LXR response elements usually forming heterodimers with the retinoid X receptor (RXR).

Biological function summary

LXR alpha plays a critical role in lipid metabolism and cholesterol efflux. It is part of a larger LXR and CO complex which contributes to the regulation of genes that manage cholesterol absorption transport and excretion. For instance LXR activation increases the expression of ATP-binding cassette transporters such as ABCA1 and ABCG1 which are essential for transporting cholesterol out of cells and facilitating reverse cholesterol transport.

Pathways

The function of LXR alpha is significant in both the cholesterol catabolism pathway and the inflammatory response pathway. Within these pathways it upregulates genes involved in cholesterol catabolism providing a mechanism to prevent excess accumulation in cells and tissues. LXR alpha interacts closely with other nuclear receptors and proteins like PPARs (peroxisome proliferator-activated receptors) highlighting its role in lipid signaling networks.

Associated diseases and disorders

LXR alpha is linked to atherosclerosis and Alzheimer’s disease. Its ability to regulate cholesterol transport and immune function demonstrates involvement in the pathogenesis of atherosclerosis by influencing macrophage-derived foam cell formation. Additionally improper cholesterol metabolism is associated with Alzheimer’s disease where LXR alpha's interaction with amyloid precursor protein (APP) may influence disease progression connecting cholesterol dysfunction with neurodegenerative processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (ab41902), expandable thumbnail
    This image is courtesy of a customer review submitted by Rudolf Jung, University of Regensburg.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (ab41902)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling LXR alpha with ab41902 at 1/100 dilution. Tissue was fixed with formaldehyde and permeabilized with Tween 20. Heat mediated antigen retrieval was performed using TRIS-EDTA Buffer. Tissue sections were incubated with Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902) for 30 minutes at 20°C in diluent from DAKO. An undiluted HRP conjugated horse anti-mouse secondary antibody was used.

    This image was generated using the ascites version of the product.

  • Flow Cytometry - Anti-LXR alpha antibody [PPZ0412] (ab41902), expandable thumbnail

    Flow Cytometry - Anti-LXR alpha antibody [PPZ0412] (ab41902)

    Overlay histogram showing HepG2 cells stained with ab41902 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab41902, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (ab41902), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (ab41902)

    ab41902 staining LXR alpha in rat spleen macrophage cell paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (ab41902), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (ab41902)

    ab41902 staining LXR alpha in Rat Hepatocyte and Kupffer cell paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.

    This image was generated using the ascites version of the product.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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