AB41902

Anti-LXR alpha antibody [PPZ0412]

Name: Anti-LXR alpha antibody [PPZ0412] (ab41902) | Abcam
Brand: Abcam Limited
SKU: AB41902
Price: 380 USD
Availability: InStock
Rating: 4 (12 reviews)

(12 Reviews)

(91 Publications)

Anti-LXR alpha antibody [PPZ0412] (ab41902) is a mouse monoclonal antibody detecting LXR alpha in Flow Cytometry, IP, IHC-P, ICC/IF, ELISA, EM, ChIP. Suitable for Human, Mouse, Rat.

- Over 80 publications
- Trusted since 2006

View Alternative Names

LXRA, NR1H3, Oxysterols receptor LXR-alpha, Liver X receptor alpha, Nuclear receptor subfamily 1 group H member 3

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (AB41902)

IHC-P

AbReview13621****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (AB41902)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling LXR alpha with ab41902 at 1/100 dilution. Tissue was fixed with formaldehyde and permeabilized with Tween 20. Heat mediated antigen retrieval was performed using TRIS-EDTA Buffer. Tissue sections were incubated with Anti-LXR alpha antibody [PPZ0412] - ChIP Grade (ab41902) for 30 minutes at 20°C in diluent from DAKO. An undiluted HRP conjugated horse anti-mouse secondary antibody was used.

This image was generated using the ascites version of the product.

This image is courtesy of an abreview submitted by Rudolf Jung, University of Regensburg.

Flow Cytometry - Anti-LXR alpha antibody [PPZ0412] (AB41902)

Flow Cyt

Unknown

Flow Cytometry - Anti-LXR alpha antibody [PPZ0412] (AB41902)

Overlay histogram showing HepG2 cells stained with ab41902 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab41902, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was generated using the ascites version of the product.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (AB41902)

ab41902 staining LXR alpha in Rat Hepatocyte and Kupffer cell paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.

This image was generated using the ascites version of the product.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (AB41902)

ab41902 staining LXR alpha in rat spleen macrophage cell paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

PPZ0412

Isotype

IgG2a

Carrier free

Reacts with

Rat, Human, Mouse

Applications

ChIP, IP, EMSA, GSA, Flow Cyt, IHC-P, ICC/IF, ELISA

applications

Immunogen

Recombinant Fragment Protein within Human NR1H3 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13133

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Reactivity data

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Product details

What is this antibody validated in?
Anti-LXR alpha antibody [PPZ0412] (ab41902) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, Electron microscopy (EM), ChIP in Human, Mouse, Rat samples.

Trusted by the scientific community
Anti-LXR alpha [PPZ0412] (ab41902) was first used in a scientific publication in 2006 and has been cited over 80 times in peer-reviewed journals.

Reviewed by scientists
Anti-LXR alpha [PPZ0412] (ab41902) has over 10 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7 Preservative: 0.1% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LXR alpha also known as NR1H3 is a nuclear receptor protein involved in the regulation of lipid metabolism and inflammation. It has a molecular mass of approximately 55 kDa. Expressed mainly in the liver adipose tissue macrophages and intestines LXR alpha acts as a transcription factor controlling the expression of genes important for cholesterol homeostasis. It binds to DNA at specific LXR response elements usually forming heterodimers with the retinoid X receptor (RXR).

Biological function summary

LXR alpha plays a critical role in lipid metabolism and cholesterol efflux. It is part of a larger LXR and CO complex which contributes to the regulation of genes that manage cholesterol absorption transport and excretion. For instance LXR activation increases the expression of ATP-binding cassette transporters such as ABCA1 and ABCG1 which are essential for transporting cholesterol out of cells and facilitating reverse cholesterol transport.

Pathways

The function of LXR alpha is significant in both the cholesterol catabolism pathway and the inflammatory response pathway. Within these pathways it upregulates genes involved in cholesterol catabolism providing a mechanism to prevent excess accumulation in cells and tissues. LXR alpha interacts closely with other nuclear receptors and proteins like PPARs (peroxisome proliferator-activated receptors) highlighting its role in lipid signaling networks.

LXR alpha is linked to atherosclerosis and Alzheimer’s disease. Its ability to regulate cholesterol transport and immune function demonstrates involvement in the pathogenesis of atherosclerosis by influencing macrophage-derived foam cell formation. Additionally improper cholesterol metabolism is associated with Alzheimer’s disease where LXR alpha's interaction with amyloid precursor protein (APP) may influence disease progression connecting cholesterol dysfunction with neurodegenerative processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed : 19481530, PubMed : 25661920, PubMed : 37478846). Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES (PubMed : 37478846). LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides (By similarity). Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8 (PubMed : 19481530). Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity). Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis (By similarity). Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators (By similarity).

See full target information NR1H3

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Publications (91)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in veterinary science 12:1654799 PubMed40874205

2025

Dietary fiber enhances milk yield in plateau dairy cows via activation of the rumen microbiota-mammary gland axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Li,Dongxu Wen,Ziwei Zhou,Quji Suolang,Wangmu Siwang,Lamu Kangji,Tuoxian Tang,Zhenjiang Liu,Yachun Wang

Nature metabolism 6:1922-1938 PubMed39322746

2024

Damaging mutations in liver X receptor-α are hepatotoxic and implicate cholesterol sensing in liver health.

Applications

Unspecified application

Species

Unspecified reactive species

Sam M Lockhart,Milan Muso,Ilona Zvetkova,Brian Y H Lam,Alessandra Ferrari,Erik Schoenmakers,Katie Duckett,Jack Leslie,Amy Collins,Beatriz Romartínez-Alonso,John A Tadross,Raina Jia,Eugene J Gardner,Katherine Kentistou,Yajie Zhao,Felix Day,Alexander Mörseburg,Kara Rainbow,Debra Rimmington,Matteo Mastantuoni,James Harrison,Meritxell Nus,Khalid Guma'a,Sam Sherratt-Mayhew,Xiao Jiang,Katherine R Smith,Dirk S Paul,Benjamin Jenkins,Albert Koulman,Maik Pietzner,Claudia Langenberg,Nicholas Wareham,Giles S Yeo,Krishna Chatterjee,John Schwabe,Fiona Oakley,Derek A Mann,Peter Tontonoz,Anthony P Coll,Ken Ong,John R B Perry,Stephen O'Rahilly

Oncogene 43:3277-3290 PubMed39300255

2024

Single-cell RNA-sequencing reveals a unique landscape of the tumor microenvironment in obesity-associated breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Guanghui Zhao,Xiaodong Zhang,Liying Meng,Ke Dong,Shipeng Shang,Tengfei Jiang,Ziqian Liu,Haidong Gao

Journal of pharmaceutical analysis 13:1281-1295 PubMed38174118

2024

Oridonin restores hepatic lipid homeostasis in an LXRα-ATGL/EPT1 axis-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Yulian Chen,Huanguo Jiang,Zhikun Zhan,Jindi Lu,Tanwei Gu,Ping Yu,Weimin Liang,Xi Zhang,Shilong Zhong,Lan Tang

Cell death & disease 14:372 PubMed37355685

2023

TRIB1 regulates liver regeneration by antagonizing the NRF2-mediated antioxidant response.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyue Sun,Shuai Wang,Xiulian Miao,Sheng Zeng,Yan Guo,Anqi Zhou,Ying Chen,Yifei Chen,Fangqiao Lv,Zhiwen Fan,Yutong Wang,Yong Xu,Zilong Li

Antioxidants (Basel, Switzerland) 12: PubMed36830035

2023

Di-(2-ethylhexyl) Phthalate Limits the Lipid-Lowering Effects of Simvastatin by Promoting Protein Degradation of Low-Density Lipoprotein Receptor: Role of PPARγ-PCSK9 and LXRα-IDOL Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Bei-Chia Guo,Ko-Lin Kuo,Jenq-Wen Huang,Chia-Hui Chen,Der-Cherng Tarng,Tzong-Shyuan Lee

Cell death & disease 14:85 PubMed36746922

2023

Restoration of lipid homeostasis between TG and PE by the LXRα-ATGL/EPT1 axis ameliorates hepatosteatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yulian Chen,Huanguo Jiang,Zhikun Zhan,Jindi Lu,Tanwei Gu,Ping Yu,Weimin Liang,Xi Zhang,Shuwen Liu,Huichang Bi,Shilong Zhong,Lan Tang

Frontiers in genetics 13:1067826 PubMed36699456

2023

Low expression of NR1H3 correlates with macrophage infiltration and indicates worse survival in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Jiawen Zhang,Weiwei Zhao,Qingxian Li,Wenwu Cheng

Oxidative medicine and cellular longevity 2022:2226168 PubMed36518993

2022

Qihuang Zhuyu Formula Attenuates Atherosclerosis via Targeting PPAR to Regulate Cholesterol Efflux and Endothelial Cell Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Mengxi Wang,Qian Xiang,Weixin Sun,Haowen Zhang,Ruijie Shi,Jun Guo,Huaqin Tong,Manlu Fan,Yuhan Ding,Haibo Shi,Peng Yu,Le Shen,Qiong Wang,Xiaohu Chen

International journal of molecular sciences 23: PubMed36293338

2022

The Citrus Flavonoid Nobiletin Downregulates Angiopoietin-like Protein 3 (ANGPTL3) Expression and Exhibits Lipid-Modulating Effects in Hepatic Cells and Adult Zebrafish Models.

Applications

Unspecified application

Species

Unspecified reactive species

Ching-Yen Lin,Pei-Yi Chen,Hao-Jen Hsu,Wan-Yun Gao,Ming-Jiuan Wu,Jui-Hung Yen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LXR alpha antibody [PPZ0412]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (AB41902)

Flow Cytometry - Anti-LXR alpha antibody [PPZ0412] (AB41902)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (AB41902)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LXR alpha antibody [PPZ0412] (AB41902)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (91)

Product promise