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AB308041

Anti-LXR beta/NER antibody [RP23040144]

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Rabbit Recombinant Multiclonal LXR beta/NER antibody. Suitable for WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human NR1H2.

View Alternative Names

LXRB, NER, UNR, NR1H2, Oxysterols receptor LXR-beta, Liver X receptor beta, Nuclear receptor NER, Nuclear receptor subfamily 1 group H member 2, Ubiquitously-expressed nuclear receptor

1 Images
Western blot - Anti-LXR beta/NER antibody [RP23040144] (AB308041)
  • WB

Supplier Data

Western blot - Anti-LXR beta/NER antibody [RP23040144] (AB308041)

Western blot analysis was performed on nuclear extracts (30 µg lysate) of Jurkat (Lane 1), PC-3 (Lane 2), A549 (Lane 3), HeLa (Lane 4), HEK-293 (Lane 5), U-87 MG (Lane 6) and tissue extract of mouse muscle (Lane 7). The blots were probed with ab308041, 1-2 µg/mL and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.4 µg/mL, 1 : 2500 dilution). A 51 kDa band corresponding to LXR beta/ NER was observed. Known quantity of protein samples were electrophoresed using 10% BisTris gel electrophoresis system and a pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).

All lanes:

Western blot - Anti-LXR beta/NER antibody [RP23040144] (ab308041) at 1 µg/mL

Lane 1:

Jurkat nuclear extract at 30 µg

Lane 2:

PC-3 at 30 µg

Lane 3:

A549 at 30 µg

Lane 4:

HeLa at 30 µg

Lane 5:

HEK-293 at 30 µg

Lane 6:

U-87 MG at 30 µg

Lane 7:

Mouse muscle tissue lysate at 30 µg

Secondary

All lanes:

HRP-conjugate Goat anti-Rabbit IgG (H+L) Secondary Antibody at 2500 µg/mL

Predicted band size: 51 kDa

Observed band size: 51 kDa

true

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040144

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Human NR1H2.

P55055

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Reactivity data

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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 99.91% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Liver X Receptor Beta (LXR beta) also known as NR1H2 is a nuclear receptor that functions as a transcription factor. It has a molecular mass of approximately 51 kDa. This receptor is primarily expressed in tissues like liver kidney adipose tissue and macrophages. LXR beta regulates genes involved in cholesterol homeostasis lipid metabolism and inflammation. Additionally LXR beta forms heterodimers with Retinoid X Receptors (RXRs) to regulate gene expression in response to ligands.
Biological function summary

The protein plays a central role in the maintenance of lipid homeostasis and modulation of inflammatory responses. LXR beta acts within a complex system that also involves other nuclear receptors and co-regulators to effectively control gene transcription. Through its activity LXR beta impacts cholesterol efflux fatty acid synthesis and inflammatory gene expression. It helps preserve cellular lipid balance a critical factor for normal cellular function and overall organism health.

Pathways

LXR beta plays a significant role in the cholesterol metabolism and lipid homeostasis pathways. It activates the transcription of genes like ABCA1 and ABCG1 important for cholesterol efflux in macrophages and other cell types. LXR beta interacts closely with proteins such as SREBP-1c (Sterol Regulatory Element Binding Protein 1c) and RXR which are integral to lipid metabolism pathways. By modulating these pathways LXR beta contributes to reverse cholesterol transport reducing cholesterol overload in cells.

LXR beta has connections to atherosclerosis and metabolic syndrome. Alterations in LXR beta function can lead to impaired cholesterol efflux and excessive lipid accumulation contributing to atherosclerosis. In metabolic syndrome irregularities in LXR beta's regulation of lipid metabolism may exacerbate the disorder's associated risks. Its interaction with proteins like GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) further highlights the target's potential role in inflammatory conditions linked to these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed : 25661920). Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8; DLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism (By similarity). Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis (By similarity). Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles (By similarity). Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators (By similarity). Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor : inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex (PubMed : 20159957).
See full target information NR1H2
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