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AB109211

Anti-Ly-6A/E (Sca-1) antibody [EPR3355]

4

(1 Review)

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(14 Publications)

Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211) is a rabbit monoclonal antibody detecting Ly-6A/E (Sca-1) in Western Blot, IHC-P. Suitable for Mouse.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

Ly6, Ly6a, Lymphocyte antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, Stem cell antigen 1, T-cell-activating protein, SCA-1, TAP

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (AB109211)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (AB109211)

ab109211, at 1/50 dilution staining Ly6A/E (Sca1) in paraffin-embedded Mouse kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (AB109211)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (AB109211)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Ly6A/E (Sca1) with Purified ab109211 at 1 : 150 dilution (1.37 μg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (AB109211)
  • IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (AB109211)

Immunohistochemical analysis of murine bone marrow tissue, following total body irradiation, staining Ly-6A/E (Sca1) with ab109211. Sections were blocked with blocking reagent before staining with primary antibody and a peroxidase conjugated anti-rabbit IgG. Staining was detected with AEC substrate.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Image from Basile LA et al., PLoS One. 2012;7(2):e30434. Epub 2012 Feb 24. Fig 5.; doi:10.1371/journal.pone.0030434; February 24, 2012, PLoS ONE 7(2): e30434.

Western blot - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (AB109211)
  • WB

Lab

Western blot - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (AB109211)

All lanes:

Western blot - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211) at 1/1000 dilution

All lanes:

Mouse bone marrow lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 18 kDa

false

Western blot - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (AB109211)
  • WB

Lab

Western blot - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (AB109211)

All lanes:

Western blot - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211) at 1/10000 dilution

All lanes:

Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 18 kDa

false

  • Carrier free

    Anti-Ly-6A/E (Sca-1) antibody [EPR3355] - BSA and Azide free

  • 660 APC

    APC Anti-Ly-6A/E (Sca-1) antibody [EPR3355]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Ly-6A/E (Sca-1) antibody [EPR3355]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Ly-6A/E (Sca-1) antibody [EPR3355]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Ly-6A/E (Sca-1) antibody [EPR3355]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Ly-6A/E (Sca-1) antibody [EPR3355]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Ly-6A/E (Sca-1) antibody [EPR3355]

  • 578 PE

    PE Anti-Ly-6A/E (Sca-1) antibody [EPR3355]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3355

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p>Antigen retrieval is recommended.</p><p>For unpurified use at 1/50 - 1/100.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (ab109211) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Mouse samples.

What is the molecular weight of Ly-6A/E (Sca-1)?
Anti-Ly-6A/E (Sca-1) [EPR3355] (ab109211) specifically detects a band for Ly-6A/E (Sca-1) (UniProt: P05533) at a molecular weight of 14kDa.

Trusted by the scientific community
Anti-Ly-6A/E (Sca-1) [EPR3355] (ab109211) was first used in a scientific publication in 2011 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR3355] also available for your convenience: ab109211, Carrier free - ab239951, APC - ab319239, PE - ab319387, Alexa Fluor® 488 - ab319521, Alexa Fluor® 647 - ab319634, Alexa Fluor® 594 - ab319738, Alexa Fluor® 555 - ab319880, Alexa Fluor® 750 - ab320933

Species reactivity
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

T-cell activation.
See full target information Ly6a
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 15:1138-1152 PubMed38356711

2024

Aspartate β-hydroxylase Regulates Expression of Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Madiha Kanwal,Jana Smahelova,Barbora Ciharova,Shweta Dilip Johari,Jaroslav Nunvar,Mark Olsen,Michal Smahel

Tobacco induced diseases 22: PubMed38274000

2024

PU.1 alleviates the inhibitory effects of cigarette smoke on endothelial progenitor cell function and lung-homing through Wnt/β-catenin and CXCL12/CXCR4 pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Xue He,Yanan Cui,Tiao Li,Lijuan Luo,Zihang Zeng,Yiming Ma,Yan Chen

Science advances 9:eadf0927 PubMed36989360

2023

Aberrant cell state plasticity mediated by developmental reprogramming precedes colorectal cancer initiation.

Applications

Unspecified application

Species

Unspecified reactive species

Pratyusha Bala,Jonathan P Rennhack,Daulet Aitymbayev,Clare Morris,Sydney M Moyer,Gina N Duronio,Paul Doan,Zhixin Li,Xiaoyan Liang,Jason L Hornick,Matthew B Yurgelun,William C Hahn,Nilay S Sethi

Stem cell research & therapy 13:279 PubMed35765096

2022

Endoplasmic reticulum stress modulates the fate of lung resident mesenchymal stem cell to myofibroblast via C/EBP homologous protein during pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Yang,Wei Sun,Xiaoyan Jing,Qian Zhang,Hui Huang,Zuojun Xu

Nature communications 12:1412 PubMed33658506

2021

Minimally invasive delivery of therapeutic agents by hydrogel injection into the pericardial cavity for cardiac repair.

Applications

Unspecified application

Species

Unspecified reactive species

Dashuai Zhu,Zhenhua Li,Ke Huang,Thomas G Caranasos,Joseph S Rossi,Ke Cheng

Theranostics 11:1177-1191 PubMed33391529

2021

Apolipoprotein E, low-density lipoprotein receptor, and immune cells control blood-brain barrier penetration by AAV-PHP.eB in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Bao-Shu Xie,Xin Wang,Yao-Hua Pan,Gan Jiang,Jun-Feng Feng,Yong Lin

Molecular therapy. Methods & clinical development 17:1071-1078 PubMed32478122

2020

A Single Intravenous Injection of AAV-PHP.B- Ameliorates the Phenotype of Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Pedro Silva-Pinheiro,Raffaele Cerutti,Marta Luna-Sanchez,Massimo Zeviani,Carlo Viscomi

Human gene therapy 31:90-102 PubMed31696742

2019

Differential Expression in Blood-Brain Barrier Is Responsible for Strain Specific Central Nervous System Transduction Profile of AAV-PHP.B.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Rita Batista,Oliver D King,Christopher P Reardon,Crystal Davis, Shankaracharya,Vivek Philip,Heather Gray-Edwards,Neil Aronin,Cathleen Lutz,John Landers,Miguel Sena-Esteves

Biology of reproduction 100:390-397 PubMed30304517

2018

Characterization of cell fusion in an experimental mouse model of endometriosis†.

Applications

Unspecified application

Species

Unspecified reactive species

A Tal,R Tal,S Shaikh,S Gidicsin,R Mamillapalli,H S Taylor

Xenobiotica; the fate of foreign compounds in biological systems 49:740-752 PubMed29912608

2018

Liver-specific knockout of histone methyltransferase G9a impairs liver maturation and dysregulates inflammatory, cytoprotective, and drug-processing genes.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Lu,Xiaohong Lei,Qinghao Zhang
View all publications
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Product promise

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