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Rabbit Recombinant Monoclonal Ly6c antibody. Carrier free. Suitable for IHC-P, WB, mIHC and reacts with Mouse, Transfected cell line - Mouse, Transfected cell line - Human, Transfected cell lysate - Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (AB314121), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (AB314121), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (AB314121), expandable thumbnail
  • Western blot - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (AB314121), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (AB314121), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBmIHCFlow CytICC/IFIP
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell line - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell line - Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Mouse
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Transfected cell line - Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Transfected cell line - Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Rat, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Human, Transfected cell line - Mouse, Transfected cell line - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Rat, Transfected cell line - Mouse, Transfected cell line - Human, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat, Transfected cell line - Mouse, Transfected cell line - Human, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat, Transfected cell line - Mouse, Transfected cell line - Human, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat, Transfected cell line - Mouse, Transfected cell line - Human, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Alternative names

Lymphocyte antigen 6C1, Ly-6C1, Ly6c1

Recommended products

Rabbit Recombinant Monoclonal Ly6c antibody. Carrier free. Suitable for IHC-P, WB, mIHC and reacts with Mouse, Transfected cell line - Mouse, Transfected cell line - Human, Transfected cell lysate - Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27220-23
Purification technique
Affinity purification Protein A
Specificity

This antibody recognizes both Ly6c1 and Ly6c2.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab314121 is the carrier-free version of Anti-Ly6c antibody [EPR27220-23] ab314120.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Ly6c also known as Ly-6C is a protein marker with a molecular weight of approximately 18 kDa. It is part of the Ly6 family of glycosylphosphatidylinositol (GPI)-anchored cell surface proteins. Ly6c is predominantly expressed in mice on the surface of certain immune cells including monocytes macrophages dendritic cells and subsets of T cells. The expression levels can vary depending on the cell type and the activation or developmental stage of the cell. Ly6c serves as a useful marker in immunological studies to distinguish between different subsets of monocytes commonly identified as Ly6c^high and Ly6c^low populations.

Biological function summary

Ly6c is involved in the differentiation and trafficking of immune cells. It is not known to be part of a larger protein complex. In the immune system the Ly6c marker is critical for distinguishing between classical and non-classical monocytes in mice. Classical monocytes expressing high levels of Ly6c are involved in rapid response to inflammatory stimuli while non-classical monocytes with lower Ly6c expression help in patrolling blood vessels. This distinction is important in understanding how the immune system responds to various infections and how the body maintains immune homeostasis.

Pathways

Ly6c plays a role in the monocyte recruitment and differentiation pathways. It is closely related to the chemokine signaling pathway influencing how immune cells migrate towards sites of inflammation or injury. The presence of the Ly6c2 protein an isoform of Ly6c helps modulate these responses by interacting with cytokines and chemokines that guide the trafficking of immune cells. The presence of Ly6c influences the activity of proteins like CCR2 which plays a significant role in chemotaxis in response to inflammation.

Associated diseases and disorders

Ly6c is associated with inflammatory and autoimmune conditions. For example high Ly6c expression has been linked to inflammatory bowel disease (IBD) and atherosclerosis. In IBD the ability of Ly6c^high monocytes to migrate to the gut and promote inflammation is well-documented. Similarly in atherosclerosis the recruitment of Ly6c^high monocytes to blood vessels contributes to plaque formation. In these contexts proteins like MCP-1 (also known as CCL2) interact with Ly6c-expressing cells facilitating their movement and function in disease processes. Understanding the role of Ly6c in these conditions might help develop targeted therapies to mitigate chronic inflammation and related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (ab314121), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (ab314121)

    This data was developed using Anti-Ly6c antibody [EPR27220-23] ab314120, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Panel A: HEK-293T ( tissue labeling Ly6c with Anti-Ly6c antibody [EPR27220-23] ab314120 at 1/100 (5.19 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on (A) HEK-293T transfected with mouse LY6C1 expression vector and (B) HEK-293T cells transfected with a mouse LY6C2 protein expression vector, no staining on (C) HEK-293T cells transfected with a mouse Ly6i protein expression vector, (D) HEK-293T cells transfected with a mouse Ly6g protein expression vector, (E) HEK-293T cells transfected with a mouse Ly6a protein expression vector and (F) HEK-293T transfected with empty vector.The section was incubated with Anti-Ly6c antibody [EPR27220-23] ab314120 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control:Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (ab314121), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (ab314121)

    This data was developed using Anti-Ly6c antibody [EPR27220-23] ab314120, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Ly6c with Anti-Ly6c antibody [EPR27220-23] ab314120 at 1/100 followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse cerebrum vascular endothelium. The section was incubated with Anti-Ly6c antibody [EPR27220-23] ab314120 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control:Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (ab314121), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (ab314121)

    This data was developed using Anti-Ly6c antibody [EPR27220-23] ab314120, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Ly6c with Anti-Ly6c antibody [EPR27220-23] ab314120 at 1/100 (5.19 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse colon vascular endothelium. The section was incubated with Anti-Ly6c antibody [EPR27220-23] ab314120 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (ab314121), expandable thumbnail

    Western blot - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (ab314121)

    This data was developed using Anti-Ly6c antibody [EPR27220-23] ab314120, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This antibody recognizes both Ly6c1 and Ly6c2.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution
    Exposure time: 15 seconds

    All lanes: Western blot - Anti-Ly6c antibody [EPR27220-23] (Anti-Ly6c antibody [EPR27220-23] ab314120) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with a mouse Ly6c1 expression vector containing a HA-tag whole cell lysate at 10 µg

    Lane 2: 293T cells transfected with a mouse Ly6c2 expression vector containing a His-tag whole cell lysate at 10 µg

    Lane 3: 293T cells transfected with a mouse Ly6i expression vector containing a His-tag whole cell lysate at 20 µg

    Lane 4: 293T cells transfected with a mouse Ly6g expression vector containing a His-tag whole cell lysate at 20 µg

    Lane 5: 293T cells transfected with a mouse Ly6a expression vector containing a His-tag whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 14 kDa

    Exposure time: 15s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (ab314121), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (ab314121)

    This data was developed using Anti-Ly6c antibody [EPR27220-23] ab314120, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Ly6c with Anti-Ly6c antibody [EPR27220-23] ab314120 at 1/100 (5.19 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse spleen. The section was incubated with Anti-Ly6c antibody [EPR27220-23] ab314120 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control:Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (ab314121), expandable thumbnail

    Western blot - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (ab314121)

    This data was developed using Anti-Ly6c antibody [EPR27220-23] ab314120, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: testis, liver

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
    Exposure time: 92 seconds

    All lanes: Western blot - Anti-Ly6c antibody [EPR27220-23] (Anti-Ly6c antibody [EPR27220-23] ab314120) at 1/1000 dilution

    Lane 1: Mouse lung lysate at 20 µg

    Lane 2: Mouse spleen lysate at 20 µg

    Lane 3: Mouse testis lysate at 20 µg

    Lane 4: Mouse liver lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 14 kDa

    Exposure time: 92s

  • Multiplex immunohistochemistry - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (ab314121), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (ab314121)

    This data was produced using Anti-Ly6c antibody [EPR27220-23] ab314120, the same clone but in a different formulation.

    Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue staining Ly6c with Anti-Ly6c antibody [EPR27220-23] ab314120 at a 1/100 dilution ( 5.19 μg/ml), Anti-CD19 antibody [EPR23174-145] ab245235 anti-CD19 used at 1/1000 dilution (0.999 μg/ml), Anti-CD11b antibody [EPR1344] ab133357 anti-CD11b used at 1/20000 dilution (0.05 μg/ml).

    Panel A: merged staining of anti-Ly6c (green; Opal™690), anti-CD19 (gray; Opal™520) and anti-CD11b (red; Opal™570) on mouse spleen.
    Panel B: anti-Ly6c stained on monocytes/macrophages.
    Panel C: anti-CD19 stained on B cells.
    Panel D: anti-CD11b stained on monocytes/macrophages.

    The section was incubated in three rounds of staining: in the order of Anti-Ly6c antibody [EPR27220-23] ab314120, Anti-CD19 antibody [EPR23174-145] ab245235, and Anti-CD11b antibody [EPR1344] ab133357 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Western blot - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (ab314121), expandable thumbnail

    Western blot - Anti-Ly6c antibody [EPR27220-23] - BSA and Azide free (ab314121)

    This data was developed using Anti-Ly6c antibody [EPR27220-23] ab314120, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 92 seconds

    All lanes: Western blot - Anti-Ly6c antibody [EPR27220-23] (Anti-Ly6c antibody [EPR27220-23] ab314120) at 1/1000 dilution

    Lane 1: Mouse bone marrow lysate at 20 µg

    Lane 2: Mouse kidney lysate at 20 µg

    Lane 3: Mouse heart lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 14 kDa

    Exposure time: 92s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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