AB24973

Anti-Ly6c antibody [HK1.4]

Name: Anti-Ly6c antibody [HK1.4] (ab24973) | Abcam
Brand: Abcam Limited
SKU: AB24973
Price: 520 USD
Availability: InStock
Rating: 3 (2 reviews)

(2 Reviews)

(12 Publications)

Rat Monoclonal Ly6c antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 12 publications.

View Alternative Names

Lymphocyte antigen 6C1, Ly-6C1, Ly6c1

1 Images

Flow Cytometry - Anti-Ly6c antibody [HK1.4] (AB24973)

Flow Cyt

Unknown

Flow Cytometry - Anti-Ly6c antibody [HK1.4] (AB24973)

Bone marrow cells from C57BL/6 mice were labeled with ab24973 and then stained with goat anti-rat IgG-FITC. Large cells were gated and analyzed by flow cytometry.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

HK1.4

Isotype

IgG2c

Light chain type

kappa

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/unavailable/rat-igg2c-kappa-monoclonal-rtk4174-isotype-control-low-endotoxin-azide-free-ab18703'>ab18703</a> - Rat monoclonal IgG2c, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 8.2

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ly6c also known as Ly-6C is a protein marker with a molecular weight of approximately 18 kDa. It is part of the Ly6 family of glycosylphosphatidylinositol (GPI)-anchored cell surface proteins. Ly6c is predominantly expressed in mice on the surface of certain immune cells including monocytes macrophages dendritic cells and subsets of T cells. The expression levels can vary depending on the cell type and the activation or developmental stage of the cell. Ly6c serves as a useful marker in immunological studies to distinguish between different subsets of monocytes commonly identified as Ly6c^high and Ly6c^low populations.

Biological function summary

Ly6c is involved in the differentiation and trafficking of immune cells. It is not known to be part of a larger protein complex. In the immune system the Ly6c marker is critical for distinguishing between classical and non-classical monocytes in mice. Classical monocytes expressing high levels of Ly6c are involved in rapid response to inflammatory stimuli while non-classical monocytes with lower Ly6c expression help in patrolling blood vessels. This distinction is important in understanding how the immune system responds to various infections and how the body maintains immune homeostasis.

Pathways

Ly6c plays a role in the monocyte recruitment and differentiation pathways. It is closely related to the chemokine signaling pathway influencing how immune cells migrate towards sites of inflammation or injury. The presence of the Ly6c2 protein an isoform of Ly6c helps modulate these responses by interacting with cytokines and chemokines that guide the trafficking of immune cells. The presence of Ly6c influences the activity of proteins like CCR2 which plays a significant role in chemotaxis in response to inflammation.

Ly6c is associated with inflammatory and autoimmune conditions. For example high Ly6c expression has been linked to inflammatory bowel disease (IBD) and atherosclerosis. In IBD the ability of Ly6c^high monocytes to migrate to the gut and promote inflammation is well-documented. Similarly in atherosclerosis the recruitment of Ly6c^high monocytes to blood vessels contributes to plaque formation. In these contexts proteins like MCP-1 (also known as CCL2) interact with Ly6c-expressing cells facilitating their movement and function in disease processes. Understanding the role of Ly6c in these conditions might help develop targeted therapies to mitigate chronic inflammation and related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ly6c1

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Aging and disease 15:2632-2649 PubMed38270116

2024

NeuroD1 Regulated Endothelial Gene Expression to Modulate Transduction of AAV-PHP.eB and Recovery Progress after Ischemic Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaosong He,Xin Wang,Hui Wang,Tao Wang,Fuhan Yang,Yuchen Chen,Zifei Pei,Yuting Bai,Wen Li,Zheng Wu,Gong Chen

Journal of immunology (Baltimore, Md. : 1950) 210:180-190 PubMed36458991

2022

JMJD3 Is Required for Acute Pancreatitis and Pancreatitis-Associated Lung Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Li Chen,Xiangxian Zhang,Yu Liu,Li Liu,Xiao Liang,Shengqun Yang,Qing Xia,Tao Jin,Yun Ma,Yonghua Chen,Xia Yuan,Yan Tie,Yangzhuo Gu,Chunju Fang,Siyuan Chen,Fei Mo,Ting Yu,Yuzhu Hu,Zhiyong Qian,Yong Peng,Jia Geng,Zongguang Zhou,Min Wu,Jiansheng Ding,Daoke Yang,Xiawei Wei

Bioengineered 13:9687-9707 PubMed35420978

2022

Protective effect of growth differentiation factor 15 in sepsis by regulating macrophage polarization and its mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Xinliang Zhang,Shimin Dong

Small methods 6:e2100752 PubMed35023642

2022

Single-Cell RNA Sequencing Reveals the Temporal Diversity and Dynamics of Cardiac Immunity after Myocardial Infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiyu Jin,Shan Gao,Penghui Yang,Rongfang Guo,Dan Li,Yunsha Zhang,Xiaoyan Lu,Guanwei Fan,Xiaohui Fan

Frontiers in immunology 12:663548 PubMed33995391

2021

Alcohol Consumption Accumulation of Monocyte Derived Macrophages in Female Mice Liver Is Interferon Alpha Receptor Dependent.

Applications

Unspecified application

Species

Unspecified reactive species

Khaled Alharshawi,Holger Fey,Alyx Vogle,Tori Klenk,Miran Kim,Costica Aloman

The Journal of experimental medicine 216:556-570 PubMed30787033

2019

A major role for ferroptosis in -induced cell death and tissue necrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Eduardo P Amaral,Diego L Costa,Sivaranjani Namasivayam,Nicolas Riteau,Olena Kamenyeva,Lara Mittereder,Katrin D Mayer-Barber,Bruno B Andrade,Alan Sher

Leukemia 32:1435-1444 PubMed29556020

2018

Tracking preleukemic cells in vivo to reveal the sequence of molecular events in radiation leukemogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Tom Verbiest,Rosemary Finnon,Natalie Brown,Lourdes Cruz-Garcia,Paul Finnon,Grainne O'Brien,Eleanor Ross,Simon Bouffler,Cheryl L Scudamore,Christophe Badie

Cell death & disease 8:e3170 PubMed29144508

2017

Ly6G+ neutrophil-derived miR-223 inhibits the NLRP3 inflammasome in mitochondrial DAMP-induced acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zunyong Feng,Shimei Qi,Yue Zhang,Zhilin Qi,Liang Yan,Jing Zhou,Fang He,Qianqian Li,Yanyan Yang,Qun Chen,Shi Xiao,Qiang Li,Yang Chen,Yao Zhang

Experimental eye research 151:160-70 PubMed27544307

2016

Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models.

Applications

IHC-P

Species

Unspecified reactive species

Sidath E Liyanage,Peter J Gardner,Joana Ribeiro,Enrico Cristante,Robert D Sampson,Ulrich F O Luhmann,Robin R Ali,James W Bainbridge

The Journal of biological chemistry 289:17020-9 PubMed24798340

2014

Nuclear factor E2-related factor-2 (Nrf2) is required for NLRP3 and AIM2 inflammasome activation.

Applications

Flow Cyt

Species

Unspecified reactive species

Changcheng Zhao,Devyn D Gillette,Xinghui Li,Zhibin Zhang,Haitao Wen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Ly6c antibody [HK1.4]

Flow Cytometry - Anti-Ly6c antibody [HK1.4] (AB24973)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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