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Rabbit Recombinant Multiclonal Ly6c antibody. Suitable for IHC-P, WB, mIHC, ICC/IF, Flow Cyt, IP and reacts with Mouse samples.

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Images

Western blot - Anti-LY6C antibody [RM1151] (AB317272), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-LY6C antibody [RM1151] (AB317272), expandable thumbnail
  • Flow Cytometry - Anti-LY6C antibody [RM1151] (AB317272), expandable thumbnail
  • Flow Cytometry - Anti-LY6C antibody [RM1151] (AB317272), expandable thumbnail
  • Flow Cytometry - Anti-LY6C antibody [RM1151] (AB317272), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBmIHCICC/IFFlow CytIP
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Tested
Tested
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Target data

Function

Alternative names

Lymphocyte antigen 6C1, Ly-6C1, Ly6c1

Recommended products

Rabbit Recombinant Multiclonal Ly6c antibody. Suitable for IHC-P, WB, mIHC, ICC/IF, Flow Cyt, IP and reacts with Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

RM1151

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant multiclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Ly6c also known as Ly-6C is a protein marker with a molecular weight of approximately 18 kDa. It is part of the Ly6 family of glycosylphosphatidylinositol (GPI)-anchored cell surface proteins. Ly6c is predominantly expressed in mice on the surface of certain immune cells including monocytes macrophages dendritic cells and subsets of T cells. The expression levels can vary depending on the cell type and the activation or developmental stage of the cell. Ly6c serves as a useful marker in immunological studies to distinguish between different subsets of monocytes commonly identified as Ly6c^high and Ly6c^low populations.

Biological function summary

Ly6c is involved in the differentiation and trafficking of immune cells. It is not known to be part of a larger protein complex. In the immune system the Ly6c marker is critical for distinguishing between classical and non-classical monocytes in mice. Classical monocytes expressing high levels of Ly6c are involved in rapid response to inflammatory stimuli while non-classical monocytes with lower Ly6c expression help in patrolling blood vessels. This distinction is important in understanding how the immune system responds to various infections and how the body maintains immune homeostasis.

Pathways

Ly6c plays a role in the monocyte recruitment and differentiation pathways. It is closely related to the chemokine signaling pathway influencing how immune cells migrate towards sites of inflammation or injury. The presence of the Ly6c2 protein an isoform of Ly6c helps modulate these responses by interacting with cytokines and chemokines that guide the trafficking of immune cells. The presence of Ly6c influences the activity of proteins like CCR2 which plays a significant role in chemotaxis in response to inflammation.

Associated diseases and disorders

Ly6c is associated with inflammatory and autoimmune conditions. For example high Ly6c expression has been linked to inflammatory bowel disease (IBD) and atherosclerosis. In IBD the ability of Ly6c^high monocytes to migrate to the gut and promote inflammation is well-documented. Similarly in atherosclerosis the recruitment of Ly6c^high monocytes to blood vessels contributes to plaque formation. In these contexts proteins like MCP-1 (also known as CCL2) interact with Ly6c-expressing cells facilitating their movement and function in disease processes. Understanding the role of Ly6c in these conditions might help develop targeted therapies to mitigate chronic inflammation and related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

  • Western blot - Anti-LY6C antibody [RM1151] (ab317272), expandable thumbnail

    Western blot - Anti-LY6C antibody [RM1151] (ab317272)

    Negative control: Neuro-2a.

    Low expression: testis, liver.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-LY6C antibody [RM1151] (ab317272) at 1/1000 dilution

    Lane 1: Mouse lung tissue lysate at 20 µg

    Lane 2: Mouse spleen tissue lysate at 20 µg

    Lane 3: Mouse testis tissue lysate at 20 µg

    Lane 4: Mouse liver tissue lysate at 20 µg

    Lane 5: Mouse kidney tissue lysate at 20 µg

    Lane 6: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 7: C2C12 (mouse myoblast) whole cell lysate at 20 µg

    Lane 8: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 14 kDa, 36 kDa

    Exposure time: 180s

  • Multiplex immunohistochemistry - Anti-LY6C antibody [RM1151] (ab317272), expandable thumbnail

    Multiplex immunohistochemistry - Anti-LY6C antibody [RM1151] (ab317272)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spleen tissue staining LY6C with ab317272 at a 1:100 (0.978 ug/ml) dilution, Anti-CD11b antibody [EPR1344] ab133357 anti-CD11b used at 1:20000 (0.05 ug/ml) dilution and Anti-CD19 antibody [EPR23174-145] ab245235 anti-CD19 used at a 1:1000 (0.999 ug/ml) dilution.

    Panel A: merged staining of anti-Ly6c (green; Opal™690), anti-CD11b (magenta; Opal™570) and anti-CD19 (yellow; Opal™520) on mouse spleen.

    Panel B: anti-Ly6c stained on monocytes/macrophages.

    Panel C: anti-CD11b stained on monocytes/macrophages.

    Panel D: anti-CD19 stained on B cells.

    Co-staining of Ly6c and CD11b can be observed.

    The section was incubated in three rounds of staining: in the order of ab317272, Anti-CD11b antibody [EPR1344] ab133357, and Anti-CD19 antibody [EPR23174-145] ab245235 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Flow Cytometry - Anti-LY6C antibody [RM1151] (ab317272), expandable thumbnail

    Flow Cytometry - Anti-LY6C antibody [RM1151] (ab317272)

    Flow cytometric analysis of Mouse spleen cell cells labelling LY6C with ab317272 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Mouse spleen cell are co-stained with CD8a conjugated Alexa Fluor®647.

    Gated on viable cell.

  • Flow Cytometry - Anti-LY6C antibody [RM1151] (ab317272), expandable thumbnail

    Flow Cytometry - Anti-LY6C antibody [RM1151] (ab317272)

    Flow cytometric analysis of Mouse bone marrow cells labelling LY6C with ab317272 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Mouse bone marrow are co-stained with B220 conjugated Alexa Fluor®647.

    Gated on viable cell.

  • Flow Cytometry - Anti-LY6C antibody [RM1151] (ab317272), expandable thumbnail

    Flow Cytometry - Anti-LY6C antibody [RM1151] (ab317272)

    Flow cytometric analysis of Mouse bone marrow cells labelling LY6C with ab317272 at 1/500 dilution (0.1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Mouse bone marrow are co-stained with CD11b conjugated BV510 (512/25BP).

    Gated on viable cell.

  • Immunocytochemistry/ Immunofluorescence - Anti-LY6C antibody [RM1151] (ab317272), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-LY6C antibody [RM1151] (ab317272)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocyte cells labelling LY6C with ab317272 at 1/500 (0.978 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing cytoplasmic staining in subsets of mouse splenocytes (shown in green), which is partially co-located with CD11b. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Anti-CD11b antibody [M1/70] ab8878 Anti-CD11b Rat monoclonal antibody was used to counterstain at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-LY6C antibody [RM1151] (ab317272), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-LY6C antibody [RM1151] (ab317272)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse bone marrow cells labelling LY6C with ab317272 at 1/500 (0.978 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing cytoplasmic staining in subsets of mouse bone marrow (shown in green), which is partially co-located with CD11b. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Anti-CD11b antibody [M1/70] ab8878 Anti-CD11b Rat monoclonal antibody was used to counterstain at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] (ab317272), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] (ab317272)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling LY6C with ab317272 at 1/500 (0.978 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: no staining on mouse liver. The section was incubated with ab317272 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] (ab317272), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] (ab317272)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling LY6C with ab317272 at 1/500 (0.978 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on endothelial cells in mouse cerebrum. The section was incubated with ab317272 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] (ab317272), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] (ab317272)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling LY6C with ab317272 at 1/500 (0.978 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on endothelial cells and immune cells in mouse colon. The section was incubated with ab317272 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] (ab317272), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6C antibody [RM1151] (ab317272)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling LY6C with ab317272 at 1/500 (0.978 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on endothelial cells and immune cells in mouse spleen. The section was incubated with ab317272 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunoprecipitation - Anti-LY6C antibody [RM1151] (ab317272), expandable thumbnail

    Immunoprecipitation - Anti-LY6C antibody [RM1151] (ab317272)

    LY6C was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab317272 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317272 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse spleen tissue lysate

    Lane 2: ab317272 IP in Mouse spleen tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab317272 in mouse spleen tissue lysate.

    All lanes: Immunoprecipitation - Anti-LY6C antibody [RM1151] (ab317272) at 1/30 dilution

    All lanes: Mouse spleen tissue lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 128s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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