AB300411

Anti-LY6E/SCA-2 antibody [EPR26038-105] (BSA and Azide free)

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal LY6E/SCA-2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

9804, RIGE, SCA2, TSA1, LY6E, Lymphocyte antigen 6E, Ly-6E, Retinoic acid-induced gene E protein, Stem cell antigen 2, Thymic shared antigen 1, RIG-E, SCA-2, TSA-1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (BSA and Azide free) (AB300411)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (BSA and Azide free) (AB300411)

This data was developed using ab300399, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human ovarian carcin tissue labeling LY6E/SCA-2 with ab300399 at 1/500 (0.956 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Cytoplasmic staining on human ovarian carcinoma (PMID : 25862760).The section was incubated with ab300399 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (BSA and Azide free) (AB300411)

This data was developed using ab300399, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human breast carcino tissue labeling LY6E/SCA-2 with ab300399 at 1/500 (0.956 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Cytoplasmic staining on human breast carcinoma (PMID : 25862760).The section was incubated with ab300399 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (BSA and Azide free) (AB300411)

This data was developed using ab300399, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human gastric adenoc tissue labeling LY6E/SCA-2 with ab300399 at 1/500 (0.956 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Cytoplasmic staining on human gastric adenocarcinoma (PMID : 25862760).The section was incubated with ab300399 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (BSA and Azide free) (AB300411)

This data was developed using ab300399, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling LY6E/SCA-2 with ab300399 at 1/500 (0.956 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Negative control : almost no staining on human pancreas (PMID : 25862760).The section was incubated with ab300399 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Supplier Data

Western blot - Anti-LY6E/SCA-2 antibody [EPR26038-105] (BSA and Azide free) (AB300411)

This data was developed using ab300399, the same antibody clone in a different buffer formulation. Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-LY6E/SCA-2 antibody [EPR26038-105] (<a href='/en-us/products/primary-antibodies/ly6e-sca-2-antibody-epr26038-105-ab300399'>ab300399</a>) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate

Lane 2:

Human pancreas tissue lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 13 kDa

false

Exposure time: 48s

Supplier Data

Western blot - Anti-LY6E/SCA-2 antibody [EPR26038-105] (BSA and Azide free) (AB300411)

This data was developed using ab300399, the same antibody clone in a different buffer formulation. Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-LY6E/SCA-2 antibody [EPR26038-105] (<a href='/en-us/products/primary-antibodies/ly6e-sca-2-antibody-epr26038-105-ab300399'>ab300399</a>) at 1/1000 dilution

Lane 1:

Human ovary tissue lysate 20 µg

Lane 2:

Human thymus tissue lysate 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 13 kDa

false

Exposure time: 8s

Unconjugated

Anti-LY6E/SCA-2 antibody [EPR26038-105]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26038-105

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LY6E also known as SCA-2 is a membrane protein known for its role in immune response. With a mass of approximately 15 kDa LY6E is expressed on the cell surface in various tissues including the immune system and some epithelial cells. The protein belongs to the LY6 family which is characterized by the presence of a Ly6/uPAR domain playing a part in cellular signaling and host defense mechanisms.

Biological function summary

LY6E has a significant impact on immune modulation and viral infection processes. It interacts with other immune-related proteins and contributes to the regulation of T-cell proliferation and differentiation. While LY6E is not a part of a multi-protein complex itself its activity can modify the function of immune cells and potentially alter immune responses. This makes it an important player in the context of both adaptive and innate immunity.

Pathways

LY6E participates in key immune signaling pathways particularly those related to interferon signaling and antiviral defense. It influences these pathways by interacting with components such as interferon-gamma and other cytokines which regulate immune cell behavior and response. LY6E affects the STAT pathway by indirectly modulating the activity of STAT1 a critical protein in immune response highlighting its importance in orchestrating immune dynamics.

LY6E has been implicated in several viral infections and cancers. Its expression alters during viral infections such as influenza and HIV where it may play a role in enhancing or suppressing viral replication. LY6E's interaction with other proteins like STAT1 and various viral envelope proteins could contribute to immune escape or disease progression. Moreover aberrant regulation of LY6E levels has connections with cancer progression affecting tumor immunity and potentially making it a target for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GPI-anchored cell surface protein that regulates T-lymphocytes proliferation, differentiation, and activation. Regulates the T-cell receptor (TCR) signaling by interacting with component CD3Z/CD247 at the plasma membrane, leading to CD3Z/CD247 phosphorylation modulation (By similarity). Restricts the entry of human coronaviruses, including SARS-CoV, MERS-CoV and SARS-CoV-2, by interfering with spike protein-mediated membrane fusion (PubMed : 32641482). Also plays an essential role in placenta formation by acting as the main receptor for syncytin-A (SynA). Therefore, participates in the normal fusion of syncytiotrophoblast layer I (SynT-I) and in the proper morphogenesis of both fetal and maternal vasculatures within the placenta. May also act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity (By similarity).. (Microbial infection) Promotes entry, likely through an enhanced virus-cell fusion process, of various viruses including HIV-1, West Nile virus, dengue virus and Zika virus (PubMed : 28130445). In contrast, the paramyxovirus PIV5, which enters at the plasma membrane, does not require LY6E (PubMed : 28130445, PubMed : 29610346). Mechanistically, adopts a microtubule-like organization upon viral infection and enhances viral uncoating after endosomal escape (PubMed : 28130445, PubMed : 30190477).

See full target information LY6E

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LY6E/SCA-2 antibody [EPR26038-105] (BSA and Azide free)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (BSA and Azide free) (AB300411)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (BSA and Azide free) (AB300411)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (BSA and Azide free) (AB300411)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY6E/SCA-2 antibody [EPR26038-105] (BSA and Azide free) (AB300411)

Western blot - Anti-LY6E/SCA-2 antibody [EPR26038-105] (BSA and Azide free) (AB300411)

Western blot - Anti-LY6E/SCA-2 antibody [EPR26038-105] (BSA and Azide free) (AB300411)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise