Anti-Ly6g [1A8] antibody (ab210204) is a rat monoclonal antibody that is provided in an azide free formulation. It is used to detect Ly6g in Flow Cytometry, IP, IHC-P, IHC-Fr, FuncS. Suitable for Mouse samples. Available in 5mg, 10mg, 25mg and 50mg sizes for in-vivo use upon request.
- Suitable for in vitro and in vivo studies, including neutralization, blocking, or activation/proliferation
Images
![Flow Cytometry - Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free (AB210204), expandable thumbnail](https://content.abcam.com/products/images/ly6g-antibody-1a8-low-endotoxin-azide-free-ab210204--flow-cytometry-img445206.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free (AB210204)")
Publications
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- JCI insight 9:2024Efficient pulmonary lymphatic drainage is necessary for inflammation resolution in ARDS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPu-Hong Zhang,Wen-Wu Zhang,Shun-Shun Wang,Cheng-Hua Wu,Yang-Dong Ding,Xin-Yi Wu,Fang Gao Smith,Yu Hao,Sheng-Wei JinPubMed 37971881
- Frontiers in pharmacology 13:8695122022Circadian Dependence of the Acute Immune Response to Myocardial Infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAoife B Kilgallen,Frederieke van den Akker,Dries A M Feyen,Sandra Crnko,Christian J B Snijders Blok,Hendrik Gremmels,Bastiaan C du Pré,Robin Reijers,Pieter A Doevendans,Saskia C A de Jager,Joost P G Sluijter,Vasco Sampaio-Pinto,Linda W van LaakePubMed 35694249
- Theranostics 12:3024-30442022Critical role of FPR1 in splenocyte migration into brain to worsen inflammation and ischemic brain injury in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Li,Mahendra D Chordia,Yi Zhang,Hui Zong,Dongfeng Pan,Zhiyi ZuoPubMed 35547761
- Scientific reports 11:150732021Estrogen-related receptor alpha (ERRα) is a key regulator of intestinal homeostasis and protects against colitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAllan Tran,Charlotte Scholtes,Mario Songane,Claudia Champagne,Luc Galarneau,Marie-Pier Levasseur,Nassima Fodil,Catherine Rosa Dufour,Vincent Giguère,Maya SalehPubMed 34302001
- Journal of thrombosis and haemostasis : JTH 19:814-8212020Cellular fibronectin promotes deep vein thrombosis in diet-induced obese mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNirav Dhanesha,Manish Jain,Prakash Doddapattar,Anetta Undas,Anil K ChauhanPubMed 33300307
- Zoological research 41:621-6312020Role of neutrophil chemoattractant CXCL5 in SARS-CoV-2 infection-induced lung inflammatory innate immune response in an hACE2 transfection mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Liang,Heng Li,Jing Li,Ze-Ning Yang,Jia-Li Li,Hui-Wen Zheng,Yan-Li Chen,Hai-Jing Shi,Lei Guo,Long-Ding LiuPubMed 33045777
- Frontiers in cellular and infection microbiology 10:3282020Impairs Antibacterial Innate Immunity to Systemic Infections in Part Through Hemozoin-Bound Bioactive Molecules.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher L Harding,Nicolas F Villarino,Elena Valente,Evelin Schwarzer,Nathan W SchmidtPubMed 32714882
- mBio 10:2019Staphylococcus aureus Exploits the Host Apoptotic Pathway To Persist during Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVolker Winstel et. al.PubMed 31719177
- Journal of leukocyte biology 105:177-1862018Mannan-binding lectin deficiency exacerbates sterile liver injury in mice through enhancing hepatic neutrophil recruitment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Zhou,Junru Li,Yu Yu,Yan Liu,Huifang Li,Yunzhi Liu,Jun Wang,Liyun Zhang,Xiao Lu,Zhengliang Chen,Daming ZuoPubMed 30351498
- Leukemia 32:1435-14442018Tracking preleukemic cells in vivo to reveal the sequence of molecular events in radiation leukemogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTom Verbiest,Rosemary Finnon,Natalie Brown,Lourdes Cruz-Garcia,Paul Finnon,Grainne O'Brien,Eleanor Ross,Simon Bouffler,Cheryl L Scudamore,Christophe BadiePubMed 29556020
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2
Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| IP | Flow Cyt | IHC-P | FuncS | IHC-Fr | |
|---|---|---|---|---|---|
| Mouse | Expected | Expected | Expected | Expected | Expected |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
FuncS
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Alternative names
Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g
Recommended products
Anti-Ly6g [1A8] antibody (ab210204) is a rat monoclonal antibody that is provided in an azide free formulation. It is used to detect Ly6g in Flow Cytometry, IP, IHC-P, IHC-Fr, FuncS. Suitable for Mouse samples. Available in 5mg, 10mg, 25mg and 50mg sizes for in-vivo use upon request.
- Suitable for in vitro and in vivo studies, including neutralization, blocking, or activation/proliferation
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
pH: 7.2
Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- 1A8
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
- Purification notes
ab210204 was purified from tissue culture supernatant via affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free (ab210204) is a rat monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Functional Studies in Mouse samples.
Trusted by the scientific community
Anti-Ly6g [1A8] - Low endotoxin, Azide free (ab210204) was first used in a scientific publication in 2016 and has been cited over 10 times in peer-reviewed journals.
Other related products
We have a range of other formats of antibody clone [1A8] also available for your convenience:
PE/Cy7® Anti-Ly6g antibody [1A8] ab210206, PerCP/Cy5.5® Anti-Ly6g antibody [1A8] ab210207, violetFluor™ 450 - violetFluor™ 450 Anti-Ly6g antibody [1A8] ab242074, Carrier free - Anti-Ly6g antibody [1A8]- mouse IgG2a (Chimeric) -Low endotoxin, Azide free ab320707, Carrier free - Anti-Ly6g antibody [1A8]- mouse IgG2c (Chimeric)- Low endotoxin, Azide free ab320708
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ly6G also known as lymphocyte antigen 6 complex locus G6D 1A8 or 8C5 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein. The molecule has a molecular weight of about 25-30 kDa. Its expression is mainly on neutrophils and a subpopulation of eosinophils in mice. It plays a role in the innate immune system marking these immune cells for differentiation and response to infection. Researchers consider it a specific marker for detecting and isolating mouse neutrophils often using methods such as Ly6G staining or anti-Ly6G antibodies for identification.
- Biological function summary
Ly6G functions in the immune response by facilitating the migration and adhesion of neutrophils to the site of infection or injury. It is part of a protein complex on the cell surface that includes other Ly6 proteins. This group of proteins helps to modulate signal transduction for immune cell functions. Ly6G serves as an anchor through its GPI linkage aiding cell-to-cell communication and signal amplification in inflammation processes.
- Pathways
Ly6G is significantly integrated into the immune signaling pathways notably the toll-like receptor (TLR) signaling pathway. Through these pathways Ly6G influences neutrophil migration and activation. Closely associated proteins include CD11b and CD18 which interact with Ly6G in neutrophil functions including adhesion and transmigration. These pathways are essential for orchestrating the body's defense mechanisms against pathogens.
- Associated diseases and disorders
Ly6G plays an essential role in inflammatory conditions and autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of Ly6G can alter neutrophil behavior which contributes to disease pathology. Researchers are investigating the relationship between Ly6G and other immune-related proteins like myeloperoxidase (MPO) and elastase as these protein interactions may impact the development and progression of inflammatory diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry - Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free (ab210204), expandable thumbnail](https://content.abcam.com/products/images/ly6g-antibody-1a8-low-endotoxin-azide-free-ab210204--flow-cytometry-img445206.jpg "Flow Cytometry - Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free (ab210204)")
Flow Cytometry - Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free (ab210204)
Ly6g Flow Cytometry staining of Mouse bone marrow cells using rat Anti-Ly6g antibody
Flow cytometry staining of C57 BL/6 mouse bone marrow cells with ab320706 (right) or Rat IgG2a, kappa monoclonal (Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control ab18450), isotype control (left). Cells were incubated for 30 min on ice in 1x PBS containing 10% mouse serum and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab320706 or Rat IgG2a, kappa monoclonal (Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control ab18450), isotype control (1x 106 in 100 µl at 1.0 μg/ml ) for 30min on ice. The cells were simultaneously stained with APC anti-mouse Ly-6C Antibody.
The secondary antibody Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice.
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.
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