Anti-Ly6g antibody [1A8]- mouse IgG2c (Chimeric)- Low endotoxin, Azide free

• FuncS (Neut/Block)

AbReview6838155123974cb84faba584****

Functional Studies (Neut/Block) - Anti-Ly6g antibody [1A8]- mouse IgG2c (Chimeric)- Low endotoxin, Azide free (AB320708)

C57BL/6 WT mice were administered Anti-Ly6g antibody [1A8]- mouse IgG2c (Chimeric) -Low endotoxin, Azide free ab320708 and the corresponding isotype control ab280757 intraperitoneally at a dose of 200 μg per mouse every three days (72h). Peripheral blood was collected on days 3 and 6 to assess neutrophil depletion efficiency by gating CD11b+ Ly6G+ cells.

Figure A : Quantification of CD11b+ Ly6G+ cells in blood samples at different time point over the course treatment. Live cells CD45+ gated.

Figure B : Representative FACS plot of flow cytometric analysis of CD11b+ Ly6G+ over the course treatment.

This image is courtesy of an anonymous Abreview

• FuncS (Depl)

Supplier Data

Functional Studies (Depl) - Anti-Ly6g antibody [1A8]- mouse IgG2c (Chimeric)- Low endotoxin, Azide free (AB320708)

Flow cytometric analysis of peripheral blood samples from neutrophil Ly6G-Tomato reporter mice 48h post intraperitoneal injection with 500 µg of anti-Ly6G [1A8] antibody mouse IgG2c (chimeric) (ab320708) (A) or Anti-Ly6g [1A8] antibody – rat IgG2a (ab210204) (B) and corresponding isotype controls.

ab320708 injections result in prolonged neutrophils depletion compared to ab210204.

Cells were stained with CD45 conjugated to PE-Cy7 and CD11b conjugated to APC-eFluor 780. Gated on viable CD45+ population for analysis of CD11+ Ly6G-tdTomato+ neutrophils.

Developed in collaboration with Tatyana Chtanova lab, Garvan Institute of Medical Research and UNSW Sydney, Australia.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-Ly6g antibody [1A8]- mouse IgG2c (Chimeric)- Low endotoxin, Azide free (AB320708)

Flow cytometry staining of C57 BL/6 mouse bone marrow cells with ab320708 (right) or Mouse IgG2c, kappa monoclonal (ab170191), isotype control (left). Cells were incubated for 30 min on ice in 1x PBS containing 10%rat serum 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab320708 or Mouse IgG2c, kappa monoclonal (ab170191), isotype control (1x 10e6 in 100 μl at 1.0 μg/ml) for 30min on ice. The cells were simultaneously stained with APC anti-mouse Ly-6C Antibody.

The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice.

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.