Anti-Ly6g antibody [EPR22909-135] is a rabbit recombinant monoclonal antibody that is used to detect Ly6g in Flow cytometry, IHC-P. Suitable for Mouse samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Specificity and sensitivity confirmed in immunohistochemistry (IHC) with multi-tissue microarray (TMA) validation
- Cited in over 45 publications
Images
![Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (AB238132), expandable thumbnail](https://content.abcam.com/products/images/ly6g-antibody-epr22909-135-ab238132--flow-cytometry-img71323.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (AB238132)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (AB238132), expandable thumbnail](https://content.abcam.com/products/images/ly6g-antibody-epr22909-135-ab238132--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23155.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (AB238132)")
![Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (AB238132), expandable thumbnail](https://content.abcam.com/products/images/ly6g-antibody-epr22909-135-ab238132--flow-cytometry-img41593.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (AB238132)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (AB238132), expandable thumbnail](https://content.abcam.com/products/images/ly6g-antibody-epr22909-135-ab238132--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106953.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (AB238132)")
![Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (AB238132), expandable thumbnail](https://content.abcam.com/products/images/ly6g-antibody-epr22909-135-ab238132--flow-cytometry-img421242.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (AB238132)")
Publications
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- Frontiers in oncology 13:12868612023Loss of Cadherin-11 in pancreatic ductal adenocarcinoma alters tumor-immune microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAimy Sebastian,Kelly A Martin,Ivana Peran,Nicholas R Hum,Nicole F Leon,Beheshta Amiri,Stephen P Wilson,Matthew A Coleman,Elizabeth K Wheeler,Stephen W Byers,Gabriela G LootsPubMed 37954069
- Nature communications 14:59952023Tracing immune cells around biomaterials with spatial anchors during large-scale wound regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Yang,Chenyu Chu,Li Liu,Chenbing Wang,Chen Hu,Shengan Rung,Yi Man,Yili QuPubMed 37752124
- Cell communication and signaling : CCS 21:2502023Fasting-mimicking diet alleviates inflammatory pain by inhibiting neutrophil extracellular traps formation and neuroinflammation in the spinal cord.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Li,Ying Yue,Yan Ma,Ziwen Zhong,Miaomiao Guo,Jie Zhang,Zhiping Wang,Changhong MiaoPubMed 37735678
- Clinical and translational medicine 13:e13892023METTL3-mediated N6-methyladenosine exacerbates ferroptosis via m6A-IGF2BP2-dependent mitochondrial metabolic reprogramming in sepsis-induced acute lung injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Zhang,Dan Wu,Yanghanzhao Wang,Kefang Guo,Charles B Spencer,Lilibeth Ortoga,Mengdi Qu,Yuxin Shi,Yuwen Shao,Zhiping Wang,Juan P Cata,Changhong MiaoPubMed 37715457
- European journal of pharmacology 957:1760172023Alpha-1 antitrypsin protects against phosgene-induced acute lung injury by activating the ID1-dependent anti-inflammatory response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaihua He,Weihua Yu,Hongwei Li,Jiangzheng Liu,Yongmei Tu,Deqin Kong,Zi Long,Rui Liu,Jie Peng,Zhao Wang,Penghui Liu,Chunxu Hai,Wenjun Yan,Wenli LiPubMed 37673367
- International urology and nephrology 56:739-7492023JQ1 attenuates contrast-induced acute kidney injury through the upregulation of autophagy and inhibition of inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinghong Ge,Juntao Chen,Xueying Ren,Chunqi Huang,Danqing Dong,Zhou YinPubMed 37548899
- EBioMedicine 95:1047392023Neutrophil infiltration leads to fetal growth restriction by impairing the placental vasculature in DENV-infected pregnant mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingying Zhang,Ziyang Sheng,Qiaozhu Chen,Anni Zhou,Jiaying Cao,Feiyang Xue,Yanzhen Ye,Na Wu,Na Gao,Dongying Fan,Libo Liu,Yuetong Li,Peigang Wang,Li Liang,Deshan Zhou,Fuchun Zhang,Fang Li,Jing AnPubMed 37544202
- Journal of experimental & clinical cancer research : CR 42:1662023Pulmonary interleukin 1 beta/serum amyloid A3 axis promotes lung metastasis of hepatocellular carcinoma by facilitating the pre-metastatic niche formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChong Zhang,Qing Li,Qi Xu,Wei Dong,Chunmei Li,Bin Deng,Jiao Gong,Li-Zhen Zhang,Junfei JinPubMed 37443052
- Cell death & disease 14:4012023ATP-citrate lyase controls endothelial gluco-lipogenic metabolism and vascular inflammation in sepsis-associated organ injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRanran Li,Mei Meng,Ying Chen,Tingting Pan,Yinjiaozhi Li,Yunxin Deng,Ruyuan Zhang,Rui Tian,Wen Xu,Xiangtao Zheng,Fangchen Gong,Jie Liu,Haiting Tang,Xiaowei Ding,Yaoqing Tang,Djillali Annane,Erzhen Chen,Hongping Qu,Lei LiPubMed 37414769
- Frontiers in pharmacology 14:11937912023Single-cell RNA sequencing revealed subclonal heterogeneity and gene signatures of gemcitabine sensitivity in pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZelin Hou,Jiajing Lin,Yuan Ma,Haizhong Fang,Yuwei Wu,Zhijiang Chen,Xianchao Lin,Fengchun Lu,Shi Wen,Xunbin Yu,Heguang Huang,Yu PanPubMed 37324492
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Mouse | Not recommended | Tested | Not recommended | Not recommended | Tested |
| Transfected cell line | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Transfected cell line | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info 1/600 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/600 | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Transfected cell line | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Transfected cell line | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line | Dilution info - | Notes - |
Target data
Alternative names
Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g
Recommended products
Anti-Ly6g antibody [EPR22909-135] is a rabbit recombinant monoclonal antibody that is used to detect Ly6g in Flow cytometry, IHC-P. Suitable for Mouse samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Specificity and sensitivity confirmed in immunohistochemistry (IHC) with multi-tissue microarray (TMA) validation
- Cited in over 45 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR22909-135
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Ly6g antibody [EPR22909-135] (ab238132) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt, IHC-P in mouse samples.
Anti-Ly6g antibody [EPR22909-135] (ab238132) specifically detects Ly6g (UniProt ID: P35461; Molecular weight: 14kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Ly6g antibody [EPR22909-135] (ab238132) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Ly6g antibody [EPR22909-135] (ab238132) has been cited over 45 times in peer reviewed journals and is trusted by the scientific community.
Related Products
Conjugation-ready, carrier free format available for antibody clone EPR22909-135 - Anti-Ly6g antibody [EPR22909-135] - BSA and Azide free ab261916.
Antibody clone EPR22909-135 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, PE, APC, Alexa Fluor® 594, FITC (Alexa Fluor® 488 Anti-Ly6g antibody [EPR22909-135] ab283276, Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] ab303467, PE Anti-Ly6g antibody [EPR22909-135] ab303605, APC Anti-Ly6g antibody [EPR22909-135] ab303606, Alexa Fluor® 594 Anti-Ly6g antibody [EPR22909-135] ab307167, FITC Anti-Ly6g antibody [EPR22909-135] ab322293).
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ly6G also known as lymphocyte antigen 6 complex locus G6D 1A8 or 8C5 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein. The molecule has a molecular weight of about 25-30 kDa. Its expression is mainly on neutrophils and a subpopulation of eosinophils in mice. It plays a role in the innate immune system marking these immune cells for differentiation and response to infection. Researchers consider it a specific marker for detecting and isolating mouse neutrophils often using methods such as Ly6G staining or anti-Ly6G antibodies for identification.
- Biological function summary
Ly6G functions in the immune response by facilitating the migration and adhesion of neutrophils to the site of infection or injury. It is part of a protein complex on the cell surface that includes other Ly6 proteins. This group of proteins helps to modulate signal transduction for immune cell functions. Ly6G serves as an anchor through its GPI linkage aiding cell-to-cell communication and signal amplification in inflammation processes.
- Pathways
Ly6G is significantly integrated into the immune signaling pathways notably the toll-like receptor (TLR) signaling pathway. Through these pathways Ly6G influences neutrophil migration and activation. Closely associated proteins include CD11b and CD18 which interact with Ly6G in neutrophil functions including adhesion and transmigration. These pathways are essential for orchestrating the body's defense mechanisms against pathogens.
- Associated diseases and disorders
Ly6G plays an essential role in inflammatory conditions and autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of Ly6G can alter neutrophil behavior which contributes to disease pathology. Researchers are investigating the relationship between Ly6G and other immune-related proteins like myeloperoxidase (MPO) and elastase as these protein interactions may impact the development and progression of inflammatory diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132), expandable thumbnail](https://content.abcam.com/products/images/ly6g-antibody-epr22909-135-ab238132--flow-cytometry-img71323.jpg "Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)")
Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)
Flow cytometric analysis of mouse peripheral blood mononuclear cell (PBMC) cells labeling Ly6g with ab238132 at 1/600 dilution. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with isotype control rabbit monoclonal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (Left) or ab238132 (Right), then stained with anti-CD11b conjugated to BV421.
Gated on viable cells.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (ab238132), expandable thumbnail](https://content.abcam.com/products/images/ly6g-antibody-epr22909-135-ab238132--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23155.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (ab238132)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (ab238132)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Ly6g with ab238132 at 1/2000 dilution (0.32 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on neutrophils in mouse spleen (PMID: 21976773) is observed. The section was incubated with ab238132 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132), expandable thumbnail](https://content.abcam.com/products/images/ly6g-antibody-epr22909-135-ab238132--flow-cytometry-img41593.jpg "Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)")
Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)
Flow cytometric analysis of HEK-293T (Human embryonic kidney epithelial cell) transfected with HA-tagged Ly6c overexpression construct (Left) / HEK-293T transfected with HA-tagged Ly6g overexpression construct (Right) cells, labeling Ly6g with ab238132 at 1/600 dilution. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Ly6c (Left) and Ly6g (Right) overexpression cells were surface stained with ab238132 and anti-HA tag conjugated to Alexa Fluor®647.
ab238132 does not cross-react with Ly6c.
Gated on viable cells.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (ab238132), expandable thumbnail](https://content.abcam.com/products/images/ly6g-antibody-epr22909-135-ab238132--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106953.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (ab238132)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly6g antibody [EPR22909-135] (ab238132)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Ly6g with ab238132 at 1/2000 dilution (0.32 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on neutrophils in mouse lung (PMID: 22434870) is observed. The section was incubated with ab238132 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132), expandable thumbnail](https://content.abcam.com/products/images/ly6g-antibody-epr22909-135-ab238132--flow-cytometry-img421242.jpg "Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)")
Flow Cytometry - Anti-Ly6g antibody [EPR22909-135] (ab238132)
Flow cytometry staining of C57 BL/6 mouse bone marrow cells with ab238132 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab238132 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 106 in 100 µl at 0.04 μg/ml (1/58750)) for 30min on ice. The cells were simultaneously stained with CD11b.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.
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