AB123505

Anti-LY75/DEC-205 antibody [HD30]

Name: Anti-LY75/DEC-205 antibody [HD30] (ab123505) | Abcam
Brand: Abcam Limited
SKU: AB123505
Price: 440 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal LY75 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LY75.

View Alternative Names

CD205, CLEC13B, LY75, Lymphocyte antigen 75, Ly-75, C-type lectin domain family 13 member B, DEC-205, gp200-MR6

2 Images

Flow Cytometry - Anti-LY75/DEC-205 antibody [HD30] (AB123505)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-LY75/DEC-205 antibody [HD30] (AB123505)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab123505 (concentration in sample 0.6 μg/ml, GAM APC).

Flow Cyt

Supplier Data

Flow Cytometry - Anti-LY75/DEC-205 antibody [HD30] (AB123505)

Separation of human monocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab123505 (concentration in sample 0.6 μg/ml, GAM APC).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HD30

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human LY75.

O60449

Specificity

The mouse monoclonal antibody HD30 recognizes an extracellular epitope of CD205, an approx. 200 kDa Ctype lectin transmembrane protein of the MMR (macrophage mannose receptor) family, expressed at high levels on dendritic cells and thymic epithelial cells, and at low levels on lymphocytes, NK cells and monocytes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-4 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab123505 is purified from cell culture supernatant by protein A affinity chromatography. Purity is > 95% (by SDS-PAGE).

Storage buffer

pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein LY75 also known as DEC-205 is a type I transmembrane glycoprotein belonging to the C-type lectin receptor family. It has a molecular mass of approximately 205 kDa. LY75 is expressed mainly on dendritic cells macrophages and B lymphocytes playing a critical role in antigen uptake and processing. The receptor exhibits high-affinity binding to carbohydrate structures facilitating its function in the immune response.

Biological function summary

LY75/DEC-205 functions primarily in the immune system by mediating endocytosis of antigens for processing and presentation to T cells. It operates either alone or as part of a larger protein complex guiding immune surveillance and response. In particular it influences the maturation and activation of dendritic cells critical processes for initiating adaptive immunity.

Pathways

LY75/DEC-205 is integral to the antigen processing and presentation pathway interacting significantly with proteins like the major histocompatibility complex (MHC) molecules. The receptor also engages in the Toll-like receptor signaling pathway linking innate and adaptive immunity through its collaborations with these receptor proteins.

LY75/DEC-205 associates with autoimmune diseases and certain cancers. Its expression and function in dendritic cells implicate it in autoimmune conditions such as lupus where immune regulation is disrupted. It may also relate to tumor progression in cancers with an immune evasion component like melanoma. This connection involves proteins like PD-L1 which tumor cells express to escape immune recognition.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment (By similarity). Causes reduced proliferation of B-lymphocytes.

See full target information LY75

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cardiovascular medicine 10:990422 PubMed36895836

2023

Indoxyl sulfate induces left ventricular hypertrophy the AhR-FGF23-FGFR4 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroshi Kishimoto,Toshiaki Nakano,Kumiko Torisu,Masanori Tokumoto,Yushi Uchida,Shunsuke Yamada,Masatomo Taniguchi,Takanari Kitazono

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LY75/DEC-205 antibody [HD30]

Flow Cytometry - Anti-LY75/DEC-205 antibody [HD30] (AB123505)

Flow Cytometry - Anti-LY75/DEC-205 antibody [HD30] (AB123505)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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