Rabbit Recombinant Monoclonal LY75 antibody. Suitable for WB, Flow Cyt and reacts with Human, Mouse samples.
Images
![Flow Cytometry - Anti-LY75/DEC-205 antibody [EPR27222-93] (AB316000), expandable thumbnail](https://content.abcam.com/products/images/ly75-dec-205-antibody-epr27222-93-ab316000--flow-cytometry-img426359.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-LY75/DEC-205 antibody [EPR27222-93] (AB316000)")
![Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] (AB316000), expandable thumbnail](https://content.abcam.com/products/images/ly75-dec-205-antibody-epr27222-93-ab316000--western-blot-img426311.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] (AB316000)")
![Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] (AB316000), expandable thumbnail](https://content.abcam.com/products/images/ly75-dec-205-antibody-epr27222-93-ab316000--western-blot-img426310.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] (AB316000)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt | IP | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment. Causes reduced proliferation of B lymphocytes (By similarity).
Alternative names
CD205, Cd205, SMU_610, spaP, Lymphocyte antigen 75, Ly-75, DEC-205
Recommended products
Rabbit Recombinant Monoclonal LY75 antibody. Suitable for WB, Flow Cyt and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR27222-93
- Purification technique
- Affinity purification Protein A
- Specificity
Not suitable for FC-human.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry - Anti-LY75/DEC-205 antibody [EPR27222-93] (ab316000), expandable thumbnail](https://content.abcam.com/products/images/ly75-dec-205-antibody-epr27222-93-ab316000--flow-cytometry-img426359.jpg "Flow Cytometry - Anti-LY75/DEC-205 antibody [EPR27222-93] (ab316000)")
Flow Cytometry - Anti-LY75/DEC-205 antibody [EPR27222-93] (ab316000)
Flow cytometric analysis of Mouse splenocyte cells labelling LY75/DEC-205 with ab316000 at 1/500 dilution (0.1 ug)/Red and Blue compared with a isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Cells were stained with CD11c conjugated to Alexa Fluor® 647 and CD8a conjugated to Brilliant Violet 421. Gated on viable CD11c+ CD8a neg and CD11c+ CD8a+ populations.
![Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] (ab316000), expandable thumbnail](https://content.abcam.com/products/images/ly75-dec-205-antibody-epr27222-93-ab316000--western-blot-img426311.jpg "Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] (ab316000)")
Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] (ab316000)
Low expression: liver (PMID: 7753172).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 7553896).
The identity of the lower MW bands are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] (ab316000) at 1/1000 dilution
Lane 1: Human tonsil tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Human thymus tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Human liver tissue lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 250 kDa, 36 kDa
Exposure time: 59s
![Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] (ab316000), expandable thumbnail](https://content.abcam.com/products/images/ly75-dec-205-antibody-epr27222-93-ab316000--western-blot-img426310.jpg "Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] (ab316000)")
Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] (ab316000)
Low expression: liver, skeletal muscle (PMID: 7753172).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 7553896).
The identity of the lower MW bands are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] (ab316000) at 1/1000 dilution
Lane 1: Mouse lymph node tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Mouse thymus tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Mouse liver tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Mouse skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 205 kDa, 36 kDa
Exposure time: 59s
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