Rabbit Recombinant Monoclonal LY75 antibody. Suitable for WB, Flow Cyt and reacts with Human, Mouse samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | Flow Cyt | IP | IHC-P | ICC/IF | |
---|---|---|---|---|---|
Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Select an associated product type
Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment. Causes reduced proliferation of B lymphocytes (By similarity).
Lymphocyte antigen 75, Ly-75, DEC-205, SMU_610, spaP
Rabbit Recombinant Monoclonal LY75 antibody. Suitable for WB, Flow Cyt and reacts with Human, Mouse samples.
Lymphocyte antigen 75, Ly-75, DEC-205, SMU_610, spaP
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR27222-93
Affinity purification Protein A
Not suitable for FC-human.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Flow cytometric analysis of Mouse splenocyte cells labelling LY75/DEC-205 with ab316000 at 1/500 dilution (0.1 ug)/Red and Blue compared with a isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Cells were stained with CD11c conjugated to Alexa Fluor® 647 and CD8a conjugated to Brilliant Violet 421. Gated on viable CD11c+ CD8a neg and CD11c+ CD8a+ populations.
Low expression: liver (PMID: 7753172).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 7553896).
The identity of the lower MW bands are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] (ab316000) at 1/1000 dilution
Lane 1: Human tonsil tissue lysate at 20 µg
Lane 2: Human thymus tissue lysate at 20 µg
Lane 3: Human liver tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 250 kDa, 36 kDa
Exposure time: 59s
Low expression: liver, skeletal muscle (PMID: 7753172).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 7553896).
The identity of the lower MW bands are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] (ab316000) at 1/1000 dilution
Lane 1: Mouse lymph node tissue lysate at 20 µg
Lane 2: Mouse thymus tissue lysate at 20 µg
Lane 3: Mouse liver tissue lysate at 20 µg
Lane 4: Mouse skeletal muscle tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 205 kDa, 36 kDa
Exposure time: 59s
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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