Rabbit Recombinant Monoclonal LY75 antibody. Carrier free. Suitable for WB, Flow Cyt and reacts with Human, Mouse samples.
Images
![Flow Cytometry - Anti-LY75/DEC-205 antibody [EPR27222-93] - BSA and Azide free (AB316001), expandable thumbnail](https://content.abcam.com/products/images/ly75-dec-205-antibody-epr27222-93-bsa-and-azide-free-ab316001--flow-cytometry-img426359.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-LY75/DEC-205 antibody [EPR27222-93] - BSA and Azide free (AB316001)")
![Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] - BSA and Azide free (AB316001), expandable thumbnail](https://content.abcam.com/products/images/ly75-dec-205-antibody-epr27222-93-bsa-and-azide-free-ab316001--western-blot-img426311.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] - BSA and Azide free (AB316001)")
![Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] - BSA and Azide free (AB316001), expandable thumbnail](https://content.abcam.com/products/images/ly75-dec-205-antibody-epr27222-93-bsa-and-azide-free-ab316001--western-blot-img426310.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] - BSA and Azide free (AB316001)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt | IP | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment. Causes reduced proliferation of B lymphocytes (By similarity).
Alternative names
Lymphocyte antigen 75, Ly-75, DEC-205, SMU_610, spaP
Recommended products
Rabbit Recombinant Monoclonal LY75 antibody. Carrier free. Suitable for WB, Flow Cyt and reacts with Human, Mouse samples.
Alternative names
Lymphocyte antigen 75, Ly-75, DEC-205, SMU_610, spaP
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR27222-93
- Purification technique
Affinity purification Protein A
- Specificity
Not suitable for FC-human.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab316001 is the carrier-free version of Anti-LY75/DEC-205 antibody [EPR27222-93] ab316000.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein LY75 also known as DEC-205 is a type I transmembrane glycoprotein belonging to the C-type lectin receptor family. It has a molecular mass of approximately 205 kDa. LY75 is expressed mainly on dendritic cells macrophages and B lymphocytes playing a critical role in antigen uptake and processing. The receptor exhibits high-affinity binding to carbohydrate structures facilitating its function in the immune response.
- Biological function summary
LY75/DEC-205 functions primarily in the immune system by mediating endocytosis of antigens for processing and presentation to T cells. It operates either alone or as part of a larger protein complex guiding immune surveillance and response. In particular it influences the maturation and activation of dendritic cells critical processes for initiating adaptive immunity.
- Pathways
LY75/DEC-205 is integral to the antigen processing and presentation pathway interacting significantly with proteins like the major histocompatibility complex (MHC) molecules. The receptor also engages in the Toll-like receptor signaling pathway linking innate and adaptive immunity through its collaborations with these receptor proteins.
- Associated diseases and disorders
LY75/DEC-205 associates with autoimmune diseases and certain cancers. Its expression and function in dendritic cells implicate it in autoimmune conditions such as lupus where immune regulation is disrupted. It may also relate to tumor progression in cancers with an immune evasion component like melanoma. This connection involves proteins like PD-L1 which tumor cells express to escape immune recognition.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry - Anti-LY75/DEC-205 antibody [EPR27222-93] - BSA and Azide free (ab316001), expandable thumbnail](https://content.abcam.com/products/images/ly75-dec-205-antibody-epr27222-93-bsa-and-azide-free-ab316001--flow-cytometry-img426359.jpg "Flow Cytometry - Anti-LY75/DEC-205 antibody [EPR27222-93] - BSA and Azide free (ab316001)")
Flow Cytometry - Anti-LY75/DEC-205 antibody [EPR27222-93] - BSA and Azide free (ab316001)
This data was developed using Anti-LY75/DEC-205 antibody [EPR27222-93] ab316000, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Mouse splenocyte cells labelling LY75/DEC-205 with Anti-LY75/DEC-205 antibody [EPR27222-93] ab316000 at 1/500 dilution (0.1 ug)/Red and Blue compared with a isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Cells were stained with CD11c conjugated to Alexa Fluor® 647 and CD8a conjugated to Brilliant Violet 421. Gated on viable CD11c+ CD8a neg and CD11c+ CD8a+ populations.
![Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] - BSA and Azide free (ab316001), expandable thumbnail](https://content.abcam.com/products/images/ly75-dec-205-antibody-epr27222-93-bsa-and-azide-free-ab316001--western-blot-img426311.jpg "Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] - BSA and Azide free (ab316001)")
Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] - BSA and Azide free (ab316001)
This data was developed using Anti-LY75/DEC-205 antibody [EPR27222-93] ab316000, the same antibody clone in a different buffer formulation.
Low expression: liver (PMID: 7753172).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 7553896).
The identity of the lower MW bands are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] (Anti-LY75/DEC-205 antibody [EPR27222-93] ab316000) at 1/1000 dilution
Lane 1: Human tonsil tissue lysate at 20 µg
Lane 2: Human thymus tissue lysate at 20 µg
Lane 3: Human liver tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 250 kDa, 36 kDa
Exposure time: 59s
Low expression: liver (PMID: 7753172).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 7553896).
The identity of the lower MW bands are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
![Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] - BSA and Azide free (ab316001), expandable thumbnail](https://content.abcam.com/products/images/ly75-dec-205-antibody-epr27222-93-bsa-and-azide-free-ab316001--western-blot-img426310.jpg "Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] - BSA and Azide free (ab316001)")
Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] - BSA and Azide free (ab316001)
This data was developed using Anti-LY75/DEC-205 antibody [EPR27222-93] ab316000, the same antibody clone in a different buffer formulation.
Low expression: liver, skeletal muscle (PMID: 7753172).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 7553896).
The identity of the lower MW bands are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-LY75/DEC-205 antibody [EPR27222-93] (Anti-LY75/DEC-205 antibody [EPR27222-93] ab316000) at 1/1000 dilution
Lane 1: Mouse lymph node tissue lysate at 20 µg
Lane 2: Mouse thymus tissue lysate at 20 µg
Lane 3: Mouse liver tissue lysate at 20 µg
Lane 4: Mouse skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 205 kDa, 36 kDa
Exposure time: 59s
Low expression: liver, skeletal muscle (PMID: 7753172).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 7553896).
The identity of the lower MW bands are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
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