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Rabbit Recombinant Monoclonal LY75 antibody. Suitable for mIHC, IHC-P, WB and reacts with Human, Mouse samples. Cited in 3 publications.


Images

Western blot - Anti-LY75/DEC-205 antibody [EPR5233] (AB124897), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (AB124897), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (AB124897), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (AB124897), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-LY75/DEC-205 antibody [EPR5233] (AB124897), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCIHC-PWB
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1600
Notes

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

For unpurified use: 1/250.00000 - 1/500.00000

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/1600
Notes

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

For unpurified use: 1/250.00000 - 1/500.00000

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment (By similarity). Causes reduced proliferation of B-lymphocytes.

Alternative names

LY75, CD205, LY 75, Ly-75, gp200, CD 205, DEC205, CLEC13B, DEC 205, DEC-205, CLEC 13B, gp200 MR6, gp200-MR6, LY75_HUMAN, CD205 antigen, Lymphocyte antigen 75, Renal Cell Carcinoma (gp200), C-type lectin domain family 13 member B

Recommended products

Rabbit Recombinant Monoclonal LY75 antibody. Suitable for mIHC, IHC-P, WB and reacts with Human, Mouse samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5233
Purification technique
Affinity purification Protein A
Specificity

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Dissociation constant
2.54 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein LY75 also known as DEC-205 is a type I transmembrane glycoprotein belonging to the C-type lectin receptor family. It has a molecular mass of approximately 205 kDa. LY75 is expressed mainly on dendritic cells macrophages and B lymphocytes playing a critical role in antigen uptake and processing. The receptor exhibits high-affinity binding to carbohydrate structures facilitating its function in the immune response.

Biological function summary

LY75/DEC-205 functions primarily in the immune system by mediating endocytosis of antigens for processing and presentation to T cells. It operates either alone or as part of a larger protein complex guiding immune surveillance and response. In particular it influences the maturation and activation of dendritic cells critical processes for initiating adaptive immunity.

Pathways

LY75/DEC-205 is integral to the antigen processing and presentation pathway interacting significantly with proteins like the major histocompatibility complex (MHC) molecules. The receptor also engages in the Toll-like receptor signaling pathway linking innate and adaptive immunity through its collaborations with these receptor proteins.

Associated diseases and disorders

LY75/DEC-205 associates with autoimmune diseases and certain cancers. Its expression and function in dendritic cells implicate it in autoimmune conditions such as lupus where immune regulation is disrupted. It may also relate to tumor progression in cancers with an immune evasion component like melanoma. This connection involves proteins like PD-L1 which tumor cells express to escape immune recognition.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897), expandable thumbnail

    Western blot - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)

    DEC-205 is a 205 kD integral membrane protein abundant on dendritic cells in lymphoid tissues. We are unsure about the nature of the lower extra bands.

    All lanes: Western blot - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897) at 1/1000 dilution

    Lane 1: Human tonsil lysate at 20 µg

    Lane 2: Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

    Lane 3: Mouse lymph node lysate at 20 µg

    Lane 4: Mouse thymus lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 198 kDa

    Observed band size: 205 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human t cell lymphoma tissue sections labeling LY75/DEC-205 with Purified ab124897 at 1:1600 dilution (0.07 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thymus tissue sections labeling LY75/DEC-205 with Purified ab124897 at 1:1600 dilution (0.07 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling LY75/DEC-205 with Purified ab124897 at 1:1600 dilution (0.07 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

  • Multiplex immunohistochemistry - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897), expandable thumbnail

    Multiplex immunohistochemistry - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897)

    LY75/DEC-205 Multiplex immunohistochemistry staining of Human thymus using rabbit Anti-LY75/DEC-205 antibody

    This data was developed using Anti-LY75/DEC-205 antibody [EPR5233] - BSA and Azide free ab208649, the same antibody clone in a different buffer formulation.


    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human thymus tissue labeling CD3 epsilon with Anti-CD3 epsilon antibody [SP7] ab16669 at 1/500 dilution, LY75/DEC-205 with Anti-LY75/DEC-205 antibody [EPR5233] - BSA and Azide free ab208649 at 1/15000, and CD68 with Anti-CD68 antibody [EPR20545] ab213363 at 1/500 dilution.

    Panel A: merged staining of anti-CD68 (magenta; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-LY75/DEC-205 (red; Opal™570) on human thymus.
    Panel B: anti-CD3 epsilon stained on T cells.
    Panel C: anti-LY75/DEC-205 stained on thymic cortical epithelium and dendritic cells.
    Panel D: anti-CD68 stained on macrophages.

    Sections  were treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins before antibody incubation. The section was incubated in three rounds of staining: in the order of Anti-CD68 antibody [EPR20545] ab213363, Anti-CD3 epsilon antibody [SP7] ab16669, and Anti-LY75/DEC-205 antibody [EPR5233] - BSA and Azide free ab208649 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    DAPI was used as a nuclear counterstain.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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Product protocols

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