Anti-LY75/DEC-205 antibody [EPR5233]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (AB124897)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thymus tissue sections labeling LY75/DEC-205 with Purified ab124897 at 1 : 1600 dilution (0.07 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (AB124897)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human t cell lymphoma tissue sections labeling LY75/DEC-205 with Purified ab124897 at 1 : 1600 dilution (0.07 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LY75/DEC-205 antibody [EPR5233] (AB124897)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling LY75/DEC-205 with Purified ab124897 at 1 : 1600 dilution (0.07 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-LY75/DEC-205 antibody [EPR5233] (AB124897)

This data was developed using ab208649, the same antibody clone in a different buffer formulation. Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human thymus tissue labeling CD3 epsilon with ab16669 at 1/500 dilution, LY75/DEC-205 with ab208649 at 1/15000, and CD68 with ab213363 at 1/500 dilution. Panel A : merged staining of anti-CD68 (magenta; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-LY75/DEC-205 (red; Opal™570) on human thymus. Panel B : anti-CD3 epsilon stained on T cells. Panel C : anti-LY75/DEC-205 stained on thymic cortical epithelium and dendritic cells. Panel D : anti-CD68 stained on macrophages. Sections  were treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins before antibody incubation. The section was incubated in three rounds of staining : in the order of ab213363, ab16669, and ab208649 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. DAPI was used as a nuclear counterstain. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• WB

Unknown

Western blot - Anti-LY75/DEC-205 antibody [EPR5233] (AB124897)

DEC-205 is a 205 kD integral membrane protein abundant on dendritic cells in lymphoid tissues. We are unsure about the nature of the lower extra bands.

All lanes:

Western blot - Anti-LY75/DEC-205 antibody [EPR5233] (ab124897) at 1/1000 dilution

Lane 1:

Human tonsil lysate at 20 µg

Lane 2:

Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 3:

Mouse lymph node lysate at 20 µg

Lane 4:

Mouse thymus lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 198 kDa

Observed band size: 205 kDa

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