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AB233082

Anti-LYAR antibody

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(3 Publications )

Rabbit Polyclonal LYAR antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Lyar aa 250 to C-terminus.

View Alternative Names

PNAS-5, LYAR, Cell growth-regulating nucleolar protein

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYAR antibody (AB233082)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYAR antibody (AB233082)

Formalin-fixed, paraffin-embedded mouse testis tissue stained for LYAR using ab233082 at 20 μg/mL in immunohistochemical analysis. DAB staining.

Western blot - Anti-LYAR antibody (AB233082)
  • WB

Supplier Data

Western blot - Anti-LYAR antibody (AB233082)

All lanes:

Western blot - Anti-LYAR antibody (ab233082) at 3 µg/mL

All lanes:

Mouse placenta tissue lysate

Secondary

All lanes:

HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL

Predicted band size: 44 kDa

false

Western blot - Anti-LYAR antibody (AB233082)
  • WB

Supplier Data

Western blot - Anti-LYAR antibody (AB233082)

All lanes:

Western blot - Anti-LYAR antibody (ab233082) at 3 µg/mL

All lanes:

Rat testis tissue lysate

Secondary

All lanes:

HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL

Predicted band size: 44 kDa

false

Western blot - Anti-LYAR antibody (AB233082)
  • WB

Supplier Data

Western blot - Anti-LYAR antibody (AB233082)

All lanes:

Western blot - Anti-LYAR antibody (ab233082) at 3 µg/mL

All lanes:

Mouse testis tissue lysate

Secondary

All lanes:

HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL

Predicted band size: 44 kDa

false

Western blot - Anti-LYAR antibody (AB233082)
  • WB

Supplier Data

Western blot - Anti-LYAR antibody (AB233082)

All lanes:

Western blot - Anti-LYAR antibody (ab233082) at 3 µg/mL

All lanes:

A431 (human epidermoid carcinoma cell line) cell lysate

Secondary

All lanes:

HRP-Linked Goat anti-Rabbit IgG polyclonal at 0.2 µg/mL

Predicted band size: 44 kDa

false

Western blot - Anti-LYAR antibody (AB233082)
  • WB

Supplier Data

Western blot - Anti-LYAR antibody (AB233082)

All lanes:

Western blot - Anti-LYAR antibody (ab233082) at 2 µg/mL

All lanes:

Recombinant mouse LYAR protein

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Mouse Lyar aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q08288

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lyar also known as the cell growth-regulating nucleolar protein is a zinc finger protein with a molecular mass of approximately 36 kDa. This protein is expressed primarily in the nucleolus although it can also be found in other sub-nuclear structures. Lyar binds to nucleic acids and proteins playing roles in nucleolus-related processes. Its expression is seen in various tissues but it is most pronounced in rapidly dividing cells such as those in fetal tissues and certain cancers.
Biological function summary

The Lyar protein plays a role in regulating cell growth and proliferation. It participates in ribosome biogenesis by interacting with pre-ribosomal RNA possibly aiding in the stabilization and processing of rRNA precursors. Lyar is not usually considered as part of a protein complex but its interactions with several other nucleolar proteins imply its involvement in complex regulatory networks. It influences the transcription of ribosomal RNA genes impacting protein synthesis rates and cell cycle progression.

Pathways

Lyar is involved in the modulation of pathways linked to cell proliferation and survival. Notably it affects the PI3K/AKT signaling pathway by interacting with nucleophosmin (NPM1) impacting cell growth and survival mechanisms. This collaboration can alter the response of cells to external growth signals influencing cellular responses to metabolic changes and stress.

Aberrant Lyar levels have been implicated in oncogenesis. Its overexpression has been noted in various cancers including leukemia and neuroblastoma linking it to uncontrolled cell division and tumor progression. In cancer Lyar interacts with tumorigenesis-associated proteins such as c-Myc enhancing their transcriptional activity and contributing to cancerous transformation. Understanding the role of Lyar in these disease processes remains essential for identifying potential therapeutic strategies and developing targeted interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs (PubMed : 24495227). Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression (PubMed : 25092918). In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA (PubMed : 28686580). Preferentially binds to a DNA motif containing 5'-GGTTAT-3' (PubMed : 25092918). Negatively regulates the antiviral innate immune response by targeting IRF3 and impairing its DNA-binding activity (PubMed : 31413131). In addition, inhibits NF-kappa-B-mediated expression of pro-inflammatory cytokines (PubMed : 31413131). Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells (By similarity). Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal (By similarity).
See full target information LYAR
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Tissue engineering and regenerative medicine 22:57-75 PubMed39579168

2024

Development of Zinc-Containing Chitosan/Gelatin Coatings with Immunomodulatory Effect for Soft Tissue Sealing around Dental Implants.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Han,Jorine G F Sanders,Lea Andrée,Bart A J A van Oirschot,Adelina S Plachokova,Jeroen J J P van den Beucken,Sander C G Leeuwenburgh,Fang Yang

iScience 27:111084 PubMed39493878

2024

Exploring heparin's protective mechanism against AGEs induced endothelial injury.

Applications

Unspecified application

Species

Unspecified reactive species

Junfeng Shi,Yudong Guan,Hongwei Song,Liang Zhu,Jingjing Li,Qinying Li,Ningning Hou,Fang Han,Meng Wang,Kexin Zhang,Ming Shan,Xiaodong Sun,Hongyan Qiu

Brazilian journal of medical and biological resear 53:e9317 PubMed33111744

2020

LINC00355 promoted the progression of lung squamous cell carcinoma through regulating the miR-466/LYAR axis.

Applications

Unspecified application

Species

Unspecified reactive species

XueFeng Sun,GuangSuo Wang,PeiKun Ding,ShiXuan Li
View all publications
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