AB181261

Anti-LYAR antibody [EPR14352]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal LYAR antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PNAS-5, LYAR, Cell growth-regulating nucleolar protein

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-LYAR antibody [EPR14352] (AB181261)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LYAR antibody [EPR14352] (AB181261)

Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling LYAR with ab181261 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution and counter-stained with DAPI (blue).

Supplier Data

Western blot - Anti-LYAR antibody [EPR14352] (AB181261)

All lanes:

Western blot - Anti-LYAR antibody [EPR14352] (ab181261) at 1/10000 dilution

All lanes:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

Predicted band size: 44 kDa

false

Supplier Data

Western blot - Anti-LYAR antibody [EPR14352] (AB181261)

All lanes:

Western blot - Anti-LYAR antibody [EPR14352] (ab181261) at 1/20000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Ramos cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

Predicted band size: 44 kDa

false

Carrier free

Anti-LYAR antibody [EPR14352] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14352

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lyar also known as the cell growth-regulating nucleolar protein is a zinc finger protein with a molecular mass of approximately 36 kDa. This protein is expressed primarily in the nucleolus although it can also be found in other sub-nuclear structures. Lyar binds to nucleic acids and proteins playing roles in nucleolus-related processes. Its expression is seen in various tissues but it is most pronounced in rapidly dividing cells such as those in fetal tissues and certain cancers.

Biological function summary

The Lyar protein plays a role in regulating cell growth and proliferation. It participates in ribosome biogenesis by interacting with pre-ribosomal RNA possibly aiding in the stabilization and processing of rRNA precursors. Lyar is not usually considered as part of a protein complex but its interactions with several other nucleolar proteins imply its involvement in complex regulatory networks. It influences the transcription of ribosomal RNA genes impacting protein synthesis rates and cell cycle progression.

Pathways

Lyar is involved in the modulation of pathways linked to cell proliferation and survival. Notably it affects the PI3K/AKT signaling pathway by interacting with nucleophosmin (NPM1) impacting cell growth and survival mechanisms. This collaboration can alter the response of cells to external growth signals influencing cellular responses to metabolic changes and stress.

Aberrant Lyar levels have been implicated in oncogenesis. Its overexpression has been noted in various cancers including leukemia and neuroblastoma linking it to uncontrolled cell division and tumor progression. In cancer Lyar interacts with tumorigenesis-associated proteins such as c-Myc enhancing their transcriptional activity and contributing to cancerous transformation. Understanding the role of Lyar in these disease processes remains essential for identifying potential therapeutic strategies and developing targeted interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs (PubMed : 24495227). Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression (PubMed : 25092918). In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA (PubMed : 28686580). Preferentially binds to a DNA motif containing 5'-GGTTAT-3' (PubMed : 25092918). Negatively regulates the antiviral innate immune response by targeting IRF3 and impairing its DNA-binding activity (PubMed : 31413131). In addition, inhibits NF-kappa-B-mediated expression of pro-inflammatory cytokines (PubMed : 31413131). Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells (By similarity). Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal (By similarity).

See full target information LYAR

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 18:4239-4246 PubMed30221720

2018

Long non‑coding RNA ferritin heavy polypeptide 1 pseudogene 3 controls glioma cell proliferation and apoptosis via regulation of the microRNA‑224‑5p/tumor protein D52 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqiang Zhang,Ying Li,Jing Wang,Ping Lei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LYAR antibody [EPR14352]

Immunocytochemistry/ Immunofluorescence - Anti-LYAR antibody [EPR14352] (AB181261)

Western blot - Anti-LYAR antibody [EPR14352] (AB181261)

Western blot - Anti-LYAR antibody [EPR14352] (AB181261)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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