Rabbit Recombinant Monoclonal XCL1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-Lymphotactin/ATAC antibody [EPR20426-92] - BSA and Azide free (Capture) (AB244607), expandable thumbnail](https://content.abcam.com/products/images/lymphotactin-atac-antibody-epr20426-92-bsa-and-azide-free-capture-ab244607--sandwich-elisa-img52196.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Lymphotactin/ATAC antibody [EPR20426-92] - BSA and Azide free (Capture) (AB244607)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Chemotactic activity for lymphocytes but not for monocytes or neutrophils. In thymus, mediates medullary accumulation of thymic dendritic cells and contributes to regulatoy T cell development, playing a role in self-tolerance establishment.
Alternative names
LTN, SCYC1, XCL1, Lymphotactin, ATAC, C motif chemokine 1, Cytokine SCM-1, Lymphotaxin, SCM-1-alpha, Small-inducible cytokine C1, XC chemokine ligand 1
Recommended products
Rabbit Recombinant Monoclonal XCL1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20426-92
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab244607 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human XCL1. The recommended pair for sandwich ELISA is:
Capture: ab244607, Human XCL1 Capture Antibody (unconjugated)
Detector: Anti-Lymphotactin/ATAC antibody [EPR20426-4] - BSA and Azide free (Detector) ab244857, Human XCL1 Detector Antibody (unconjugated)
The reference range value is 97.48 - 2800 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lymphotactin also known as ATAC (Activation-induced T cell-derived and chemokine related cytokine) is a chemokine that plays an important role in immune system communication. It has a molecular mass of around 12 kDa. Lymphotactin is unique because it is the only member of the C class chemokines lacking one of the conserved cysteines found in other chemokines. It is primarily expressed in activated T cells and NK cells and can be found in tissues such as the spleen thymus and lymph nodes.
- Biological function summary
This chemokine specifically attracts T cells and NK cells to sites of inflammation. It is not known to form part of larger molecular complexes. Lymphotactin contributes to cell signaling events that direct immune cells to infection sites or areas of tissue damage ensuring specific and effective immune responses. This targeting is especially useful during the immune system’s response to pathogens aiding in the clearance of infections.
- Pathways
Lymphotactin influences immune response pathways especially those involved in cell-mediated responses. It interacts with the chemokine receptor XCR1 which plays a role in immune surveillance by guiding T cells to infected zones. Lymphotactin's activity bridges innate and adaptive immunity by directing cells where they are needed most linking it to pathways like cytokine signaling and inflammatory response.
- Associated diseases and disorders
Lymphotactin has a role in conditions such as inflammatory bowel disease and certain autoimmune disorders. Overproduction or dysregulation can exacerbate these conditions by leading to excessive immune cell infiltration and inflammation. The chemokine connects to proteins like XCR1 in these disease contexts influencing cellular migration and the extent of an inflammatory response. Understanding its disease-related interactions helps in the development of targeted therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-Lymphotactin/ATAC antibody [EPR20426-92] - BSA and Azide free (Capture) (ab244607), expandable thumbnail](https://content.abcam.com/products/images/lymphotactin-atac-antibody-epr20426-92-bsa-and-azide-free-capture-ab244607--sandwich-elisa-img52196.jpg "Sandwich ELISA - Anti-Lymphotactin/ATAC antibody [EPR20426-92] - BSA and Azide free (Capture) (ab244607)")
Sandwich ELISA - Anti-Lymphotactin/ATAC antibody [EPR20426-92] - BSA and Azide free (Capture) (ab244607)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Human XCL1 ELISA Kit ab264620).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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