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Rabbit Recombinant Monoclonal LYPD3 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 4 publications.

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Images

Western blot - Anti-LYPD3 antibody [EPR9107] (AB151709), expandable thumbnail
  • Immunoprecipitation - Anti-LYPD3 antibody [EPR9107] (AB151709), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] (AB151709), expandable thumbnail
  • Western blot - Anti-LYPD3 antibody [EPR9107] (AB151709), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] (AB151709), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWB
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

For unpurified use at 1/100 - 1/250.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/10 - 1/100

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Supports cell migration. May be involved in urothelial cell-matrix interactions. May be involved in tumor progression.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal LYPD3 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 4 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR9107

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

LYPD3 also known as C4.4A is a glycosylphosphatidylinositol (GPI)-anchored protein with a molecular weight of approximately 40 kDa. It belongs to the Ly6/PLAUR domain family. This protein is mainly expressed in stratified epithelia and also found in some other tissues. LYPD3 shows higher expression levels in certain cancer cell types indicating its role in tumorigenesis.

Biological function summary

LYPD3 functions in cellular adhesion and migration processes. It interacts with extracellular matrix components facilitating the movement and positioning of cells. It acts as a part of a protein complex that is important for cell communication and tissue organization. Its high expression in some cancer types suggests its involvement in processes that drive cancer progression including metastasis.

Pathways

LYPD3 participates in key signaling cascades including integrin-mediated pathways. These pathways are important for mediating cell-matrix interactions and influence cell movement and survival. LYPD3 has known interactions with integrin proteins suggesting a collaborative role that modulates cell adhesion dynamics. These interactions make it an important player in cell migration and invasion processes that are pivotal for normal physiological events and pathological conditions.

Associated diseases and disorders

LYPD3 shows significant involvement in cancers such as squamous cell carcinoma and prostate cancer. The protein aids the metastatic spread of these cancers by promoting cell migration and invasion. It has been associated with other proteins like laminin which supports cancer cell detachment and dissemination. Understanding LYPD3's role in cancer biology helps in identifying therapeutic targets for impeding cancer progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Western blot - Anti-LYPD3 antibody [EPR9107] (ab151709), expandable thumbnail

    Western blot - Anti-LYPD3 antibody [EPR9107] (ab151709)

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-LYPD3 antibody [EPR9107] (ab151709) at 1/1000 dilution

    Lane 1: HaCaT (Human skin keratinocyte) whole cell lysate at 20 µg

    Lane 2: BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: Human esophagus tissue lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/5000 dilution

    Predicted band size: 36 kDa

  • Immunoprecipitation - Anti-LYPD3 antibody [EPR9107] (ab151709), expandable thumbnail

    Immunoprecipitation - Anti-LYPD3 antibody [EPR9107] (ab151709)

    ab151709 (purified) at 1:50 dilution (2ug) immunoprecipitating LYPD3 in HaCaT (Human skin keratinocyte) whole cell lysate.
    Lane 1 (input): HaCaT (Human skin keratinocyte) whole cell lysate 10ug
    Lane 2 (+): ab151709 & HaCaT (Human skin keratinocyte) whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab151709 in HaCaT (Human skin keratinocyte) whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-LYPD3 antibody [EPR9107] (ab151709)

    Predicted band size: 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] (ab151709), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] (ab151709)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue sections labeling LYPD3 with Purified ab151709 at 1:1000 dilution (1.01 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-LYPD3 antibody [EPR9107] (ab151709), expandable thumbnail

    Western blot - Anti-LYPD3 antibody [EPR9107] (ab151709)

    All lanes: Western blot - Anti-LYPD3 antibody [EPR9107] (ab151709) at 1/1000 dilution

    Lane 1: BxPC3 cell lysate at 10 µg

    Lane 2: HACAT cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] (ab151709), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYPD3 antibody [EPR9107] (ab151709)

    Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling LYPD3 with unpurified ab151709 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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