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AB124789

Anti-LYRIC/AEG1 antibody [EP4445]

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(19 Publications)

Rabbit Recombinant Monoclonal LYRIC/AEG1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 19 publications.

View Alternative Names

AEG1, LYRIC, MTDH, Protein LYRIC, 3D3/LYRIC, Astrocyte elevated gene-1 protein, Lysine-rich CEACAM1 co-isolated protein, Metadherin, Metastasis adhesion protein, AEG-1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EP4445] (AB124789)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EP4445] (AB124789)

ab124789 at 1/100 dilution staining LYRIC/AEG1 in paraffin-embedded Human breast tissues by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-LYRIC/AEG1 antibody [EP4445] (AB124789)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-LYRIC/AEG1 antibody [EP4445] (AB124789)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LYRIC/AEG1 with purified ab124789 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

Control : PBS only.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EP4445] (AB124789)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EP4445] (AB124789)

ab124789 at 1/100 dilution staining LYRIC/AEG1 in paraffin-embedded Human brain tissues by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-LYRIC/AEG1 antibody [EP4445] (AB124789)
  • WB

Unknown

Western blot - Anti-LYRIC/AEG1 antibody [EP4445] (AB124789)

All lanes:

Western blot - Anti-LYRIC/AEG1 antibody [EP4445] (ab124789) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

PC3 cell lysate at 10 µg

Lane 4:

PC12 cell lysate at 10 µg

Lane 5:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 64 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP4445

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p>Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LYRIC/AEG1 also known as Astrocyte Elevated Gene-1 (AEG-1) is a protein with a molecular weight of approximately 60 kDa. This protein is expressed in several tissues with notable expression in the brain liver and other organs. LYRIC/AEG1 is encoded by the MTDH gene and plays a mechanical role in regulating processes such as gene expression and cell survival. It localizes to the endoplasmic reticulum the perinuclear region and also the cell nucleus indicating a multifaceted role in cellular dynamics.
Biological function summary

LYRIC/AEG1 functions significantly in oncogenesis and tumor progression. It participates actively in gene regulation and interacts with various proteins as part of complex protein networks influencing cell survival migration and adherence. This protein is known to enhance metastasis and promote angiogenesis in malignant contexts. Researchers often study the modulation of LYRIC/AEG1 to assess its impact on cell proliferation and differentiation in cancer cells.

Pathways

LYRIC/AEG1 is associated with important signaling pathways such as the NF-κB and PI3K/Akt pathways. These pathways are critical for cell survival and proliferation and LYRIC/AEG1 modulates their activity. Through these pathways it interacts with other proteins like IκB kinase complexes and Akt coordinating cellular responses to external stimuli. LYRIC/AEG1’s influence on these pathways highlights its role in cellular adaptation to stress and potential therapeutic interventions.

LYRIC/AEG1’s role becomes apparent in its association with cancers specifically hepatocellular carcinoma and breast cancer. In these cancers LYRIC/AEG1 overexpression correlates with poor prognosis and enhanced tumor progression. Its interaction with proteins such as c-Myc and E-cadherin highlights its involvement in cancer cell metabolism and epithelial-mesenchymal transition (EMT). Understanding LYRIC/AEG1’s function in disease contexts aids in developing targeted treatment strategies for these malignancies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Down-regulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
See full target information MTDH
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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Genomics, proteomics & bioinformatics 21:1246-1259 PubMed37121272

2023

The Proteome Landscape of Human Placentas for Monochorionic Twins with Selective Intrauterine Growth Restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Lu Meng,Peng-Bo Yuan,Xue-Ju Wang,Jing Hang,Xiao-Ming Shi,Yang-Yu Zhao,Yuan Wei

International journal of molecular sciences 24: PubMed36902125

2023

Metadherin Regulates Inflammatory Breast Cancer Invasion and Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriela Ortiz-Soto,Natalia S Babilonia-Díaz,Mercedes Y Lacourt-Ventura,Delmarie M Rivera-Rodríguez,Jailenne I Quiñones-Rodríguez,Mónica Colón-Vargas,Israel Almodóvar-Rivera,Luis E Ferrer-Torres,Ivette J Suárez-Arroyo,Michelle M Martínez-Montemayor

Molecular medicine reports 26: PubMed36177898

2022

LOC102553417 silencing facilitates the apoptosis of hepatic stellate cells via the miR‑30e/MTDH axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wujun Wei,Cheng Lin,Rentong Hu,Jingjing Huang,Xiaohao Chen,Lv Zhou,Jiazhu Wei,Yi-Bin Deng,Chun-Fang Wang

Proceedings of the National Academy of Sciences of the United States of America 119:e2203738119 PubMed36067288

2022

Miat and interacting protein Metadherin maintain a stem-like niche to promote medulloblastoma tumorigenesis and treatment resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Kai-Lin Peng,Harish N Vasudevan,Dennis T Lockney,Rachel Baum,Ronald C Hendrickson,David R Raleigh,Adam M Schmitt

Cancer management and research 12:8363-8377 PubMed32982430

2020

Long Non-Coding RNA DARS-AS1 Contributes to Prostate Cancer Progression Through Regulating the MicroRNA-628-5p/MTDH Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Fan,Junhui Hou,Siqing Liu,Zuomin Xiao,Jia Cui

Cancer management and research 12:2947-2960 PubMed32431541

2020

Long Noncoding RNA DLGAP1-AS1 Promotes the Aggressive Behavior of Gastric Cancer by Acting as a ceRNA for microRNA-628-5p and Raising Astrocyte Elevated Gene 1 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jiying Deng,Qin Zhang,Lianwei Lu,Chunxia Fan

Cell death & disease 11:221 PubMed32251289

2020

mPGES-1/PGE2 promotes the growth of T-ALL cells in vitro and in vivo by regulating the expression of MTDH via the EP3/cAMP/PKA/CREB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqing Li,Jiaoting Chen,Wenjuan Yang,Hongyun Liu,Jieyu Wang,Jie Xiao,Shuangfeng Xie,Liping Ma,Danian Nie

International journal of molecular sciences 20: PubMed31698701

2019

AEG-1/miR-221 Axis Cooperatively Regulates the Progression of Hepatocellular Carcinoma by Targeting PTEN/PI3K/AKT Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Maheshkumar Kannan,Sridharan Jayamohan,Rajesh Kannan Moorthy,Siva Chander Chabattula,Mathan Ganeshan,Antony Joseph Velanganni Arockiam

Frontiers in oncology 9:847 PubMed31552174

2019

Dysregulation of miR-375/AEG-1 Axis by Human Papillomavirus 16/18-E6/E7 Promotes Cellular Proliferation, Migration, and Invasion in Cervical Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sridharan Jayamohan,Maheshkumar Kannan,Rajesh Kannan Moorthy,Nirmal Rajasekaran,Hun Soon Jung,Young Kee Shin,Antony Joseph Velanganni Arockiam

Oncology letters 17:4514-4520 PubMed30988817

2019

Metadherin overexpression in perihilar cholangiocarcinoma is associated with lymph node metastasis and poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Zhi Zhu,Ke Zhou,Lin-Lin Ruan,Fu Sun,Gen Wang,Wen-Fang Li
View all publications
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