AB239999

Anti-LYRIC/AEG1 antibody [EP4445] - BSA and Azide free

Name: Anti-LYRIC/AEG1 antibody [EP4445] - BSA and Azide free (ab239999) | Abcam
Brand: Abcam Limited
SKU: AB239999
Price: 905 USD
Availability: InStock

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal LYRIC/AEG1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

AEG1, LYRIC, MTDH, Protein LYRIC, 3D3/LYRIC, Astrocyte elevated gene-1 protein, Lysine-rich CEACAM1 co-isolated protein, Metadherin, Metastasis adhesion protein, AEG-1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EP4445] - BSA and Azide free (AB239999)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EP4445] - BSA and Azide free (AB239999)

ab124789 at 1/100 dilution staining LYRIC/AEG1 in paraffin-embedded Human brain tissues by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124789).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EP4445] - BSA and Azide free (AB239999)

ab124789 at 1/100 dilution staining LYRIC/AEG1 in paraffin-embedded Human breast tissues by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124789).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-LYRIC/AEG1 antibody [EP4445] - BSA and Azide free (AB239999)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-LYRIC/AEG1 antibody [EP4445] - BSA and Azide free (AB239999)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LYRIC/AEG1 with purified ab124789 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

Control : PBS only.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124789).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP4445

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "(heat to 98 degrees C, allow to cool for 10-20 minutes)", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab239999 is the carrier-free version of ab124789.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LYRIC/AEG1 also known as Astrocyte Elevated Gene-1 (AEG-1) is a protein with a molecular weight of approximately 60 kDa. This protein is expressed in several tissues with notable expression in the brain liver and other organs. LYRIC/AEG1 is encoded by the MTDH gene and plays a mechanical role in regulating processes such as gene expression and cell survival. It localizes to the endoplasmic reticulum the perinuclear region and also the cell nucleus indicating a multifaceted role in cellular dynamics.

Biological function summary

LYRIC/AEG1 functions significantly in oncogenesis and tumor progression. It participates actively in gene regulation and interacts with various proteins as part of complex protein networks influencing cell survival migration and adherence. This protein is known to enhance metastasis and promote angiogenesis in malignant contexts. Researchers often study the modulation of LYRIC/AEG1 to assess its impact on cell proliferation and differentiation in cancer cells.

Pathways

LYRIC/AEG1 is associated with important signaling pathways such as the NF-κB and PI3K/Akt pathways. These pathways are critical for cell survival and proliferation and LYRIC/AEG1 modulates their activity. Through these pathways it interacts with other proteins like IκB kinase complexes and Akt coordinating cellular responses to external stimuli. LYRIC/AEG1’s influence on these pathways highlights its role in cellular adaptation to stress and potential therapeutic interventions.

LYRIC/AEG1’s role becomes apparent in its association with cancers specifically hepatocellular carcinoma and breast cancer. In these cancers LYRIC/AEG1 overexpression correlates with poor prognosis and enhanced tumor progression. Its interaction with proteins such as c-Myc and E-cadherin highlights its involvement in cancer cell metabolism and epithelial-mesenchymal transition (EMT). Understanding LYRIC/AEG1’s function in disease contexts aids in developing targeted treatment strategies for these malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Down-regulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.

See full target information MTDH

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell division 20:22 PubMed41039618

2025

FGF4 drives tumor progression in triple-negative breast cancer via IL6/STAT3-mediated macrophage M2 polarization and immune suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanhe Zhang,Shushan Zhang,Yuanyuan Han

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LYRIC/AEG1 antibody [EP4445] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EP4445] - BSA and Azide free (AB239999)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRIC/AEG1 antibody [EP4445] - BSA and Azide free (AB239999)

Immunocytochemistry/ Immunofluorescence - Anti-LYRIC/AEG1 antibody [EP4445] - BSA and Azide free (AB239999)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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