JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB253001

Anti-LYRM4 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal LYRM4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LYRM4 aa 1 to C-terminus.

View Alternative Names

C6orf149, ISD11, CGI-203, LYRM4, LYR motif-containing protein 4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRM4 antibody (AB253001)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRM4 antibody (AB253001)

Paraffin-embedded human hippocampus tissue stained for LYRM4 using ab253001 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-LYRM4 antibody (AB253001)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LYRM4 antibody (AB253001)

PFA-fixed, Triton X-100 permeabilized U-251MG (Human brain glioma cell line) cells stained for LYRM4 (green) using ab253001 at 4 μg/ml in ICC/IF.

Western blot - Anti-LYRM4 antibody (AB253001)
  • WB

Unknown

Western blot - Anti-LYRM4 antibody (AB253001)

All lanes:

Western blot - Anti-LYRM4 antibody (ab253001) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate

Lane 2:

LYRM4 overexpression HEK-293T lysate

Predicted band size: 11 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human LYRM4 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9HD34

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LYRM4 also known as ISD11 is a mitochondrial protein with a mass of approximately 11 kDa. Mechanically LYRM4 plays a role in the regulation of mitochondrial metabolic activities specifically assisting in the maturation of the iron-sulfur (Fe-S) clusters important for respiratory function. LYRM4 is expressed in tissues with high mitochondrial activity such as muscle and liver. It forms a complex with the mitochondrial protein NFS1 enhancing its stability and functionality.
Biological function summary

LYRM4 associates with processes vital for cell metabolic homoeostasis. It forms part of the mitochondrial iron-sulfur cluster assembly machinery a complex essential for synthesizing Fe-S clusters. These clusters are required for numerous cellular functions including electron transport and enzyme activities. LYRM4 works closely with other components like ISCU and FXN helping to provide functional clusters to mitochondrial enzymes.

Pathways

LYRM4 functions within the context of mitochondrial iron-sulfur cluster biosynthesis and electron transport chain pathways. These pathways are critical for energy production and cellular metabolism. LYRM4 relates to proteins such as NFS1 and ISCU through these pathways facilitating the proper assembly and delivery of Fe-S clusters needed to maintain oxidative phosphorylation and cellular respiration.

LYRM4 mutations or dysfunctions relate to conditions such as mitochondrial myopathy and Friedreich's ataxia. These disorders involve impaired mitochondrial function and oxidative stress which stem from deficiencies in Fe-S cluster formation and function. The protein’s interactions with others like FXN implicated in Friedreich's ataxia highlight its importance in mitochondrial health and disease.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Stabilizing factor, of the core iron-sulfur cluster (ISC) assembly complex, that regulates, in association with NDUFAB1, the stability and the cysteine desulfurase activity of NFS1 and participates in the [2Fe-2S] clusters assembly on the scaffolding protein ISCU (PubMed : 17331979, PubMed : 31664822). The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1 : LYRM4 : NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (By similarity). May also participates in the iron-sulfur protein biogenesis in the cytoplasm through its interaction with the cytoplasmic form of NFS1 (PubMed : 19454487).
See full target information LYRM4
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Orphanet journal of rare diseases 20:453 PubMed40849475

2025

Alpha-lipoic acid supplementation improves pathological alterations in cellular models of Friedreich ataxia.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Talaverón-Rey,Diana Reche-López,Suleva Povea-Cabello,Mónica Álvarez-Córdoba,David Gómez-Fernández,Ana Romero-González,Paula Cilleros-Holgado,José Manuel Romero-Domínguez,Alejandra López-Cabrera,Rocío Piñero-Pérez,Susana González-Granero,José Manuel García-Verdugo,José A Sánchez-Alcázar

Antioxidants (Basel, Switzerland) 14: PubMed40002401

2025

Polydatin and Nicotinamide Prevent Iron Accumulation and Lipid Peroxidation in Cellular Models of Mitochondrial Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Cilleros-Holgado,David Gómez-Fernández,Rocío Piñero-Pérez,José Manuel Romero-Domínguez,Diana Reche-López,Mónica Álvarez-Córdoba,Ana Romero-González,Alejandra López-Cabrera,Marta Castro De Oliveira,Andrés Rodríguez-Sacristán,Susana González-Granero,José Manuel García-Verdugo,José Antonio Sánchez-Alcázar
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB254715

Anti-ZNF503 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF503 antibody (AB254715)

  • 0
  • 0
Primary Antibodies

AB254654

Anti-ZFHX4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ZFHX4 antibody (AB254654)

  • 0
  • 0
Primary Antibodies

AB231263

Anti-ZBTB38 antibody

Western blot - Anti-ZBTB38 antibody (AB231263)

  • 0
  • 0
Primary Antibodies

AB253017

Anti-HIP14 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIP14 antibody (AB253017)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com