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AB126379

Anti-LYRM5 antibody

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(2 Publications )

Rabbit Polyclonal LYRM5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ETFRF1 aa 1 to C-terminus.

View Alternative Names

LYRM5, ETFRF1, Electron transfer flavoprotein regulatory factor 1, LYR motif-containing protein 5

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRM5 antibody (AB126379)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRM5 antibody (AB126379)

Immunohistochemical analysis of human stomach tissue labeling LYRM5 in the cytoplasm of glandular cells with ab126379 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-LYRM5 antibody (AB126379)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LYRM5 antibody (AB126379)

Immunocytochemical analysis of RH-30 (Human bone marrow metastasis cell line) whole cells labeling LYRM5 in the microtubule organizing center with ab126379 at 2 μg/ml.

Western blot - Anti-LYRM5 antibody (AB126379)
  • WB

Supplier Data

Western blot - Anti-LYRM5 antibody (AB126379)

All lanes:

Western blot - Anti-LYRM5 antibody (ab126379) at 1/100 dilution

Lane 1:

Negative control (vector only transfected) - HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

LYRM5 over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)

Predicted band size: 11 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ETFRF1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6IPR1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LYRM5 protein also known as LYR motif containing 5 is a small protein with a mass of approximately 7 kDa. Researchers have identified its expression predominantly in mitochondrial regions where it plays a critical role in mitochondrial function. This protein forms part of the LYR family which is involved in various cellular activities. LYRM5's location in mitochondria suggests its direct involvement in mitochondrial processes and homeostasis.
Biological function summary

LYRM5 functions as an important component in mitochondrial bioenergetics. It associates with the complex known as NADH:ubiquinone oxidoreductase (Complex I) of the mitochondrial respiratory chain. Through this association LYRM5 supports the assembly and stabilization of Complex I a critical step in ATP production. This function highlights its importance in cellular energy metabolism emphasizing LYRM5’s role in maintaining proper mitochondrial function.

Pathways

LYRM5 is actively engaged in the oxidative phosphorylation pathway which is important for ATP generation in cells. It facilitates interactions within Complex I ensuring the efficient transfer of electrons in the electron transport chain. Additionally LYRM5 interacts with proteins such as NDUFS6 (part of Complex I) further illustrating its role in this vital energy-producing pathway.

Alterations or malfunctions of LYRM5 have been implicated in mitochondrial diseases often characterized by defects in energy production. Specifically studies link mutations in LYRM5 to disorders like Leigh syndrome a severe neurological condition. The protein's interaction with Complex I proteins like NDUFS6 also associates it with these mitochondrial pathologies suggesting a critical disease-related role for LYRM5 in maintaining mitochondrial integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a regulator of the electron transfer flavoprotein by promoting the removal of flavin from the ETF holoenzyme (composed of ETFA and ETFB).
See full target information ETFRF1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadp4765 PubMed39841844

2025

WWC proteins-mediated compensatory mechanism restricts schwannomatosis driven by loss of function.

Applications

Unspecified application

Species

Unspecified reactive species

Xueying Wang,Rui Zhu,Pengcheng Yu,Sixian Qi,Zhenxing Zhong,Ruxin Jin,Yu Wang,Yuan Gu,Dan Ye,Kang Chen,Yilai Shu,Yi Wang,Fa-Xing Yu

Translational cancer research 11:519-529 PubMed35402183

2022

CircPTN promotes angiogenesis via the MiR-595/LYRM5 signaling pathway in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shengcong Guo,Zejun Mao,Guodong Zhang,Botao Xu,Xiangfeng He
View all publications
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