Anti-Lysophospholipase 1/LPL-I antibody [EPR3667]

• WB

Lab

Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (AB91606)

Lanes 1- 2 : Merged signal (red and green). Green - ab91606 observed at 25 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab91606 was shown to react with Lysophospholipase 1/LPL-I in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266195 (knockout cell lysate ab257509) was used. Wild-type HEK-293T and LYPLA1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab91606 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606) at 1/5000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

LYPLA1 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human LYPLA1 (Lysophospholipase 1/LPL-I) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-lypla1-lysophospholipase-1-lpl-i-knockout-hek-293t-cell-line-ab266195'>ab266195</a>)

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

• WB

Lab

Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (AB91606)

Lane 1 : Wild-type HAP1 whole cell lysate (40 μg)
Lane 2 : LYPLA1 knockout HAP1 whole cell lysate (40 μg)
Lanes 1 - 2 : Merged signal (red and green). Green - ab91606 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab91606 was shown to specifically react with LYPLA1 in wild-type HAP1 cells. No band was obsereved when LYPLA1 knockout samples were examined. Wild-type and LYPLA1 knockout samples were subjected to SDS-PAGE. ab91606 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606)

Predicted band size: 25 kDa

false

• WB

Unknown

Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (AB91606)

All lanes:

Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606) at 1/5000 dilution

Lane 1:

Fetal liver tissue lysate at 10 µg

Lane 2:

Fetal heart tissue lysate at 10 µg

Lane 3:

Human spleen tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/1000 dilution

Predicted band size: 25 kDa

false

• WB

Lab

Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (AB91606)

All lanes:

Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606) at 1/5000 dilution

Lane 1:

Human liver tissue lysates at 20 µg

Lane 2:

Human fetal heart tissue lysates at 20 µg

Lane 3:

Mouse liver tissue lysates at 20 µg

Lane 4:

Rat liver tissue lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

• WB

Unknown

Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (AB91606)

All lanes:

Western blot - Anti-Lysophospholipase 1/LPL-I antibody [EPR3667] (ab91606) at 1/5000 dilution

Lane 1:

Mouse heart lysate at 10 µg

Lane 2:

Mouse kidney lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Lane 4:

Rat kidney lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/1000 dilution

Predicted band size: 25 kDa

false