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Mouse Monoclonal Lysosomal acid lipase/LAL antibody. Suitable for IP, WB, IHC-P and reacts with Transfected cell lysate, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LIPA.

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Images

Western blot - Anti-Lysosomal acid lipase/LAL antibody [1F9] (AB219113), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysosomal acid lipase/LAL antibody [1F9] (AB219113), expandable thumbnail
  • Immunoprecipitation - Anti-Lysosomal acid lipase/LAL antibody [1F9] (AB219113), expandable thumbnail
  • Western blot - Anti-Lysosomal acid lipase/LAL antibody [1F9] (AB219113), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human LIPA. Database link P38571

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Expected
Tested
Tested
Cynomolgus monkey
Predicted
Predicted
Predicted
Transfected cell lysate
Tested
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cynomolgus monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Cynomolgus monkey
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
10 µg/mL
Notes

-

Predicted
Predicted

Species
Cynomolgus monkey
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Catalyzes the deacylation of cholesteryl ester core lipids of endocytosed low density lipoproteins to generate free fatty acids and cholesterol (PubMed:15269241, PubMed:1718995, PubMed:7204383, PubMed:8112342, PubMed:9633819). Hydrolyzes triglycerides (1,2,3-triacylglycerol) and diglycerides (such as 1,2-diacylglycerol and 1,3-diacylglycerol) with preference for the acyl moieties at the sn-1 or sn-3 positions (PubMed:7204383, PubMed:8112342).

Alternative names

Lysosomal acid lipase/cholesteryl ester hydrolase, Acid cholesteryl ester hydrolase, LAL, Cholesteryl esterase, Diacylglycerol lipase, Lipase A, Sterol esterase, Triacylglycerol ester hydrolase, Triacylglycerol lipase, LIPA

Recommended products

Mouse Monoclonal Lysosomal acid lipase/LAL antibody. Suitable for IP, WB, IHC-P and reacts with Transfected cell lysate, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LIPA.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human LIPA. Database link P38571
Clone number
1F9
Purification technique
Affinity purification Protein A
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Lysosomal acid lipase (LAL) also called lipase A is an essential enzyme with a mass of approximately 43 kDa. It is expressed within the lysosomes of many cell types and catalyzes the hydrolysis of cholesteryl esters and triglycerides into free cholesterol and free fatty acids. This process is important for the cellular lipid metabolism which provides essential signaling molecules and membrane components. The enzyme's activity varies across different tissue types with high expression levels observed in organs like the liver and the adrenal glands.

Biological function summary

Lysosomal acid lipase contributes to the breakdown and regulation of lipid storage within the cell. It is not known to be part of a larger protein complex but its function is critical for maintaining lipid homeostasis in the cell. Through its actions LAL supplies cholesterol and fatty acids essential for various cellular processes including membrane formation and energy production. Additionally it assists in cellular storage management by preventing the excessive accumulation of lipids.

Pathways

The enzymatic actions of lysosomal acid lipase are central to the cholesterol ester and triglyceride metabolic pathways. It plays an important role in the lysosome as part of the lipid degradation process impacting cholesterol transport pathways and maintaining lipid equilibrium. Its activities are closely related to those of other enzymes like hormone-sensitive lipase which also participate in lipid metabolism though within distinct compartments and contexts.

Associated diseases and disorders

Lysosomal acid lipase is linked to lipid storage disorders such as Wolman disease and Cholesteryl Ester Storage Disease (CESD). These conditions result from LAL deficiency leading to harmful accumulation of cholesteryl esters and triglycerides in tissues. Through disease pathways LAL becomes interconnected with proteins affected by lipid dysregulation such as low-density lipoprotein receptors which play a part in maintaining lipid balance and can contribute to more severe cardiovascular conditions when impaired by LAL deficiency.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Lysosomal acid lipase/LAL antibody [1F9] (ab219113), expandable thumbnail

    Western blot - Anti-Lysosomal acid lipase/LAL antibody [1F9] (ab219113)

    All lanes: Western blot - Anti-Lysosomal acid lipase/LAL antibody [1F9] (ab219113)

    Lane 1: Lysosomal acid lipase/LAL transfected 293T lysate

    Lane 2: Non-transfected 293T lysate

    Predicted band size: 45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysosomal acid lipase/LAL antibody [1F9] (ab219113), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysosomal acid lipase/LAL antibody [1F9] (ab219113)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labeling Lysosomal acid lipase/LAL with ab219113 at 10μg/ml.

  • Immunoprecipitation - Anti-Lysosomal acid lipase/LAL antibody [1F9] (ab219113), expandable thumbnail

    Immunoprecipitation - Anti-Lysosomal acid lipase/LAL antibody [1F9] (ab219113)

    Immunoprecipitation analysis of Lysosomal acid lipase/LAL transfected lysate using ab219113 and Protein A Magnetic Bead, and immunoblotted with Lysosomal acid lipase/LAL rabbit polyclonal antibody

    All lanes: Immunoprecipitation - Anti-Lysosomal acid lipase/LAL antibody [1F9] (ab219113)

    Predicted band size: 45 kDa, 57 kDa

    Observed band size: 35 kDa, 57 kDa

  • Western blot - Anti-Lysosomal acid lipase/LAL antibody [1F9] (ab219113), expandable thumbnail

    Western blot - Anti-Lysosomal acid lipase/LAL antibody [1F9] (ab219113)

    Western blot: Anti-LIPA antibody [1F9] (ab219113) staining at 1/1000 dilution, shown in green; Rabbit anti-ACTN4 [EPR2533(2)] (Anti-alpha Actinin 4 antibody [EPR2533(2)] ab108198) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab219113 was shown to bind specifically to LIPA. A band was observed at 54 kDa in wild-type A549 cell lysates with no signal observed at this size in LIPA knockout cell line. To generate this image, wild-type and LIPA knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-Lysosomal acid lipase/LAL antibody [1F9] (ab219113) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: LIPA knockout A549 cell lysate at 20 µg

    Secondary

    Lanes 1 - 2: Goat anti-Mouse IgG H&L 800CW at 1/20000 dilution

    Lanes 1 - 2: Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 54 kDa

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Product protocols

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