AB219113

Anti-Lysosomal acid lipase/LAL antibody [1F9]

Name: Anti-Lysosomal acid lipase/LAL antibody [1F9] (ab219113) | Abcam
Brand: Abcam Limited
SKU: AB219113
Price: 735 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal Lysosomal acid lipase/LAL antibody. Suitable for IP, WB, IHC-P and reacts with Transfected cell lysate, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LIPA.

View Alternative Names

Lysosomal acid lipase/cholesteryl ester hydrolase, Acid cholesteryl ester hydrolase, LAL, Cholesteryl esterase, Diacylglycerol lipase, Lipase A, Sterol esterase, Triacylglycerol ester hydrolase, Triacylglycerol lipase, LIPA

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysosomal acid lipase/LAL antibody [1F9] (AB219113)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysosomal acid lipase/LAL antibody [1F9] (AB219113)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labeling Lysosomal acid lipase/LAL with ab219113 at 10μg/ml.

Lab

Western blot - Anti-Lysosomal acid lipase/LAL antibody [1F9] (AB219113)

Western blot : Anti-LIPA antibody [1F9] (ab219113) staining at 1/1000 dilution, shown in green; Rabbit anti-ACTN4 [EPR2533(2)] (ab108198) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab219113 was shown to bind specifically to LIPA. A band was observed at 54 kDa in wild-type A549 cell lysates with no signal observed at this size in LIPA knockout cell line (ab326020). To generate this image, wild-type and LIPA knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Lysosomal acid lipase/LAL antibody [1F9] (ab219113) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

Western blot - Human LIPA knockout A549 cell line (<a href='/en-us/products/cell-lines/human-lipa-knockout-a549-cell-line-ab326020'>ab326020</a>) at 20 µg

Secondary

Lanes 1 - 2:

Goat anti-Mouse IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 2:

Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

Observed band size: 54 kDa

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Supplier Data

Immunoprecipitation - Anti-Lysosomal acid lipase/LAL antibody [1F9] (AB219113)

Immunoprecipitation analysis of Lysosomal acid lipase/LAL transfected lysate using ab219113 and Protein A Magnetic Bead, and immunoblotted with Lysosomal acid lipase/LAL rabbit polyclonal antibody

All lanes:

Immunoprecipitation - Anti-Lysosomal acid lipase/LAL antibody [1F9] (ab219113)

Predicted band size: 45 kDa,57 kDa

Observed band size: 35 kDa,57 kDa

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Supplier Data

Western blot - Anti-Lysosomal acid lipase/LAL antibody [1F9] (AB219113)

All lanes:

Western blot - Anti-Lysosomal acid lipase/LAL antibody [1F9] (ab219113)

Lane 1:

Lysosomal acid lipase/LAL transfected 293T lysate

Lane 2:

Non-transfected 293T lysate

Predicted band size: 45 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1F9

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P, IP, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human LIPA.

P38571

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lysosomal acid lipase (LAL) also called lipase A is an essential enzyme with a mass of approximately 43 kDa. It is expressed within the lysosomes of many cell types and catalyzes the hydrolysis of cholesteryl esters and triglycerides into free cholesterol and free fatty acids. This process is important for the cellular lipid metabolism which provides essential signaling molecules and membrane components. The enzyme's activity varies across different tissue types with high expression levels observed in organs like the liver and the adrenal glands.

Biological function summary

Lysosomal acid lipase contributes to the breakdown and regulation of lipid storage within the cell. It is not known to be part of a larger protein complex but its function is critical for maintaining lipid homeostasis in the cell. Through its actions LAL supplies cholesterol and fatty acids essential for various cellular processes including membrane formation and energy production. Additionally it assists in cellular storage management by preventing the excessive accumulation of lipids.

Pathways

The enzymatic actions of lysosomal acid lipase are central to the cholesterol ester and triglyceride metabolic pathways. It plays an important role in the lysosome as part of the lipid degradation process impacting cholesterol transport pathways and maintaining lipid equilibrium. Its activities are closely related to those of other enzymes like hormone-sensitive lipase which also participate in lipid metabolism though within distinct compartments and contexts.

Lysosomal acid lipase is linked to lipid storage disorders such as Wolman disease and Cholesteryl Ester Storage Disease (CESD). These conditions result from LAL deficiency leading to harmful accumulation of cholesteryl esters and triglycerides in tissues. Through disease pathways LAL becomes interconnected with proteins affected by lipid dysregulation such as low-density lipoprotein receptors which play a part in maintaining lipid balance and can contribute to more severe cardiovascular conditions when impaired by LAL deficiency.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Catalyzes the deacylation of cholesteryl ester core lipids of endocytosed low density lipoproteins to generate free fatty acids and cholesterol (PubMed : 15269241, PubMed : 1718995, PubMed : 7204383, PubMed : 8112342, PubMed : 9633819). Hydrolyzes triglycerides (1,2,3-triacylglycerol) and diglycerides (such as 1,2-diacylglycerol and 1,3-diacylglycerol) with preference for the acyl moieties at the sn-1 or sn-3 positions (PubMed : 7204383, PubMed : 8112342).

See full target information LIPA

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 56:697-708 PubMed31922217

2020

PAX2 promotes epithelial ovarian cancer progression involving fatty acid metabolic reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Feng,Yong Tang,Yannan Mao,Yingzhao Liu,Desheng Yao,Linkai Yang,Kenneth Garson,Barbara C Vanderhyden,Qi Wang

Molecular medicine reports 20:3406-3414 PubMed31432175

2019

Overexpression of the medium‑conductance calcium‑activated potassium channel (SK4) and the HCN2 channel to generate a biological pacemaker.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyi Zhao,Mei Yang,Fengyuan Wang,Ankang Yang,Qingyan Zhao,Xi Wang,Yanhong Tang,Teng Wang,Congxin Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Lysosomal acid lipase/LAL antibody [1F9]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysosomal acid lipase/LAL antibody [1F9] (AB219113)

Western blot - Anti-Lysosomal acid lipase/LAL antibody [1F9] (AB219113)

Immunoprecipitation - Anti-Lysosomal acid lipase/LAL antibody [1F9] (AB219113)

Western blot - Anti-Lysosomal acid lipase/LAL antibody [1F9] (AB219113)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise