AB154356

Anti-Lysosomal acid lipase/LAL antibody

Name: Anti-Lysosomal acid lipase/LAL antibody (ab154356) | Abcam
Brand: Abcam Limited
SKU: AB154356
Price: 220 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal Lysosomal acid lipase/LAL antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LIPA aa 150 to C-terminus.

View Alternative Names

Lysosomal acid lipase/cholesteryl ester hydrolase, Acid cholesteryl ester hydrolase, LAL, Cholesteryl esterase, Diacylglycerol lipase, Lipase A, Sterol esterase, Triacylglycerol ester hydrolase, Triacylglycerol lipase, LIPA

2 Images

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Western blot - Anti-Lysosomal acid lipase/LAL antibody (AB154356)

10% SDS PAGE

All lanes:

Western blot - Anti-Lysosomal acid lipase/LAL antibody (ab154356) at 1/1000 dilution

All lanes:

Mouse liver whole cell lysate at 50 µg

Predicted band size: 45 kDa

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Western blot - Anti-Lysosomal acid lipase/LAL antibody (AB154356)

10% SDS PAGE

All lanes:

Western blot - Anti-Lysosomal acid lipase/LAL antibody (ab154356) at 1/1000 dilution

Lane 1:

A549 whole cell lysate at 30 µg

Lane 2:

H1299 whole cell lysate at 30 µg

Predicted band size: 45 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human LIPA aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P38571

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lysosomal acid lipase (LAL) also called lipase A is an essential enzyme with a mass of approximately 43 kDa. It is expressed within the lysosomes of many cell types and catalyzes the hydrolysis of cholesteryl esters and triglycerides into free cholesterol and free fatty acids. This process is important for the cellular lipid metabolism which provides essential signaling molecules and membrane components. The enzyme's activity varies across different tissue types with high expression levels observed in organs like the liver and the adrenal glands.

Biological function summary

Lysosomal acid lipase contributes to the breakdown and regulation of lipid storage within the cell. It is not known to be part of a larger protein complex but its function is critical for maintaining lipid homeostasis in the cell. Through its actions LAL supplies cholesterol and fatty acids essential for various cellular processes including membrane formation and energy production. Additionally it assists in cellular storage management by preventing the excessive accumulation of lipids.

Pathways

The enzymatic actions of lysosomal acid lipase are central to the cholesterol ester and triglyceride metabolic pathways. It plays an important role in the lysosome as part of the lipid degradation process impacting cholesterol transport pathways and maintaining lipid equilibrium. Its activities are closely related to those of other enzymes like hormone-sensitive lipase which also participate in lipid metabolism though within distinct compartments and contexts.

Lysosomal acid lipase is linked to lipid storage disorders such as Wolman disease and Cholesteryl Ester Storage Disease (CESD). These conditions result from LAL deficiency leading to harmful accumulation of cholesteryl esters and triglycerides in tissues. Through disease pathways LAL becomes interconnected with proteins affected by lipid dysregulation such as low-density lipoprotein receptors which play a part in maintaining lipid balance and can contribute to more severe cardiovascular conditions when impaired by LAL deficiency.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the deacylation of cholesteryl ester core lipids of endocytosed low density lipoproteins to generate free fatty acids and cholesterol (PubMed : 15269241, PubMed : 1718995, PubMed : 7204383, PubMed : 8112342, PubMed : 9633819). Hydrolyzes triglycerides (1,2,3-triacylglycerol) and diglycerides (such as 1,2-diacylglycerol and 1,3-diacylglycerol) with preference for the acyl moieties at the sn-1 or sn-3 positions (PubMed : 7204383, PubMed : 8112342).

See full target information LIPA

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 12:1092 PubMed34795230

2021

Molecular and histological traits of reduced lysosomal acid lipase activity in the fatty liver.

Applications

Unspecified application

Species

Unspecified reactive species

Simone Carotti,Daniele Lettieri-Barbato,Fiorella Piemonte,Sergio Ruggiero,Marco Rosina,Francesca Zalfa,Maria Zingariello,Francesca Arciprete,Francesco Valentini,Maria Francesconi,Jessica D'Amico,Antonio De Vincentis,Andrea Baiocchini,Giuseppe Perrone,Raffaele Antonelli-Incalzi,Sergio Morini,Antonio Picardi,Katia Aquilano,Umberto Vespasiani-Gentilucci

eLife 9: PubMed33320092

2020

An AMPK-dependent, non-canonical p53 pathway plays a key role in adipocyte metabolic reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Wang,Xueping Wan,Paul F Pilch,Leif W Ellisen,Susan K Fried,Libin Liu

Nature metabolism 1:1127-1140 PubMed32577608

2019

Autophagic lipid metabolism sustains mTORC1 activity in TSC-deficient neural stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chenran Wang,Michael A Haas,Fuchun Yang,Syn Yeo,Takako Okamoto,Song Chen,Jian Wen,Pranjal Sarma,David R Plas,Jun-Lin Guan

PloS one 10:e0131995 PubMed26134519

2015

A Fine Balance of Dietary Lipids Improves Pathology of a Murine Model of VCP-Associated Multisystem Proteinopathy.

Applications

Species

Mouse

Katrina J Llewellyn,Naomi Walker,Christopher Nguyen,Baichang Tan,Lbachir BenMohamed,Virginia E Kimonis,Angèle Nalbandian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Lysosomal acid lipase/LAL antibody

Western blot - Anti-Lysosomal acid lipase/LAL antibody (AB154356)

Western blot - Anti-Lysosomal acid lipase/LAL antibody (AB154356)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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