Anti-Lysosomal acid lipase/LAL antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Lysosomal acid lipase/LAL antibody (AB236736)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Lysosomal acid lipase/LAL (green) using ab236736 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysosomal acid lipase/LAL antibody (AB236736)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Lysosomal acid lipase/LAL with ab236736 at 1/50 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysosomal acid lipase/LAL antibody (AB236736)

Immunohistochemical analysis of paraffin-embedded Human colorectal cancer tissue labeling Lysosomal acid lipase/LAL with ab236736 at 1/50 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody.

• WB

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Western blot - Anti-Lysosomal acid lipase/LAL antibody (AB236736)

All lanes:

Western blot - Anti-Lysosomal acid lipase/LAL antibody (ab236736) at 1/1000 dilution

Lane 1:

MCF7 whole cell lysate

Lane 2:

293T whole cell lysate

Lane 3:

PC-3 whole cell lysate

Lane 4:

U251 whole cell lysate

Lane 5:

A549 whole cell lysate

Lane 6:

HeLa whole cell lysate

Secondary

All lanes:

Western blot at 1/50000 dilution

Observed band size: 46 kDa

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