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AB89771

Anti-Lysosomal acid lipase/LAL antibody

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Mouse Polyclonal Lysosomal acid lipase/LAL antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LIPA.

View Alternative Names

Lysosomal acid lipase/cholesteryl ester hydrolase, Acid cholesteryl ester hydrolase, LAL, Cholesteryl esterase, Diacylglycerol lipase, Lipase A, Sterol esterase, Triacylglycerol ester hydrolase, Triacylglycerol lipase, LIPA

2 Images
Western blot - Anti-Lysosomal acid lipase/LAL antibody (AB89771)
  • WB

Unknown

Western blot - Anti-Lysosomal acid lipase/LAL antibody (AB89771)

All lanes:

Western blot - Anti-Lysosomal acid lipase/LAL antibody (ab89771) at 1 µg/mL

All lanes:

human pancreas tissue lysate at 50 µg

Predicted band size: 45 kDa

Observed band size: 45 kDa

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Western blot - Anti-Lysosomal acid lipase/LAL antibody (AB89771)
  • WB

Unknown

Western blot - Anti-Lysosomal acid lipase/LAL antibody (AB89771)

All lanes:

Western blot - Anti-Lysosomal acid lipase/LAL antibody (ab89771) at 1 µg/mL

Lane 1:

Lysosomal acid lipase/LAL transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected lysate at 25 µg

Predicted band size: 45 kDa

Observed band size: 45 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human LIPA.

P38571

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lysosomal acid lipase (LAL) also called lipase A is an essential enzyme with a mass of approximately 43 kDa. It is expressed within the lysosomes of many cell types and catalyzes the hydrolysis of cholesteryl esters and triglycerides into free cholesterol and free fatty acids. This process is important for the cellular lipid metabolism which provides essential signaling molecules and membrane components. The enzyme's activity varies across different tissue types with high expression levels observed in organs like the liver and the adrenal glands.
Biological function summary

Lysosomal acid lipase contributes to the breakdown and regulation of lipid storage within the cell. It is not known to be part of a larger protein complex but its function is critical for maintaining lipid homeostasis in the cell. Through its actions LAL supplies cholesterol and fatty acids essential for various cellular processes including membrane formation and energy production. Additionally it assists in cellular storage management by preventing the excessive accumulation of lipids.

Pathways

The enzymatic actions of lysosomal acid lipase are central to the cholesterol ester and triglyceride metabolic pathways. It plays an important role in the lysosome as part of the lipid degradation process impacting cholesterol transport pathways and maintaining lipid equilibrium. Its activities are closely related to those of other enzymes like hormone-sensitive lipase which also participate in lipid metabolism though within distinct compartments and contexts.

Lysosomal acid lipase is linked to lipid storage disorders such as Wolman disease and Cholesteryl Ester Storage Disease (CESD). These conditions result from LAL deficiency leading to harmful accumulation of cholesteryl esters and triglycerides in tissues. Through disease pathways LAL becomes interconnected with proteins affected by lipid dysregulation such as low-density lipoprotein receptors which play a part in maintaining lipid balance and can contribute to more severe cardiovascular conditions when impaired by LAL deficiency.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the deacylation of cholesteryl ester core lipids of endocytosed low density lipoproteins to generate free fatty acids and cholesterol (PubMed : 15269241, PubMed : 1718995, PubMed : 7204383, PubMed : 8112342, PubMed : 9633819). Hydrolyzes triglycerides (1,2,3-triacylglycerol) and diglycerides (such as 1,2-diacylglycerol and 1,3-diacylglycerol) with preference for the acyl moieties at the sn-1 or sn-3 positions (PubMed : 7204383, PubMed : 8112342).
See full target information LIPA
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