Anti-Lysosomal acid lipase/LAL antibody [EPR29160-155]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysosomal acid lipase/LAL antibody [EPR29160-155] (AB323282)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Lysosomal acid lipase/LAL with ab323282 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human hepatocellular carcinoma.
The section was incubated with ab323282 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysosomal acid lipase/LAL antibody [EPR29160-155] (AB323282)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Lysosomal acid lipase/LAL with ab323282 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human colon carcinoma.
The section was incubated with ab323282 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysosomal acid lipase/LAL antibody [EPR29160-155] (AB323282)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Lysosomal acid lipase/LAL with ab323282 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human placenta.
The section was incubated with ab323282 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysosomal acid lipase/LAL antibody [EPR29160-155] (AB323282)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Lysosomal acid lipase/LAL with ab323282 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human lung.
The section was incubated with ab323282 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Lysosomal acid lipase/LAL antibody [EPR29160-155] (AB323282)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1-4 : 26 seconds; Lane 5-6 : 5 seconds

All lanes:

Western blot - Anti-Lysosomal acid lipase/LAL antibody [EPR29160-155] (ab323282) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 30 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 30 µg

Lane 3:

Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 30 µg

Lane 4:

HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 30 µg

Lane 5:

Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 30 µg

Lane 6:

THP-1 treated with 100ng/ml PMA for 72 hours, whole cell lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 45 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-Lysosomal acid lipase/LAL antibody [EPR29160-155] (AB323282)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Human skeletal muscle, human liver (PMID : 8112342).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Lysosomal acid lipase/LAL antibody [EPR29160-155] (ab323282) at 1/1000 dilution

Lane 1:

Human spleen tissue lysate at 30 µg

Lane 2:

Human lung tissue lysate at 30 µg

Lane 3:

Human skeletal muscle tissue lysate at 30 µg

Lane 4:

Human liver tissue lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 45 kDa,36 kDa

false

Exposure time: 15s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysosomal acid lipase/LAL antibody [EPR29160-155] (AB323282)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Lysosomal acid lipase/LAL with ab323282 at 1/100 (5.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : Nearly no staining in human skeletal muscle.
The section was incubated with ab323282 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins